Acta Physiologiae Plantarum

, Volume 33, Issue 3, pp 935–942 | Cite as

Effect of salinity on germination, phytase activity and phytate content in lettuce seedling

  • Nawel Nasri
  • Rym Kaddour
  • Mokded Rabhi
  • Claude Plassard
  • Mokhtar Lachaal
Original Paper

Abstract

Seeds of four lettuce (Lactuca sativa L.) varieties (Romaine, Augusta, Vista and Verte) differing in their salt sensitivity were sown at 0 (Control), 50, 100 and 150 mM NaCl. The final germination percentage decreased with the increasing salinity and was annulated at the highest salt concentration in Vista and Verte, the most sensitive varieties. However, in the less sensitive ones, Romaine and Augusta, it was slightly modified at 50 and 100 mM NaCl and then decreased by 50% compared with the control, at 150 mM. The effects of NaCl 100 mM on seedling growth, phytase activities, phytate and inorganic phosphorus contents were studied in Romaine and Vista showing different behaviours towards salinity. Radicle and hypocotyl length and fresh and dry weights were reduced by salt treatment in both varieties. In addition, radicle phytase activity exhibited an increase in Romaine (less sensitive) and a decrease in Vista (more sensitive). In hypocotyl, this activity showed no difference with the control in the two varieties. However, in cotyledons, and during early hours after germination, salinity decreased phytase activity in both varieties whereas in the later hours (72–96 h) this activity reached the value of the control in Romaine. The enhancement of phytase activity was concomitant with an increase in orthophosphate content and a decrease in phytate reserve. These results suggest that salt presence in the medium delays Pi remobilization from phytate stock, but stimulates assimilation of phosphorus more than its accumulation in the organs of the two lettuce varieties.

