Acta Physiologiae Plantarum

, Volume 32, Issue 2, pp 405–409 | Cite as

High frequency in vitro propagation of Isodon wightii (Bentham) H. Hara

  • R. ThirugnanasampandanEmail author
  • G. Mahendran
  • V. Narmatha Bai
Short Communication


A protocol for in vitro propagation of Isodon wightii (Bentham) H. Hara from nodal segments was developed. Multiple shoots were successfully established on half strength MS medium supplemented with 4.4 μM BA. Enhancement of shoot multiplication and elongation was achieved on half strength MS medium supplemented with 4.4 μM BA and 1.4 μM GA3. The regenerated shoots were rooted successfully on half strength MS medium supplemented with 4.9 μM IBA. Acclimatization of in vitro rooted shoots was successful. The in vitro regenerated plants grew well in the greenhouse without any phenotypic changes.


Isodon wightii Nodal explants Multiple shoot Micropropagation 





Gibberellic acid


Indole-3-acetic acid


Indole-3-butyric acid




α-Naphthaleneacetic acid






  1. Begum F, Amin MN, Azad MAK (2002) In vitro rapid clonal propagation of Ocimum basilicum L. Plant Tissue Cult 12:27–35Google Scholar
  2. Fujita E, Node M (1984) Progress in the chemistry of organic natural products. In: Herz W, Grisebach H, Kirby GW, Tamm CH (eds) vol 46. Springer-Verlag, Vienna, pp 77–157Google Scholar
  3. Iram Siddique MA (2008) An improved plant regeneration system and ex vitro acclimatization of Ocimum basilicum L. Acta Physiol Plant 30:493–499CrossRefGoogle Scholar
  4. Liu W, Chilcott CE, Reich R, Hellmann GM (2000) Regeneration of Salvia sclarea via organogenesis. In Vitro Cell Dev Biol Plant 36:201–206Google Scholar
  5. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays for tobacco tissue cultures. Physiol Plantarum 15:473–497CrossRefGoogle Scholar
  6. Pattnaik S, Chand PK (1996) In vitro propagation of the medicinal herbs Ocimum americanum L. syn. O. canum Sims. (Hoary basil) and O. sanctum L. Holy basil). Plant Cell Rep 15:846–850CrossRefGoogle Scholar
  7. Prakash S, Van Staden J (2007) Micropropagation of Hoslundia opposita Vahl—a valuable medicinal plant. S Afr J Bot 73:60–63CrossRefGoogle Scholar
  8. Sahoo Y, Pattnaik SK, Chand PK (1997) In vitro clonal propagation of a medicinal plant Ocimum basilicum L. (sweet basil) by axillary shoot proliferation. In Vitro Cell Dev Biol Plant 33:293–296CrossRefGoogle Scholar
  9. Skala E, Wysokinska H (2004) In vitro regeneration of Salvia nemorosa from shoot tips and leaf explants. In Vitro Cell Dev Biol 40:596–602CrossRefGoogle Scholar
  10. Sun HD, Huang SX, Han QB (2006) Diterpenoids from Isodon species and their biological activities. Nat Prod Rep 23:673–698CrossRefPubMedGoogle Scholar
  11. Thirugnanasampandan R, Jayakumar R, Narmatha Bai V, Martin E, Rajendra Prasad KJ (2008) Antiacetylcholinesterase and antioxidant ent-kaurene diterpenoid, melissoidesin from Isodon wightii (Bentham) H. Hara. Nat Prod Res 22:687–694CrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2009

Authors and Affiliations

  • R. Thirugnanasampandan
    • 1
    • 2
    Email author
  • G. Mahendran
    • 1
  • V. Narmatha Bai
    • 1
  1. 1.Department of Botany, School of Life SciencesBharathiar UniversityCoimbatoreIndia
  2. 2.Kongunadu Arts and Science CollegeCoimbatoreIndia

Personalised recommendations