Advertisement

Acta Physiologiae Plantarum

, Volume 32, Issue 3, pp 531–539 | Cite as

Molecular cloning and characterization of a jasmonate biosynthetic pathway gene for allene oxide cyclase from Jatropha curcas

  • Bin Liu
  • Wenguo Wang
  • Jihai Gao
  • Fang Chen
  • Shenghua Wang
  • Ying Xu
  • Lin Tang
  • Yongjiong Jia
Original Paper

Abstract

Herein, we cloned a full-length cDNA encoding allene oxide cyclase (AOC, EC 5.3.99.6) that is a key enzyme in jasmonates (JAs) biosynthetic pathway from Jatropha curcas L., an important plant species as its seed is the raw material for biodiesels, named as JcAOC (GenBank accession no. FJ874630). The cDNA was 924 bp in length with a complete open reading frame of 750 bp, which encoded a polypeptide of 250 amino acids including a putative signal peptide of 65 amino acid residues and a mature protein of 185 amino acids with a predicted molecular mass of 20.7 kDa and a isoelectric point of 6.24. Phylogenetic analysis indicated that JcAOC belonged to the AOC superfamily. Semi-quantitative RT-PCR analysis revealed that JcAOC mRNA was expressed in roots, stems, leaves, young seeds, endosperms, and flowers, but that the expression level was highest in leaves and lowest in seeds, and mRNA expression of JcAOC could be induced by salt stress (300 mM NaCl) and low temperature (4°C). Furthermore, the full-length coding region of JcAOC excluding signal peptide sequence was inserted into pET-30a and was successfully expressed in Escherichia coli. Overexpression of JcAOC in E. coli conferred its resistance to salt stress and low temperature.

Keywords

Allene oxide cyclase Expression Jatropha curcas Molecular cloning Stresses 

Notes

Acknowledgments

We thank the reviewers for useful suggestions. This research is supported by grants from “11th Five-Year Plan” to support science and technology project (NO. 2007BAD50B05) and Ministry of education key program (No. 307023).