Keywords

Germination Lettuce Phytase Phytate Salinity 

References

  1. Al-Karaki GN (2001) Germination, sodium and potassium concentrations of barley seeds as influenced by salinity. J Plant Nutr 24:511–522CrossRefGoogle Scholar
  2. Almansouri M, Kinet JM, Lutts S (2001) Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.). Plant Soil 231:243–254CrossRefGoogle Scholar
  3. Beweley JD (1997) Seed germination and dormancy. Plant Cell 9:1055–1066CrossRefGoogle Scholar
  4. Bradford M (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Bioch 72:248–254CrossRefGoogle Scholar
  5. Centeno C, Viveros A, Brenes A, Canales R, Lozano A, Cuadra C (2001) Effect of several germination conditions on total P, phytate P, phytase, and acid phosphatase activities and inositol phosphate esters in rye and barley. J Agric Food Chem 49:3208–3215PubMedCrossRefGoogle Scholar
  6. Chartzoulakis K, Klapaki G (2000) Response of two greenhouse pepper hybrids to NaCl salinity during different growth stages. Sci Hortic 86:247–260CrossRefGoogle Scholar
  7. Dubey RS, Sharma KN (1990) Behaviour of phosphatases in germinating rice in relation to salt tolerance. Plant Physiol Biochem 28:17–26Google Scholar
  8. Foolad MR, Lin GY (1997) Genetic potential for salt tolerance during germination in Lycopersicon species. Hortic Sci 32:296–300Google Scholar
  9. Gopal GR, Ramaiah JK, Rao GR (1983) Influence of salinity on phytate breakdown and phytase activity in groundnut (Arachis hypogaea L.) cotyledons during germination. Natl Acad Sci Lett 6:85–87Google Scholar
  10. Greiner R, Koneitzny U, Jany K (1998) Purification and properties of a phytase from rye. J Food Biochem 22:143–161CrossRefGoogle Scholar
  11. Greiner R, Jany KD, Larsson AM (2000) Identification and purification of myo-inositol hexakisphosphate phosphohydrolases (phytases) from barley (Hordeum vulgare). J Cereal Sci 31:127–139CrossRefGoogle Scholar
  12. Hegeman CE, Grabau E (2001) A novel phytase with sequence similarity to purple acid phosphatases is expressed in cotyledons of germinating soybean seedlings. Plant Physiol 126:1598–1608PubMedCrossRefGoogle Scholar
  13. Houde RL, Alli I, Kermasha S (1990) Purification and characterization of canola seed (Brassica sp.) phytase. J Food Biochem 14:331–351CrossRefGoogle Scholar
  14. Huang J, Redman RE (1995) Salt tolerance of Hordeum and Brassica species during germination and early seedling growth. Can J Plant Sci 75:815–819Google Scholar
  15. Jamil M, Rha ES (2004) The effect of salinity (NaCl) on the germination and seedling of sugar beet (Beta vulgaris L.) and cabbage (Brassica oleracea L.). Korean J Plant Res 7:226–232Google Scholar
  16. Jamil M, Deog Bae L, Kwang Yong J, Ashraf M, Sheong Chun L, Eui Shik R (2006) Effect of salt (NaCl) stress on germination and early seedling growth of four vegetables species. J Cent Eur Agric 7:273–282Google Scholar
  17. Kashem M, Sultana N, Ikeda T, Hori H, Loboda T, Mitsui T (2000) Alteration of starch–sucrose transition in germinating wheat seed under sodium chloride salinity. J Plant Biol 43:121–127CrossRefGoogle Scholar
  18. Kebreab E, Murdoch AJ (1999) Modeling the effects of water stress and temperature on germination rate of Orobanche aegyptiaca seeds. J Exp Bot 50(335):655–664CrossRefGoogle Scholar
  19. Khan MSA, Hamid A, Karim M (1997) Effects of sodium chloride on germination and seedling characters of different types of rice (Oryza sativa L.). J Agro Crop Sci 179:163–169CrossRefGoogle Scholar
  20. Kikunaga S, Katoh Y, Takahashi M (1991) Biochemical changes in phosphorus compounds and in the activity of phytase and α-amylase in the rice grain during germination. J Sci Food Agric 56:335–343CrossRefGoogle Scholar
  21. Kreps JA, Wu Y, Chang HS, Zhu T, Wang X, Harper JF (2002) Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol 130:2129–2141PubMedCrossRefGoogle Scholar
  22. Laboure AM, Gagnon J, Lescure AM (1993) Purification and characterization of a phytases (myo-inositol hexakisphosphate phosphohydrolases) accumulated in maize (Zea mays) seedlings during germination. Biochem J 295:413–419PubMedGoogle Scholar
  23. Liao H, Wonga FL, Phanga TH, Cheunga MY, Lia WYF, Shaoa G, Yanb X, Lama HM (2003) GmPAP3, a novel purple acid phosphatase-like gene in soybean induced by NaCl stress but not phosphorus deficiency. Gene 318:103–111PubMedCrossRefGoogle Scholar
  24. Lung SC, Leung A, Kuang R, Wang Y, Leung P, Lim BL (2008) Phytase activity in tobacco (Nicotiana tabacum) root exudates is exhibited by a purple acid phosphatase. Phytochemistry 69:365–373PubMedCrossRefGoogle Scholar
  25. March JG, Villacampa AI, Grases F (1995) Enzymatic-spectrophotometric determination of phytic acid with phytase from Aspergillus ficuum. Analy Chim Acta 300:269–272CrossRefGoogle Scholar
  26. Matsui A, Ishida J, Morosawa T, Mochizuki Y, Kaminuma E, Endo TA et al (2008) Arabidopsis transcriptome analysis under drought, cold, high-salinity and ABA treatment conditions using a tiling array. Plant Cell Physiol 49:1135–1149PubMedCrossRefGoogle Scholar
  27. Maugenest S, Martinez I, Lescure AM (1997) Cloning and characterization of a cDNA encoding a maize seedlings phytase. Biochem J 322:151–157Google Scholar
  28. Mitsuhashi N, Ohnishi M, Sekiguchi Y, Kwon Y, Chang Y, Chung S, Inoue Y, Reid RJ, Yagisawa H, Mimura T (2005) Phytic acid synthesis and vacuolar accumulation in suspension-cultured cells of Catharanthus roseus induced by high concentration of inorganic phosphate and cations. Plant Physiol 138:1607–1614PubMedCrossRefGoogle Scholar
  29. Ohno T, Zibilske ML (1991) Determination of low concentrations of phosphorus in soil extracts using Malachite green. Soil Sci Soc Am J 55:892–895CrossRefGoogle Scholar
  30. Ravindram V, Ravindram G, Sivalogan S (1994) Total and phytate phosphorus contents of various foods and feed-stuffs of plant origin. Food Chem 50:133–136CrossRefGoogle Scholar
  31. Stavir K, Gupta KA, Narinder K (1998) Gibberellin A3 reverses the effect of salt stress in chickpea (Cicer arietinum L.) seedlings by enhancing amylase activity and mobilization of starch in cotyledons. Plant Growth Regul 26:85–90CrossRefGoogle Scholar
  32. Suleiman MA, ElTyeb MM, Abbass MA, Ibrahim EEA, Babiker EE, Eltinay AH (2007) Changes in chemical composition, phytate, phytase activity and minerals extractability of sprouted lentil cultivars. J Biol Sci 7:776–780CrossRefGoogle Scholar
  33. Sung HG, Shin HT, Ha JK, Lai HL, Cheng KJ, Lee JH (2005) Effect of germination temperature on characteristics of phytase production from barley. Bioresour Technol 96:1297–1303PubMedCrossRefGoogle Scholar
  34. Welbaum GE, Bradford KJ, Kyu-ock Y, Oluoch MO (1998) Biophysical, physiological and biochemical processes regulating seed germination. Seed Sci Res 8:161–172CrossRefGoogle Scholar
  35. Werner JE, Finkelstein RR (1995) Arabidopsis mutants with reduced response to NaCl and osmotic stress. Physiol Plant 93:659–666CrossRefGoogle Scholar
  36. Xiao K, Harrison MJ, Wang ZY (2005) Transgenic expression of a novel M. truncatula phytase gene results in improved acquisition of organic phosphorus by Arabidopsis. Planta 222:27–36PubMedCrossRefGoogle Scholar
  37. Zapata JP, Serrano M, Pretel MT, Amoros AM (2003) Changes in ethylene evolution and polyamines profiles of seedlings of nine cultivars of lettuce in response to salt stress during germination. Plant Sci 164:557–563CrossRefGoogle Scholar
  38. Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273PubMedCrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010

Authors and Affiliations

  • Nawel Nasri
    • 1
  • Rym Kaddour
    • 1
  • Mokded Rabhi
    • 2
  • Claude Plassard
    • 3
  • Mokhtar Lachaal
    • 1
  1. 1.Physiologie et Biochimie de la Tolérance au Sel des Plantes, Faculté des Sciences de TunisCampus UniversitaireTunisTunisia
  2. 2.Laboratoire des Plantes Extrêmophiles (LPE)Centre de Biotechnologie de Borj-CédriaHammam-LifTunisia
  3. 3.Ecologie Fonctionnelle and Biogéochimie du SolUMR 1222, INRA/IRD/SupAgro MontpellierMontpellier Cedex 1France

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