Acta Physiologiae Plantarum

, 31:1129 | Cite as

Drought stress effects on Rubisco in wheat: changes in the Rubisco large subunit

  • Klimentina DemirevskaEmail author
  • Diana Zasheva
  • Rumen Dimitrov
  • Lyudmila Simova-Stoilova
  • Maria Stamenova
  • Urs Feller
Original Paper


The leaf protein pattern from drought-tolerant and drought-sensitive wheat varieties subjected to severe soil drought but with the possibility for recover from stress was studied by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The spots representing Rubisco large subunit (RLS) were identified using polyclonal antibodies against Rubisco and immunoblotting. Some qualitative and quantitative differences in the 2D-PAGE protein map of wheat varieties were revealed under drought conditions. Three days recovery of wheat plants were not enough for restoring RLS quantity to the level of controls after 7 days drought, especially in the drought-sensitive variety Miziya. There are contradictory data in the literature concerning increased or diminished RLS level in drought stressed plants. A comparison of RLS after SDS-PAGE and 2D-PAGE was made. The revealed protein pattern depended on the presence or absence of protease inhibitors in the extraction buffer, on the procedure of extraction, and on the degree of stress.


2D-PAGE Drought stress Triticum aestivum L. Rubisco LS SDS-PAGE 



Bovine serum albumin


2-Dimensional polyacrylamide gel electrophoresis




Dry weight


Ethylenediaminetetraacetic acid


Electrolyte leakage


Fresh weight


Isoelectric focusing




Molecular mass


Phenylmethanesulfonyl fluoride


Ribulose-1,5-bisphosphate carboxylase/oxygenase


Rubisco large subunit


Rubisco small subunit


Sodium dodecyl sulfate polyacrylamide gel electrophoresis


Shoot length


Trichloracetic acid


Turgor weight


Water deficit



This study was supported by grants from Swiss National Science Foundation, SCOPES (project DILPA) and from Ministry of Education and Science of Republic Bulgaria (project CC 1503). The authors are grateful to Dr I. Stancheva for her advices in growing wheat plants in soil and to B. Juperlieva-Mateeva and A. Kostadinova for their excellent technical assistance.


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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2009

Authors and Affiliations

  • Klimentina Demirevska
    • 1
    Email author
  • Diana Zasheva
    • 2
  • Rumen Dimitrov
    • 2
  • Lyudmila Simova-Stoilova
    • 1
  • Maria Stamenova
    • 2
  • Urs Feller
    • 3
  1. 1.Institute of Plant PhysiologyBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Institute of Biology and Immunology of ReproductionBulgarian Academy of SciencesSofiaBulgaria
  3. 3.Institute of Plant SciencesUniversity of Bern3013Switzerland

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