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Acta Physiologiae Plantarum

, 33:2437 | Cite as

Differential expression of proteins in maize roots in response to abscisic acid and drought

  • Xiuli HuEmail author
  • Minghui Lu
  • Chaohao Li
  • Tianxue Liu
  • Wei Wang
  • Jianyu Wu
  • Fuju Tai
  • Xiao Li
  • Jie Zhang
Original Paper

Abstract

Roots are highly sensitive organ in plant response to drought, which commonly inhibits root growth. However, less is known about the effect of ABA on root protein expression induced by drought. To help clarify the role of ABA in protein expression of root response to drought, root protein patterns were monitored using a proteomic approach in maize ABA-deficient mutant vp5 and its wild-type Vp5 exposed to drought. Two-dimensional electrophoresis was used to identify drought-responsive protein spots in maize roots. After coomassie brilliant blue staining, approximately 450 protein spots were reproducibly detected on each gel, wherein 22 protein spots related to ABA or drought were identified using MALDI-TOF MS. Results showed that the 22 proteins are involved in such several cellular processes as energy and metabolism, redox homeostasis and regulatory. An anionic peroxidase and two putative uncharacterized proteins were up-regulated by drought in ABA-dependent way; A glycine-rich RNA binding protein 2, pathogenesis-related protein 10, an enolase, a serine/threonine-protein kinase receptor and a cytosolic ascorbate peroxidase were up-regulated by drought in both ABA-dependent and ABA-independent way; a nuclear transport factor 2, a nucleoside diphosphate kinase, a putative uncharacterized protein and a peroxiredoxin-5 were up-regulated by drought in ABA-independent way; a superoxide dismutase 4A, a VAP27-2, a transcription factor BTF3, a glutathione S-transferase GSTF2 and a putative uncharacterized protein were up-regulated by drought in ABA-dependent way, but not exogenous ABA treatment in the absence of drought; a O-methyltransferase and a putative uncharacterized proteins were down-regulated by ABA and drought. The identification of some novel proteins in the drought response provides new insights that can lead to a better understanding of the molecular basis of root drought tolerance.

Keywords

ABA Drought stress Roots Zea mays L. Proteomics 

Abbreviations

ABA

Abscisic acid

APX

Ascorbate peroxidase

APRX

Anionic peroxidase

CBB

Coomassie brilliant blue

2-DE

Two-dimensional electrophoresis

DTT

Dithiothreitol

GRP2

Glycine-rich RNA binding protein 2

GST

Glutathione S-transferase

IEF

Isoelectric focusing

JA

Jasmonate

MALDI-TOF

Matrix-assisted laser desorption/ionization time of flight

MS

Mass spectrometry

NTF2

Nuclear transport factor 2

NDPKs

Nucleoside diphosphate kinases

PMSF

Phenylmethanesulfonyl fluoride

PVP

Polyvinylpyrrolidone

PVPP

Polyvinylpolypyrrolidone

pI

Isoelectric point

SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

TCA

Trichloroacetic acid

TFA

Trifluoroacetic

OMT

O-methyltransferase

Prxs

Peroxiredoxin

ROS

Reactive oxygen species

SA

Salicylic acid

SOD

Superoxide dismutase

ZmPR10

Maize pathogenesis-related protein 10

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 30800667 to XL Hu), the Fok Ying-Tong Education Foundation, China (Grant No. 122032), the China Postdoctoral Science Foundation (Grant no. 20080440824 and No. 200902357 to XL Hu), the Foundation for University Key Teacher by the Ministry of Education (grant No.2009GGJS-028 to XL Hu) and the Foundation of Henan Major Public Projects (Grant No.091100910100).

