Acta Physiologiae Plantarum

, Volume 31, Issue 1, pp 133–138 | Cite as

In vitro rapid multiplication and propagation of Cardiospermum halicacabum L. through axillary bud culture

  • Anushi A. Jahan
  • Mohammad Anis
Original Paper


A simple, rapid and efficient protocol for micropropagation of Cardiospermum halicacabum via axillary bud multiplication has been successfully developed. The organogenic competence of nodal segments was investigated on Murashige and Skoog (MS) medium supplemented with different concentrations of benzyladenine (BA), kinetin (Kn), thidiazuron (TDZ) and 2-isopentenyladenine (2-iP). Multiple shoots differentiated directly without callus mediation within 4 weeks when explants were cultured on a medium fortified with cytokinins. The maximum number of shoots (14.83 ± 0.52) was developed on a medium supplemented with 0.3 μM TDZ. Such proliferating shoots when subcultured onto MS media devoid of TDZ gave the highest rate of shoot multiplication (35.66 ± 1.00) by the end of fourth subculture passage. Elongated shoots were rooted on 1/3 MS medium augmented with 0.5 μM IAA. The plantlets thus obtained were successfully hardened and transferred to greenhouse.


Cardiospermum halicacabum Micropropagation Nodal segment Thidiazuron Sapindaceae 





Indole-3-acetic acid


Indole-3-butyric acid




α-Naphthalene acetic acid


6-Furfurylaminopurine (kinetin)


Murashige and Skoog’s medium





Authors gratefully acknowledge the Department of Science and Technology, Govt. of India, New Delhi, for providing research support under DST-FIST Program.


  1. Ahmad N, Anis M (2007) Rapid clonal propagation of a woody tree, Vitex negundo L. through axillary shoots proliferation. Agrofor Syst 71:195–200. doi: 10.1007/s10457-007-9078-1 CrossRefGoogle Scholar
  2. Ahmad N, Siddique I, Anis M (2006) Improved plant regeneration in Capsicum annuum L. from nodal segments. Biol Plant 50:701–704. doi: 10.1007/s10535-006-0110-5 CrossRefGoogle Scholar
  3. Anonymous (1992) The wealth of India. Raw Materials, Publications and Information Directorate. CSIR, N Delhi 3:269–271Google Scholar
  4. Babber S, Mittal K, Ahlawat R, Varghese TM (2001) Micropropagation of Cardiospermum halicacabum. Biol Plant 44:603–606. doi: 10.1023/A:1013763208472 CrossRefGoogle Scholar
  5. Bajaj YPS, Furmanowa M, Olszowska O (1988) Biotechnology of micropropagation of medicinal and aromatic plants. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry medicinal plants. I, vol 4. Springer, Berlin, pp 60–103Google Scholar
  6. Borthakur M, Hazarika J, Singh RS (1999) A protocol for micropropagation of Alpinia galanga. Plant Cell Tissue Organ Cult 55:231–233. doi: 10.1023/A:1006265424378 CrossRefGoogle Scholar
  7. Faisal M, Ahmad N, Anis M (2005) Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tissue Organ Cult 80:187–190. doi: 10.1007/s11240-004-0567-x CrossRefGoogle Scholar
  8. Faisal M, Siddique I, Anis M (2006) An efficient plant regeneration system for Mucuna pruriens L. using cotyledonary node explants. In Vitro Cell Dev Biol Plant 42:59–64. doi: 10.1079/IVP2005717 CrossRefGoogle Scholar
  9. Fracaro F, Echeverrigaray S (2001) Micropropagation of Cunila galioides, a popular medicinal plant of south Brazil. Plant Cell Tissue Organ Cult 64:1–4. doi: 10.1023/A:1010626200045 CrossRefGoogle Scholar
  10. Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119. doi: 10.1007/BF01983223 CrossRefGoogle Scholar
  11. Joshi M, Dhar U (2003) In vitro propagation of Saussurea aobvallata (D.C.) Edgew—an endangered ethnoreligeous medicinal herb of Himalaya. Plant Cell Rep 21:933–939. doi: 10.1007/s00299-003-0601-1 PubMedCrossRefGoogle Scholar
  12. Kirtikar KR, Basu BD (1935) Indian medicinal plants. M/s Bishen Singh Mahendrapal, New Delhi, pp 267–268Google Scholar
  13. Koroch AR Jr, Juliani HR, Juliani HR, Trippi VS (1997) Micrporopagation and acclimatization of Hedeoma multiflorum. Plant Cell Tissue Organ Cult 48:213–217. doi: 10.1023/A:1005804121136 CrossRefGoogle Scholar
  14. Liu CZ, Murch SJ, EL-Demerdash M, Saxena PK (2003) Regeneration of Egyptian medicinal plant Artemesia judaica L. Plant Cell Rep 21:525–530PubMedGoogle Scholar
  15. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays for tobacco tissue culture. Physiol Plant 15:473–497. doi: 10.1111/j.1399-3054.1962.tb08052.x CrossRefGoogle Scholar
  16. Parliman BJ, Evans PT, Rupert EA (1982) Tissue culture of single rhizome explants of Dionaea muscipula Ellis ex. L., the venus fly trap for rapid asexual propagation. HortScience 107:305–310Google Scholar
  17. Pattnaik SK, Chand PK (1996) In vitro proagation of medicinal herbs Ocimum americanum L. syn. Ocimum canum Sims (hoary basil) and Ocimum sanctum (holy basil). Plant Cell Rep 15:846–850. doi: 10.1007/BF00233154 CrossRefGoogle Scholar
  18. Rout GR, Saxena C, Samantray S, Das P (1999) Rapid clonal propagation of Plumbago zeylanica L. Plant Growth Regul 2:1–4. doi: 10.1023/A:1006146713143 CrossRefGoogle Scholar
  19. Siddique I, Anis M (2006) Thidiazuron induced high frequency shoot bud formation and plant regeneration from cotyledonary node explants of Capsicum annuum L. Indian J Biotechnol 5:303–308Google Scholar
  20. Siddique I, Anis M (2007) In vitro shoot multiplication and plantlet regeneration from nodal explant of Cassia angustifolia (Vahl.). Acta Physiol Plant 29:233–239. doi: 10.1007/s11738-007-0029-2 CrossRefGoogle Scholar
  21. Siddique I, Anis M (2008) An improved plant regeneration system and ex vitro acclimatization of Ocimum basilicum L. Acta Physiol Plant 30:493–499. doi: 10.1007/s11738-008-0146-6 CrossRefGoogle Scholar
  22. Thomas TD, Maseena EA (2006) Callus induction and plant regeneration in Cardiospermum halicacabum L.—a medicinally important plant. Sci Horti 108:332–336. doi: 10.1016/j.scienta.2006.02.008 CrossRefGoogle Scholar
  23. Varier PS (1993) Indian medicinal plants, vol I. Orient Longman, MadrasGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2008

Authors and Affiliations

  1. 1.Plant Biotechnology Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia

Personalised recommendations