In vitro rapid multiplication and propagation of Cardiospermum halicacabum L. through axillary bud culture
- 329 Downloads
A simple, rapid and efficient protocol for micropropagation of Cardiospermum halicacabum via axillary bud multiplication has been successfully developed. The organogenic competence of nodal segments was investigated on Murashige and Skoog (MS) medium supplemented with different concentrations of benzyladenine (BA), kinetin (Kn), thidiazuron (TDZ) and 2-isopentenyladenine (2-iP). Multiple shoots differentiated directly without callus mediation within 4 weeks when explants were cultured on a medium fortified with cytokinins. The maximum number of shoots (14.83 ± 0.52) was developed on a medium supplemented with 0.3 μM TDZ. Such proliferating shoots when subcultured onto MS media devoid of TDZ gave the highest rate of shoot multiplication (35.66 ± 1.00) by the end of fourth subculture passage. Elongated shoots were rooted on 1/3 MS medium augmented with 0.5 μM IAA. The plantlets thus obtained were successfully hardened and transferred to greenhouse.
KeywordsCardiospermum halicacabum Micropropagation Nodal segment Thidiazuron Sapindaceae
α-Naphthalene acetic acid
Murashige and Skoog’s medium
Authors gratefully acknowledge the Department of Science and Technology, Govt. of India, New Delhi, for providing research support under DST-FIST Program.
- Anonymous (1992) The wealth of India. Raw Materials, Publications and Information Directorate. CSIR, N Delhi 3:269–271Google Scholar
- Bajaj YPS, Furmanowa M, Olszowska O (1988) Biotechnology of micropropagation of medicinal and aromatic plants. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry medicinal plants. I, vol 4. Springer, Berlin, pp 60–103Google Scholar
- Kirtikar KR, Basu BD (1935) Indian medicinal plants. M/s Bishen Singh Mahendrapal, New Delhi, pp 267–268Google Scholar
- Parliman BJ, Evans PT, Rupert EA (1982) Tissue culture of single rhizome explants of Dionaea muscipula Ellis ex. L., the venus fly trap for rapid asexual propagation. HortScience 107:305–310Google Scholar
- Siddique I, Anis M (2006) Thidiazuron induced high frequency shoot bud formation and plant regeneration from cotyledonary node explants of Capsicum annuum L. Indian J Biotechnol 5:303–308Google Scholar
- Varier PS (1993) Indian medicinal plants, vol I. Orient Longman, MadrasGoogle Scholar