References

  1. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399PubMedCrossRefGoogle Scholar
  2. Buchanan CD, Lim S, Salzman RA, Kagiampakis I, Morishige DT, Weers BD, Klein RR, Pratt LH, Cordonnier-Pratt MM, Klein PE, Mullet JE (2005) Sorghum bicolor’s transcriptome response to dehydration, high salinity and ABA. Plant Mol Biol 5:699–720CrossRefGoogle Scholar
  3. Cho SM, Shin SH, Kim KS, Kim YC, Eun MY, Cho BH (2004) Enhanced expression of a gene encoding a nucleoside diphosphate kinase 1 (OsNDPK1) in rice plants upon infection with bacterial pathogens. Mol Cell 18:390–395Google Scholar
  4. Comstock JP (2002) Hydraulic and chemical signalling in the control of stomatal conductance and transpiration. J Exp Bot 53:195–200PubMedCrossRefGoogle Scholar
  5. Custers JH, Melchers LS, Tigelaar H, Bade JB, Spiegeler JJ, van Der Meijs PJ, Simons BH, Stuiver MH (2002) T-DNA tagging of a pathogen inducible promoter in Arabidopsis thaliana. Mol Plant Pathol 3:239–249PubMedCrossRefGoogle Scholar
  6. Davletova S, Rizhsky L, Liang H, Zhong S, Oliver DJ, Coutu J, Shulaev V, Schlauch K, Mittler R (2005) Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis. Plant Cell 17:268–281PubMedCrossRefGoogle Scholar
  7. Dietz KJ, Jacob S, Oelze ML, Laxa M, Tognetti V, de Miranda SMN, Baier M, Finkemeier I (2006) The function of peroxiredoxins in plant organelle redox metabolism. J Exp Bot 57:1697–1709PubMedCrossRefGoogle Scholar
  8. Dooki AD, Mayer-Posner FJ, Askari H, Zaiee A, Salekdeh GH (2006) Proteomic responses of rice young panicles to salinity. Proteomics 6:6498–6507PubMedCrossRefGoogle Scholar
  9. Dubos C, Plomion C (2001) Drought differentially affects expression of a PR-10 protein, in needles of the maritime pine (Pinus pinaster Ait.) seedlings. J Exp Bot 52:1143–1154PubMedCrossRefGoogle Scholar
  10. Forsthoefel NR, Cushman MAF, Cushman JC (1995) Posttranscriptional and posttranslational control of enolase expression in the facultative grassulacean acid metabolism plant Mesembryanthemum crystallinum L. Plant Physiol 108:1185–1195PubMedCrossRefGoogle Scholar
  11. Gorantla M, Babu PR, Lachagari VBR, Feltus FA, Paterson AH, Reddy AR (2005) Functional genomics of drought-stress response in rice: transcript mapping of annotated unigenes of an indica rice (Oryza sativa L. cv. Nagina 22). Curr Sci 289:496–514Google Scholar
  12. Guan LQ, Scandalios JG (1998) Two structurally similar maize cytosolic superoxide dismutase genes, Sod4 and Sod4A, respond differentially to abscisic acid and high osmoticum. Plant Physiol 117:217–224PubMedCrossRefGoogle Scholar
  13. Guillet-Claude C, Birolleau-Touchard C, Manicacci D, Fourmann M, Barraud S, Carret V, Martinant JP, Barrie` re Y (2004) Genetic diversity associated with variation in silage corn digestibility for three O-methyltransferase genes involved in lignin biosynthesis. Theor Appl Genet 110:126–135PubMedCrossRefGoogle Scholar
  14. Hajheidari M, Salekdeh GH, Heidari M, Abdollahian-Noghabi M, Sadeghian SY (2005) Proteome analysis of sugar beet leaves under drought stress. Proteomics 5:950–960PubMedCrossRefGoogle Scholar
  15. Halusková L, Valentovicová K, Huttová J, Mistrík I, Tamás L (2009) Effect of abiotic stresses on glutathione peroxidase and glutathione S-transferase activity in barley root tips. Plant Physiol Biochem 47:1069–1074PubMedCrossRefGoogle Scholar
  16. Hu X, Li Y, Li C, Yang H, Wang W, Lu M (2010a) Characterization of small heat shock proteins associated with maize tolerance to combined drought and heat stress. J Plant Growth Regul 29:455–464CrossRefGoogle Scholar
  17. Hu X, Liu R, Li Y, Wang W, Tai F, Xue R, Li C (2010b) Heat shock protein 70 regulates the abscisic acid-induced antioxidant response of maize to drought and heat stress combination. Plant Growth Regul 60:225–235CrossRefGoogle Scholar
