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Acta Physiologiae Plantarum

, Volume 30, Issue 5, pp 657–662 | Cite as

Mitochondrial RNA editing of F0-ATPase subunit 9 gene (atp9) transcripts of Yunnan purple rice cytoplasmic male sterile line and its maintainer line

  • Lei Wei
  • Zhi-Xiang Yan
  • Yi DingEmail author
Original Paper

Abstract

On the base of construction of a new type of cytoplasmic male sterile (CMS) line ZidaoA, we analyzed the editing of transcripts of mitochondrial ATP synthase subunit 9 gene (atp9) from CMS line and its maintainer line. With PCR, RT-PCR, and direct sequencing, complete nucleotide sequences were determined for the mitochondrial atp9 gene and its cDNA from two lines of purple rice type rice: CMS line Ying xiang A and its maintainer line Ying xiang B. The atp9 transcript of Ying xiang A was shown to have no editing sites and the transcript of Ying xiang B was shown to have two editing sites with changes affecting the amino acid sequence of the protein product. The editing of the atp9 transcript from Ying xiang B was found to change an arginine codon into a termination codon, shortening the protein of Ying xiang B to the “standard” size. And the Ying xiang A transcript, which has no termination codon, cannot be translated to a normal protein. The results demonstrate the important role of RNA editing in the production of the functional ATP9 subunit and suggest that RNA editing could be likely associated with cytoplasmic male sterility.

Keywords

atp9 Cytoplasmic male sterile (CMS) RNA editing Ying xiang A Ying xiang B 

Abbreviations

BLAST

Basic local alignment search tool

CMS

Cytoplasmic male sterile

Notes

Acknowledgments

We thank Dr. Josephine Richardson for her critical reading of the manuscript. We gratefully acknowledge Dr. Sun Qingping (Wuhan University) for his kind help with reverse transcription. This study was supported in part by the National Science Foundation of China (No. 30571143).

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2008

Authors and Affiliations

  1. 1.Key Laboratory of MOE for Plant Developmental Biology, College of Life SciencesWuhan UniversityWuhanPeople’s Republic of China

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