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Acta Physiologiae Plantarum

, Volume 33, Issue 3, pp 835–842 | Cite as

Exogenous hydrogen peroxide can enhance tolerance of wheat seedlings to salt stress

  • Jin-Ting Li
  • Zong-Bo Qiu
  • Xiao-Wei Zhang
  • Lin-Song Wang
Original Paper

Abstract

Hydrogen peroxide (H2O2), an active oxygen species, is widely generated in many biological systems and mediates various physiological and biochemical processes in plants. In this study, we demonstrated that exogenous H2O2 was able to improve the tolerance of wheat seedlings to salt stress. Treatments with exogenous H2O2 for 2 days significantly enhanced salt stress tolerance in wheat seedlings by decreasing the concentration of malondialdehyde (MDA), the production rate of superoxide radical (O2 ), and increasing the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), and the concentration of glutathione (GSH) and carotenoids (CAR). To further clarify the role of H2O2 in preventing salt stress damage, CAT and ascorbate (AsA), the specific H2O2 scavengers, were used. The promoting effect of exogenous H2O2 on salt stress could be reversed by the addition of CAT and AsA. It was suggested that exogenous H2O2 induced changes in MDA, O2 , antioxidant enzymes and antioxidant compounds were responsible for the increase in salt stress tolerance observed in the experiments. Therefore, H2O2 may participate in antioxidant enzymes and antioxidant compounds induced tolerance of wheat seedlings to salt stress. The results also showed that exogenous H2O2 had a positive physiological effect on the growth and development of salt-stressed seedlings.

Keywords

Hydrogen peroxide Wheat (Triticum aestivum L.Salt stress 

Notes

Acknowledgments

This work was supported by the Key Subject of Biochemistry and Molecular Biology of Henan Province and Opening Foundation of Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education.