  18. Jeong JS, Kim YS, Baek KH, Jung H, Ha SH, Do Choi Y, Kim M, Reuzeau C, Kim JK (2010) Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions. Plant Physiol 153:185–197PubMedCrossRefGoogle Scholar
  19. Jiang HW, Liu MJ, Chen IC, Huang CH, Chao LY, Hsieh HL (2010) A glutathione S-transferase regulated by light and hormones participates in the modulation of Arabidopsis seedling development. Plant Physiol 154:1646–1658PubMedCrossRefGoogle Scholar
  20. Kellos T, Tímár I, Szilágyi V, Szalai G, Galiba G, Kocsy G (2008) Stress hormones and abiotic stresses have different effects on antioxidants in maize lines with different sensitivity. Plant Biol (Stuttg) 10:563–572CrossRefGoogle Scholar
  21. Kim JY, Park SJ, Jang B, Jung CHH, Ahn SJ, Goh CHH, Cho K, Han O, Kang H (2007) Functional characterization of a glycine-rich RNA-binding protein2 in Arabidopsis thaliana under abiotic stress conditions. Plant J 50:439–451PubMedCrossRefGoogle Scholar
  22. Koussevitzky S, Suzuki N, Huntington S, Armijo L, Sha W, Cortes D, Shulaev V, Mittler R (2008) Ascorbate peroxidase 1 plays a key role in the response of Arabidopsis thaliana to stress combination. J Biol Chem 283:34197–34203PubMedCrossRefGoogle Scholar
  23. Kwak KJ, Kim YO, Kang H (2005) Characterization of transgenic Arabidopsis plants overexpressing GR-RBP4 under high salinity, dehydration, or cold stress. J Exp Bot 56:3007–3016PubMedCrossRefGoogle Scholar
  24. Lee J, Parthier B, LiJbler M (1996) Jasmonate signalling can be uncoupled from abscisic acid signaling in barley: identification of jasmonate-regulated transcripts which are not induced by abscisic acid. Planta 199:625–632PubMedCrossRefGoogle Scholar
  25. Lee DG, Ahsan N, Lee SH, Kang KY, Bahk JD, Lee IJ, Lee BH (2007) A proteomic approach in analyzing heat-responsive proteins in rice leaves. Proteomics 7:3369–3383PubMedCrossRefGoogle Scholar
  26. Liu X, Huang B, Lin J, Fei J, Chen Z, Pang Y, Sun XF, Tang KX (2006) A novel pathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic and antimicrobial activity is stress- and pathogen-inducible. J Plant Physiol 163:546–556PubMedCrossRefGoogle Scholar
  27. Mangeon A, Junqueira RM, Sachetto-Martins G (2010) Functional diversity of the plant glycine-rich proteins superfamily. Plant Signal Behav 5:99–104PubMedCrossRefGoogle Scholar
  28. Moons A, Prinsen E, Bauw G, Van Montagu M (1997) Antagonistic effects of abscisic acid and jasmonates on salt stress-inducible transcripts in rice roots. Plant Cell 9:2243–2259PubMedCrossRefGoogle Scholar
  29. Mowla SB, Thomson JA, Farrant JM, Mundree SG (2002) A novel stress-inducible antioxidant enzyme identified from the resurrection plant Xerophyta viscosa Baker. Planta 215:716–726PubMedCrossRefGoogle Scholar
  30. Roberts E, Kolattukudy PE (1989) Molecular cloning, nucleotide sequence, and abscisic acid induction of a suberization-associated highly anionic peroxidase. Mol Gen Genet 217(2–3):223–232. doi: 10.1007/BF02464885 PubMedCrossRefGoogle Scholar
  31. Robichaud CS, Wang J, Sussex IM (1980) Control of in vitro growth of viviparous embryo mutants of maize by abscisic acid. Dev Genet 1:325–330CrossRefGoogle Scholar
  32. Salekdeh GH, Siopongco J, Wade LJ, Ghareyazie B, Bennett J (2002) Proteomic analysis of rice leaves during drought stress and recovery. Proteomics 2:1131–1145PubMedCrossRefGoogle Scholar
  33. Shao HB, Chu LY, Jaleel CA, Zhao CX (2008) Water-deficit stressinduced anatomical changes in higher plants. Comptes Rendus Biologies 331:215–225PubMedCrossRefGoogle Scholar
  34. Sharp RE, LeNoble ME (2002) ABA, ethylene and the control of shoot and root growth under water stress. J Exp Bot 53:33–37PubMedCrossRefGoogle Scholar