References

  1. Agrawal GK, Jwa NS, Agrawal SK, Tamogami S, Iwahashi H, Rakwal R (2003) Cloning of novel rice allene oxide cyclase (OsAOC): mRNA expression and comparative analysis with allene oxide synthase (OsAOS) gene provides insight into the transcriptional regulation of octadecanoid pathway biosynthetic genes in rice. Plant Sci 164:979–992CrossRefGoogle Scholar
  2. Agrawal GK, Tamogami S et al (2004) Rice octadecanoid pathway. Biochem Biophys Res Commun 317:1–15CrossRefPubMedGoogle Scholar
  3. Andradea A, Viglioccoa A, Alemanoa S et al (2005) Endogenous jasmonates and octadecanoids in hypersensitive tomato mutants during germination and seedling development in response to abiotic stress. Seed Sci Res 15:309–318CrossRefGoogle Scholar
  4. Bohlmann H, Vignutelli A, Hilpert B et al (1998) Wounding and chemicals induce expression of the Arabidopsis thaliana gene Thi2.1, encoding a fungal defense thionin, via the octadecanoid pathway. FEBS Lett 437:281–286CrossRefPubMedGoogle Scholar
  5. Cenzano A, Abdala G, Hause B (2007) Cytochemical immuno-localization of allene oxide cyclase, a jasmonic acid biosynthetic enzyme, in developing potato stolons. J Plant Physiol 164:1449–1456CrossRefPubMedGoogle Scholar
  6. Delker C, Stenzel I, Hause B, Miersch O, Feussner I, Wasternack C (2006) Jasmonate biosynthesis in Arabidopsis thaliana—enzymes, products, regulation. Plant Biol 8:297–306CrossRefPubMedGoogle Scholar
  7. Farmer EE, Ryan CA (1990) Interplant communication—airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant-leaves. Proc Natl Acad Sci USA 87:7713–7716CrossRefPubMedGoogle Scholar
  8. Fortes AM, Miersch O, Lange PR et al (2005) Expression of allene oxide cyclase and accumulation of jasmonates during organogenic nodule formation from hop (Humulus lupulus var. Nugget) internodes. Plant Cell Physiol 46:1713–1723CrossRefPubMedGoogle Scholar
  9. Hause B, Stenzel I, Miersch O, Maucher H, Kramell R, Ziegler J, Wasternack C (2000) Tissue-specific oxylip in signature of tomato flowers: allene oxide cyclase is highly expressed in distinct flower organs and vascular bundles. Plant J 24:113–126CrossRefPubMedGoogle Scholar
  10. Isayenkov S, Mrosk C, Stenzel I, Strack D, Hause B (2005) Suppression of allene oxide cyclase in hairy roots of Medicago truncatula reduces jasmonate levels and the degree of mycorrhization with Glomus intraradices. Plant Physiol 139:1401–1410CrossRefPubMedGoogle Scholar
  11. Jiang K, Pi Y, Huang Z, Hou R, Zh Zhang, Lin J, Sun X, Tang K (2009) Molecular cloning and mRNA expression profiling of the first specific jasmonate biosynthetic pathway gene allene oxide synthase from Hyoscyamus niger. Russ J Genet 4:430–439CrossRefGoogle Scholar
  12. Lin J, Yan F, Tang L, Chen F (2003) Anti-tumor effects of curcin from seeds of Jatropha cucrcas. Acta Pharmacol Sin 24:241–246PubMedGoogle Scholar
  13. Maucher H, Stenzel I, Miersch O, Stein N, Prasad M, Zierold U et al (2004) The allene oxide cyclase of barley (Hordeum vulgare L.)—cloning and organ-specific expression. Phytochemistry 65:801–811CrossRefPubMedGoogle Scholar
  14. Pauwels L, Morreel K, De Witte E, Lammertyn F, Van MM, Boerjan W, Inze D, Goossens A (2008) Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cells. Proc Natl Acad Sci USA 105:1380–1385CrossRefPubMedGoogle Scholar
  15. Saneoka H, Ishiguro S, Moghaieb REA (2001) Effect of salinity and abscisic acid on accumulation of glycinebetaine and betaine aldehyde dehydrogenase mRNA in Sorghum leaves (Sorghum bicolor). J Plant Physiol 158:853–859CrossRefGoogle Scholar
  16. Schaller F (2001) Enzymes of the biosynthesis of octadecanoid-derived signaling molecules. J Exp Bot 52:11–23CrossRefPubMedGoogle Scholar
  17. Schaller F, Schaller A, Stintzi A (2005) Biosynthesis and metabolism of jasmonates. J Plant Growth Regul 23:179–199Google Scholar
  18. Stenzel I, Hause B, Miersch O, Kurz T, Maucher M, Weichert H, Ziegler J, Feussner I, Wasternack C (2003) Plant Mol Biol 51:895–911CrossRefPubMedGoogle Scholar
  19. Thatcher LF, Anderson JP, Singh KB (2005) Plant defence responses: what have we learnt from Arabidopsis? Funct Plant Biol 32:1–19CrossRefGoogle Scholar
  20. Thomma B, Eggermont K, Penninckx I et al (1998) Separate jasmonate-dependent and salicylate-dependent defense response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens. Proc Natl Acad Sci USA 95:15107–15111CrossRefPubMedGoogle Scholar
  21. Wang H, Huang ZJ, Chen Q et al (2004) Ectopic over expression of tomato JERF3 in tobacco activates downstream gene expression and enhances salt tolerance. Plant Mol Biol 55:183–192CrossRefPubMedGoogle Scholar
  22. Wasternack C (2007) Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development. Ann Bot 100:681–697CrossRefPubMedGoogle Scholar
  23. Wasternack C, Hause B (2002) Jasmonates and octadecanoids: signals in plant stress responses and plant development. Prog Nucl Acid Res 72:165–221CrossRefGoogle Scholar
  24. Yamada A, Saitoh T, Mimura T, Ozeki Y (2002) Expression of mangrove allene oxide cyclase enhances salt tolerance in Escherichia coli, yeast, and tobacco cells. Plant Cell Physiol 43:903–910CrossRefPubMedGoogle Scholar
  25. Yan P, Jiang KJ, Gao Y et al (2009) Allene oxide cyclase from camptotheca acuminata improves tolerance against low temperature and salt stress in tobacco and bacteria. Mol Biotechnol 41:115–122CrossRefGoogle Scholar
  26. Yoshikawa H, Honda C, Kondo S (2007) Effect of low temperature stress on abscisic acid, jasmonates, and polyamines in apples. Plant Growth Regul 52:199–206CrossRefGoogle Scholar
  27. Zhang FL, Niu B, Wang YC, Chen F et al (2008) A novel betaine aldehyde dehydrogenase gene from Jatropha curcas, encoding an enzyme implicated in adaptation to environmental stress. Plant Sci 174:510–518Google Scholar
  28. Ziegler J, Hamberg M, Miersch O, Parthier B (1997) Purification and characterization of allene oxide cyclase from dry corn seeds. Plant Physiol 114:565–573PubMedGoogle Scholar
  29. Ziegler J, Stenzel I, Hause B, Maucher H, Hamberg M, Grimm R, Ganal M, Wasternack C (2000) Molecular cloning of allene oxide cyclase–the enzyme establishing the stereochemistry of octadecanoids and jasmonates. J Biol Chem 275:19132–19138CrossRefPubMedGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010

Authors and Affiliations

  • Bin Liu
    • 1
  • Wenguo Wang
    • 1
  • Jihai Gao
    • 1
  • Fang Chen
    • 1
  • Shenghua Wang
    • 1
  • Ying Xu
    • 1
  • Lin Tang
    • 1
  • Yongjiong Jia
    • 1
  1. 1.College of Life SciencesSichuan UniversityChengduPeople’s Republic of China

Personalised recommendations