References

  1. Abdul W, Mubaraka P, Sadia G, Shahzad MA (2007) Pretreatment of seed with H2O2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. J Plant Physiol 164:283–294CrossRefGoogle Scholar
  2. Amjad H, Salman AM, Nayyer I, Rubina A, Shafqat F (2003) Influence of hydrogen peroxide on initial leaf and coleoptile growth in etiolated wheat seedlings (Triticum aestivum L.). Asian J Plant Sci 2(15–16):1121–1125Google Scholar
  3. Amjad H, Shafqat F, Nayyer I, Rubina A (2004) Influence of exogenous application of hydrogen peroxide on root and seedling growth on wheat (Triticum aestivum L.). Int J Agric Biol 6(2):366–369Google Scholar
  4. Azevedo Neto AD, Prisco JT, Eneas-Filho J (2005) Hydrogen peroxide pre-treatment induces stress acclimation in maize plants. J Plant Physiol 162:1114–1122PubMedCrossRefGoogle Scholar
  5. Badawi GH, Yamauchi Y, Shimada E, Sasaki R, Naoyoshi K, Tanaka K (2004) Enhanced tolerance to salt stress and water deficit by overexpressing superoxide dismutase in tobacco (Nicotiana tabacum) chloroplasts. Plant Sci 166:919–928CrossRefGoogle Scholar
  6. Bowler C, Van Montagu M, Inze D (1992) Superoxide dismutase and stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 43:83–116CrossRefGoogle Scholar
  7. Cakmak I, Marschner H (1992) Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiol 98:1222–1227PubMedCrossRefGoogle Scholar
  8. Chen XY, Ding X, Xu S, Wang R, Shen WB (2009) Endogenous hydrogen peroxide plays a positive role in the upregulation of heme oxygenase and acclimation to oxidative stress in wheat seedling leaves. J Integr Plant Biol 51(10):951–960PubMedCrossRefGoogle Scholar
  9. Dat JF, Vandendede F, Vranova E, Montagu MV, Inze D, Breusegem FV (2000) Dual action of the active oxygen species during plant stress response. Cells Mol Life 57:779–795CrossRefGoogle Scholar
  10. Dionisio-Sese ML, Tobita S (1998) Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135:1–9CrossRefGoogle Scholar
  11. Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77PubMedCrossRefGoogle Scholar
  12. Elstner EF, Heupel A (1976) Inhibition of nitrite formation from hydroxylammonium-chloride: a simple assay for superoxide dismutase. Anal Biochem 70:616–620PubMedCrossRefGoogle Scholar
  13. Fedina S, Nedeva D, Çiçek N (2009) Pre-treatment with H2O2 induces salt tolerance in barley seedling. Biol Plant 53(2):321–324CrossRefGoogle Scholar
  14. Flowers TJ (1999) Salinisation and horticultural production. Sci Hortic A 78:1–4Google Scholar
  15. Giannopolitis CN, Ries SK (1977) Superoxide dismutase. 1. Occurrence in higher plants. Plant Physiol 59:309–314PubMedCrossRefGoogle Scholar
  16. Henry JF (2008) Use and abuse of exogenous H2O2 in studies of signal transduction. Free Rad Biol Med 42(7):926–932Google Scholar
  17. Hung SH, Yu CW, Lin CH (2005) Hydrogen peroxide functions as a stress signal in plants. Bot Bull Acad Sin 46:1–10Google Scholar
  18. Kovtun Y, Chiu WL, Tena G, Sheen J (2000) Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proc Natl Acad Sci USA 97:2940–2945PubMedCrossRefGoogle Scholar
  19. Li SW, Xue LG, Xu SJ, An LZ (2007) Hydrogen peroxide involvement in formation and development of adventitious roots in cucumber. Plant Growth Regul 52:173–180CrossRefGoogle Scholar
  20. Li SW, Xue LG, Xu SJ, An LZ (2009) Hydrogen peroxide acts as a signal molecule in the adventitious root formation of mung bean seedlings. Environ Exp Bot 65:63–71CrossRefGoogle Scholar
  21. Lichtenthaler HK (1987) Chlorophylls and carotenoids pigments of photosynthetic biomembranes. Methods Enzymol 148:350–382CrossRefGoogle Scholar
  22. Meloni DA, Oliva MA, Martinez CA, Cambraia J (2003) Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ Exp Bot 49:69–76CrossRefGoogle Scholar
  23. Molassiotis A, Sotiropoulos T, Tanou G, Diamantidis G, Therios I (2006) Boron-induced oxidative damage and antioxidant and nucleolytic responses in shoot tips culture of the apple rootstock EM9 (Malus domestica Borkh). Environ Exp Bot 56:54–62CrossRefGoogle Scholar
  24. Murphy TM, Sung WW, Lin CH (2002) H2O2 treatment induces glutathione accumulation and chilling tolerance in mung bean. Funct Plant Biol 29:1081–1087CrossRefGoogle Scholar
  25. Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880Google Scholar
  26. Nasir Kham M, Manzer HS, Mohammad F, Naeem M, Masroor M, Kham A (2010) Calcium chloride and gibberellic acid protect linseed from NaCl stress by inducing antioxidative defense system and osmoprotectant accumulation. Acta Physiol Plant 32:121–132CrossRefGoogle Scholar
  27. Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Ann Rev Plant Physiol Plant Mol Biol 49:249–279CrossRefGoogle Scholar
  28. Overmyer K, Brosche H, Kangasjarvi J (2003) Reactive oxygen species and hormonal control of cell death. Trends Plant Sci 8:335–342PubMedCrossRefGoogle Scholar
  29. Pastori GM, Foyer CH (2002) Common components, networks, and pathways of cross-tolerance to stress. The central role of redox and abscisic acid-mediated controls. Plant Physiol 129:460–468PubMedCrossRefGoogle Scholar
  30. Prasad TK, Anderson MK, Martin BA, Stewart CR (1994) Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide. Plant Cell 6:65–74PubMedCrossRefGoogle Scholar
  31. Predieri S, Norma MA, Krizek DT (1995) Influence of UV-B radiation on membrane lipid composition and ethylene of evolution in ‘Doyenne d’Hiver’ pear shoots grown in vitro under different photosynthetic photo fluxes. Environ Exp Bot 35:152–260CrossRefGoogle Scholar
  32. Shalata A, Tal M (1998) The effect of salt stress on lipid peroxidation and antioxidants in the leaf of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii. Physiol Plant 104:169–174CrossRefGoogle Scholar
  33. Shalata A, Mittova V, Volokita M, Guy M, Tal M (2001) Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon Pennellii to salt-dependent oxidative stress: the root antioxidative system. Physiol Plant 112:487–494PubMedCrossRefGoogle Scholar
  34. Tonamura B (1978) Test reactions for a stopped flow apparatus regulation of 2, 6-D and potassium ferricynide by l-ascorbic acid. Anal Biochem 84:370–383CrossRefGoogle Scholar
  35. Uchida A, Jagendorf AT, Hibino T, Takabe T (2002) Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice. Plant Sci 163:515–523CrossRefGoogle Scholar
  36. Vaidyanathan H, Sivakumar P, Chakrabarty R, Thomas G (2003) Scavenging of reactive oxygen species in NaCl-stressed rice (Oryza sativa L.) differential response in salt-tolerant and sensitive varieties. Plant Sci 165:1411–1418CrossRefGoogle Scholar
  37. Wang Y, Yang ZM, Zhang QF, Li JL (2009) Enhanced chilling tolerance in Zoysia matrella by pre-treatment with salicylic acid, calcium chloride, hydrogen peroxide or 6-benzylaminopurine. Biol Plant 53(1):179–182CrossRefGoogle Scholar
  38. Wang Y, Li JL, Wang JZ, Li ZK (2010) Exogenous H2O2 improves the chilling tolerance of manilagrass and mascarenegrass by activating the antioxidative system. Plant Growth Regul 61(2):195–204CrossRefGoogle Scholar
  39. Yasar F, Ellialtioglu S, Yildiz K (2008) Effect of salt stress on antioxidant defense systems, lipid peroxidation and chlorophyll content in green bean. Russ J Plant Physiol 55(6):782–786CrossRefGoogle Scholar
  40. Zhang JX, Kirham MB (1994) Osmotic stress-induced changes in activities of superoxide dismutase, catalase and peroxidase in wheat species. Plant Cell Physiol 35:785–791Google Scholar
  41. Zhang X, Zhang L, Dong F, Gao J, Galbraith DW, Song CP (2001) Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba. Plant Physiol 126:1438–1448PubMedCrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010

Authors and Affiliations

  • Jin-Ting Li
    • 1
  • Zong-Bo Qiu
    • 1
  • Xiao-Wei Zhang
    • 1
  • Lin-Song Wang
    • 1
  1. 1.College of Life ScienceHenan Normal UniversityXinxiangPeople’s Republic of China

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