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Acta Physiologiae Plantarum

, Volume 30, Issue 6, pp 825–832 | Cite as

Peroxidase and catalase changes during in vitro adventitious shoot organogenesis from hypocotyls of Albizia odoratissima L.f. (Benth)

  • V. RajeswariEmail author
  • Kailash Paliwal
Original Paper

Abstract

Hypocotyls of Albizia odoratissima cultured on shoot induction medium (MS medium with 7.5 μM BAP and 0.5 μM NAA) showed adventitious shoot organogenesis under light with 16 h photoperiod. Similar cultures under total darkness produced non-morphogenic calli. The changes in the specific peroxidase and catalase activity, total protein content and acidic isoperoxidase pattern were compared between the culture showing shoot organogenesis and culture producing non-morphogenic calli. It was found that in vitro shoot bud differentiation is accompanied by an increase of the specific activities of peroxidase and catalase in culture kept under light. In parallel with the above changes the total protein content reached to the maximum level and also a new isoperoxidase (P10) expressed on the 21st day in cultures kept under light. Conversely, culture producing non-morphogenic calli underwent a reverse change in specific peroxidase activity. This change in antioxidant enzyme activities corresponds to the histological observation of shoot bud differentiation in cultures kept under light.

Keywords

Shoot organogenesis Peroxidase Catalase Acidic isoperoxidase 

Abbreviations

BAP

6-Benzyl amino purine

NAA

α Naphthalene acetic acid

AOS

Active oxygen species

Notes

Acknowledgements

The financial assistance provided by the Council of Scientific and Industrial Research (CSIR), Government of India to V. Rajeswari is gratefully acknowledged. The authors are also thankful to anonymous reviewers for their constructive comments on an earlier version of the manuscript.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2008

Authors and Affiliations

  1. 1.Department of Plant Sciences, School of Biological SciencesMadurai Kamaraj UniversityMaduraiIndia

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