Acta Physiologiae Plantarum

, Volume 32, Issue 5, pp 961–970 | Cite as

Molecular characterization of a cysteine proteinase inhibitor, PgCPI, from Panax ginseng C. A. Meyer

  • Dae-Young Jung
  • Ok Ran Lee
  • Yu-Jin Kim
  • Jung-Hye Lee
  • Rama Krishna Pulla
  • Gayathri Sathiyaraj
  • Ju-Sun Shim
  • Deok-Chun Yang
Original Paper

Abstract

Phytocystatins are plant-derived cysteine proteinase inhibitors implicated in the endogenous regulation of protein turnover and defense mechanisms against insects and pathogens. A cysteine proteinase inhibitor, PgCPI, was isolated and characterized from Panax ginseng. Sequence analysis revealed that the coding cDNA sequence of PgCPI is 609 base pairs in length with a predicted molecular mass of 22.5 kDa. A GenBank BlastX search revealed that the deduced amino acid sequence of PgCPI shares a high-degree homology with cysteine proteinase inhibitors from other plants. In this present study, we analyzed the expression patterns of PgCPI against various abiotic and biotic stresses at different time points using quantitative real time-PCR. Enzymatic activity of PgCPI was also determined against cysteine proteinase. Our results reveal that PgCPI is moderately induced by NaCl, chilling, CuSO4, ABA, and jasmonic acid. However, high light, UV, MeJA, and wounding triggered a significant induction (more than fourfold) of PgCPI within 12-h post-treatment, especially PgCPI prominently accumulated by wounding (24-fold). In addition, increased transcripts of PgCPI were investigated in fungal- and nematode-infected roots. These results suggest that PgCPI is involved in defense responses to biotic and abiotic stresses.

Keywords

Cysteine proteinase Cysteine proteinase inhibitor Panax ginseng Biotic stress Abiotic stress qRT-PCR 

Abbreviation

EST

Expressed sequence tag

CPI

Cysteine proteinase inhibitor

ORF

Open reading frame

qRT-PCR

Quantitative reverse transcription-polymerase chain reaction

ABA

Abscisic acid

MeJA

Methyl jasmonate

UV

Ultraviolet

Notes

Acknowledgment

This study was supported by KGCMVP for Technology Development Program of Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010

Authors and Affiliations

  • Dae-Young Jung
    • 1
  • Ok Ran Lee
    • 1
  • Yu-Jin Kim
    • 1
  • Jung-Hye Lee
    • 1
  • Rama Krishna Pulla
    • 1
  • Gayathri Sathiyaraj
    • 1
  • Ju-Sun Shim
    • 1
  • Deok-Chun Yang
    • 1
    • 2
  1. 1.Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource BankKyung Hee UniversitySuwonSouth Korea
  2. 2.Department of Oriental Medicinal Material and Processing, College of Life ScienceKyung Hee UniversityKiheung Yongin KyunggiSouth Korea

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