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Acta Physiologiae Plantarum

, Volume 33, Issue 5, pp 2051–2056 | Cite as

Bridelia stipularis: a new source for anthocyanin production in vitro

  • V. K. Sreenivas
  • V. N. Jisha
  • Kottackal Poulose Martin
  • P. V. Madhusoodanan
Short Communication

Abstract

The present study prospects Bridelia stipularis (L.) Blume as a new source of anthocyanins through leaf and internode explants-derived callus cultures. Murashige and Skoog (MS) medium fortified with 21.48 μM α-naphthaleneacetic acid was superior for callus growth. Of the different regimes, the anthocyanin production relied on synergic effects of plant growth regulators, pH, light, and carbon source. The calluses incubated in light on MS medium with 4% glucose containing 2.22 μM N6-benzyladenine (BA) and 2.26 μM 2,4-dichlorophenoxyacetic acid (2,4-D) at pH 3.5 yielded the highest amount (a mean of 0.42 mg g−1 callus) of anthocyanins. Subsequent cultures of the calluses on the above medium yielded a stable production of anthocyanins. Medium containing glucose was superior to that with sucrose for anthocyanin formation. Kinetin was inhibitory to anthocyanin accumulation. Suspension cultures of MS medium containing 2.26 μM 2,4-D and 2.22 μM BA at pH 5.0 started excretion of anthocyanins into the medium on reaching to pH 4.4–4.6.

Keywords

Anthocyanins Callus culture Carbon source Plant growth regulators 

Abbreviations

2,4-D

2,4-Dichlorophenoxyacetic acid

BA

N6-benzyladenine

NAA

α-Naphthaleneacetic acid

Kn

Kinetin

MS

Murashige and Skoog

PGRs

Plant growth regulators

References

  1. Blando F, Scardino AP, De Bellis L, Nicoletti I, Giovinazzo G (2005) Characterization of in vitro anthocyanin-producing sour cherry (Prunus cerasus L.) callus cultures. Food Res Intl 38:937–942CrossRefGoogle Scholar
  2. Cormier F, Crevier H, Do C (1990) Effects of sucrose concentration on the accumulation of anthocyanins in grape (Vitis vinifera L.) cell suspension. Can J Bot 68:1822–1825CrossRefGoogle Scholar
  3. Eisai PT (1995) Medicinal herb index in Indonesia. PT Eisai Indonesia, Jakarta, p 91Google Scholar
  4. George EF (1993) Plant propagation by tissue culture. Part-1, Exegetics Ltd. EnglandGoogle Scholar
  5. Igarashi K, Inagaki K (1991) Effects of the major anthocyanin of wild grape (Vitis coignetiae) on serum lipid levels in rats. Agric Biol Chem 55:285–287CrossRefGoogle Scholar
  6. Igarashi K, Abe S, Sato J (1990) Effects of Atsumi-kabu (red turnip. Brassica campestris L.) anthocyanin on serum cholesterol levels in cholesterol-fed rats. Agric Biol Chem 54:171–175CrossRefGoogle Scholar
  7. Kamei H, Kojima T, Makato H, Koide T, Umeda T, Yukawa T, Terabe K (1995) Suppression of tumor cell growth by anthocyanins in vitro. Cancer Invest 13:590–594PubMedCrossRefGoogle Scholar
  8. Laleh GH, Frydoonfar H, Heidary R, Jameei R, Zare S (2006) The effect of light, temperature, pH and species on stability of anthocyanin pigments in four Berberis species. Pak J Nutri 5:90–92CrossRefGoogle Scholar
  9. Lila MA (2004) Anthocyanins and human health: an in vitro investigative approach. J Biomed Biotechnol 5:306–313CrossRefGoogle Scholar
  10. Maharik N, Elgengaihib S, Taha H (2009) Anthocyanin production in callus cultures of Crataegus sinaica Boiss. Int J Acad Res 1:30–34Google Scholar
  11. Makunga NP, van Steden J, Cress WA (1997) The effect of light and 2,4-D on the anthocyanin production in Oxalis reclinata callus. Plant Growth Regul 23:153–158CrossRefGoogle Scholar
  12. Mathur A, Mathur AK, Gangwar A, Yadav S, Verm P, Sangwan RS (2010) Anthocyanin production in a callus line of Panax sikkimensis Ban. In vitro Cell Dev Biol Plant 46:13–21CrossRefGoogle Scholar
  13. Meyer HJ, van Staden J (1995) The in vitro production of an anthocyanin from callus cultures of Oxalis linearis. Plant Cell Tiss Org Cult 40:55–58CrossRefGoogle Scholar
  14. Miura H, Kitamura Y, Ikenaga T, Mizobe K, Shimizu T, Nakamura M, Kato Y, Yamada T, Mitani T, Goda Y (1998) Anthocyanin production of Glehnia littoralis callus cultures. Phytochemistry 48:279–283PubMedCrossRefGoogle Scholar
  15. Mori T, Sakurai M, Seki M, Furusaki S (1994) Use of auxin and cytokinins to regulate anthocyanin production and composition in suspension cultures of strawberry cell. J Sci Food Agri 65:271–276CrossRefGoogle Scholar
  16. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  17. Nakamura M, Takeuchi Y, Miyanaga K, Seki M, Furusaki S (1999) High anthocyanin accumulation in the dark by strawberry (Fragaria ananassa) callus. Biotechnol Lett 21:695–699CrossRefGoogle Scholar
  18. Narayan MS, Thimmaraju R, Bhagyalakshmi N (2005) Interplay of growth regulators during solid state and liquid state batch cultivation of anthocyanin producing cell line of Daucus carota L. Process Chem 40:351–358Google Scholar
  19. Ngueyema TA, Brusotti G, Caccialanzaa G, Vita Finzi P (2009) The genus Bridelia: a phytochemical and ethnopharmacological review. J Ethnopharmacol 124:339–349CrossRefGoogle Scholar
  20. Ozeki Y, Komamine A (1986) Effect of growth regulators on the induction of anthocyanin synthesis in carrot suspension cultures. Plant Cell Physiol 27:1361–1368Google Scholar
  21. Pacheco-Palencia LA, Mertens-Talcott SU, Talcott ST (2010) In vitro absorption and antiproliferative activities of monomeric and polymeric anthocyanin fractions from açai fruit (Euterpe oleracea Mart.). Food Chem 11:1071–1078CrossRefGoogle Scholar
  22. Pool-Zobel BL, Bub A, Schroder N, Rechkemmer G (1999) Anthocyanins are potent antioxidants in model systems but do not reduce endogenous oxidative DNA damage in human colon cells. Eur J Nutr 38:227–234PubMedCrossRefGoogle Scholar
  23. Rahman MM, Ichiyanagi T, Komiyama T, Hatano Y, Konishi T (2006) Superoxide radical- and peroxynitrate-scavenging activity of anthocyanins; structure-sactivity relationship and their synergism. Free Radical Res 40:993–1002CrossRefGoogle Scholar
  24. Ram M, Prasad KV, Kaur C, Singh SK, Arora A, Kumar S (2011) Induction of anthocyanin pigments in callus cultures of Rosa hybrida L. in response to sucrose and ammonical nitrogen levels. Plant Cell Tissue Organ Cult 104:171–179CrossRefGoogle Scholar
  25. Zhang W, Furusaki S (1997) Regulation of anthocyanin synthesis in suspension cultures of strawberry cell by pH. Biotechnol Lett 19:1057–1061CrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2011

Authors and Affiliations

  1. 1.Department of BotanyUniversity of CalicutCalicutIndia
  2. 2.Department of BiotechnologyUniversity of CalicutCalicutIndia

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