  35. Sharp RE, Poroyko V, Hejlek LG, Spollen WG, Springer GK, Bohnert HJ, Nguyen HT (2004) Root growth maintenance during water deficits: physiology to functional genomics. J Exp Bot 55:2343–2351PubMedCrossRefGoogle Scholar
  36. Shinozaki K, Yamaguchi-Shinozaki K (2007) Gene networks involved in drought stress response and tolerance. J Exp Bot 58:221–227PubMedCrossRefGoogle Scholar
  37. Spollen WG, LeNoble ME, Samuels TD, Bernstein N, Sharp RE (2000) Abscisic acid accumulation maintains maize primary root elongation at low water potentials by restricting ethylene production. Plant Physiol 122:967–976PubMedCrossRefGoogle Scholar
  38. Taniguchi YY, Taniguchi M, Tsuge T, Oka A, Aoyama T (2010) Involvement of Arabidopsis thaliana phospholipase Dζ2 in root hydrotropism through the suppression of root gravitropism. Planta 231:491–497PubMedCrossRefGoogle Scholar
  39. Teichmann T, Guan CH, Kristoffersen P, Muster G, Tietz O, Palme K (1997) Cloning and biochemical characterization of an anionic peroxidase from Zea mays. Eur J Biochem 247:826–832PubMedCrossRefGoogle Scholar
  40. Torres GAM, Pflieger S, Corre-Menguy F, Mazubert C, Hartmann C, Christine Lelandais-Brière C (2006) Identification of novel drought-related mRNAs in common bean roots by differential display RT-PCR. Plant Sci 171:300–307CrossRefGoogle Scholar
  41. Vincent D, Lapierre C, Pollet B, Cornic G, Negroni L, Michel Zivy M (2005) Water deficits affect caffeate O-methyltransferase, lignification, and related enzymes in maize leaves. A proteomic investigation. Plant Physiol 137:949–960PubMedCrossRefGoogle Scholar
  42. Wang W, Vignani R, Scali M, Cresti M (2006) A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis. Electrophoresis 27:2782–2786. doi: 10.1002/elps.200500722 PubMedCrossRefGoogle Scholar
  43. Wang W, Bianchi L, Scali M, Liu LW, Bini L, Cresti M (2009) Proteomic analysis of b-1, 3-glucanase in grape berry tissues. Acta Physiol Plant 31:597–604CrossRefGoogle Scholar
  44. Xie YR, Chen ZY, Brown RL, Bhatnagar D (2010) Expression and functional characterization of two pathogenesis-related protein 10 genes from Zea mays. J Plant Physiol 167:121–130PubMedCrossRefGoogle Scholar
  45. Xiong L, Wang RG, Mao GH, Koczan JM (2006) Identification of drought tolerance determinants by genetic analysis of root response to drought stress and abscisic acid. Plant Physiol 142:1065–1074PubMedCrossRefGoogle Scholar
  46. Yang L, Zheng B, Mao C, Qi X, Liu F, Wu P (2004) Analysis of transcripts that are differentially expressed in three sectors of the rice root system under water deficit. Mol Gen Genomics 272:433–442CrossRefGoogle Scholar
  47. Yazaki J, Shimatani Z, Hashimoto A, Nagata Y, Fujii F, Kojima K, Suzuki K, Taya T, Tonouchi M, Nelson C, Nakagawa A, Otomo Y, Murakami K, Matsubara K, Kawai J, Carninci P, Hayashizaki Y, Kikuchi S (2004) Transcriptional profiling of genes responsive to abscisic acid and gibberellin in rice: phenotyping and comparative analysis between rice and Arabidopsis. Physiol Genomics 17:87–100PubMedCrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2011

Authors and Affiliations

  • Xiuli Hu
    • 1
    • 2
    Email author
  • Minghui Lu
    • 1
  • Chaohao Li
    • 3
    • 4
  • Tianxue Liu
    • 1
  • Wei Wang
    • 1
  • Jianyu Wu
    • 1
  • Fuju Tai
    • 1
  • Xiao Li
    • 1
  • Jie Zhang
    • 1
  1. 1.College of Life ScienceHenan Agricultural UniversityZhengzhouChina
  2. 2.Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan ProvinceZhengzhouChina
  3. 3.College of AgronomyHenan Agricultural UniversityZhengzhouChina
  4. 4.Huanghuaihai Regional Innovation Center for Maize Technology, Ministry of AgricultureZhengzhouChina

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