Advertisement

Acta Physiologiae Plantarum

, Volume 30, Issue 5, pp 629–637 | Cite as

Accumulation of lead in root cells of Pisum sativum

  • Arleta Małecka
  • Aneta Piechalak
  • Iwona Morkunas
  • Barbara Tomaszewska
Original Paper

Abstract

The ever-increasing environmental pollution necessitates organisms to develop specific defense systems in order to survive and function effectively. Lead is taken up by plants mainly through roots and over 96% are accumulated there.Pea plants were cultivated hydroponically for 4 days with 0.1, 0.5 and 1 mM Pb(NO3)2. Uptake of lead ions from nutrient solution and accumulation in root stems and leaves during 96-h cultivation was estimated. The root tip cells were observed with transmission electron microscope to analyse their ultrastructure and lead localization. Pb was accumulated in the cell wall, cell membrane, vacuoles, mitochondria and peroxisomes. The fractions of mitochondria and peroxisomes were isolated from pea roots purified by means Percoll gradient, and were observed by means of electron microscope with the attachment for X-ray microanalysis. Visible deposits containing Pb were observed in both cell organelles.

Keywords

Energy dispersive X-ray microanalysis (EDAX) Pb accumulation Pisum sativum 

Notes

Acknowledgments

This work was supported by the State Committee for Scientific Research (KBN), grant no. 2 P04G 069 26.

References

  1. Antosiewicz D (2005) Study of calcium-dependent lead-tolerance on plants differing in their level of Ca-deficiency tolerance. Environ Pollut 134:23–34PubMedCrossRefGoogle Scholar
  2. Antosiewicz D, Wierzbicka MM (1999) Localization of lead in Alium cepa L. cells by electron microscopy. J Microsc 195:139–146PubMedCrossRefGoogle Scholar
  3. Baralkiewicz D, Kozka M, Gramowska H, Tomaszewska B, Wasinkiewicz K (2004) Determination of lead in plants in controlling phytoremediation processes using slurry sampling electrothermal atomic absorption spectrometry. Int J Environ Anal Chem 84:901–908CrossRefGoogle Scholar
  4. Chen XC, Wang YP, Lin Q, Shi JY, Wu WX, Chen YX (2005) Biosorption of copper (II) and zinc (II) from aqueous solution by Pseudomonas putia CZ1. Colloids Surf B 46:101–107CrossRefGoogle Scholar
  5. Colangelo EP, Gueriont ML (2006) Put the metal to the petal: metal uptake and transport throughout plants. Curr Opin Plant Biol 9(3):322–330PubMedCrossRefGoogle Scholar
  6. Eboh L, Mepba HD, Ekpo MB (2006) Heavy metal contaminants and processing effects on the composition, storage stability and fatty acid profiles of five common commercially available fish species in Oron Local Government, Nigeria. Food Chem 97:490–497CrossRefGoogle Scholar
  7. Hall JL (2001) Cellular mechanisms for heavy metal detoxification and tolerance. J Exp Bot 53:1–11CrossRefGoogle Scholar
  8. Jarvis MD, Leung DWM (2001) Chelated lead transport in Chamaecytisus proliferus (L.f.) link ssp. proliferus var. palmensis (H. Christ): an ultrastructural study. Plant Sci 161:433–441CrossRefGoogle Scholar
  9. Jian-Yi S, Ming-Yan J, Jian-Feng W, Nai-Tao Z, Liang-Jian F, Ming-Qi L, Lin P (2006) Effect of zinc of biochemical parameters and changes in related gene expression assessed by cDNA microarrays in pituitary of growing rats. Nutrion 22:187–196CrossRefGoogle Scholar
  10. Leopold I, Günther D (1997) Investigation of binding properties of heavy-metal-peptide complexes in plant cell cultures using HPLC-ICP-MS. J Anal Chem 359:364–370CrossRefGoogle Scholar
  11. Liu D, Jiang W, Liu C, Xin C, Hou W (2000) Uptake and accumulation of lead by roots, hypocotyls and shoots of Indian mustard [Brassica juncea (L.)]. Bioresour Technol 71:273–277CrossRefGoogle Scholar
  12. Marmiroli M, Antonioli G, Maestri E, Marmiroli N (2005) Evidence of the involvement of plant ligno-cellulosic structure in the sequestration of Pb: an X-ray spectroscopy-based analysis. Environ Pollut 134:217–227PubMedCrossRefGoogle Scholar
  13. Perez de Mora A, Burgos P, Madejon E, Cabrera F, Jaeckel P, Schloter M (2006) Microbial community structure and function in a soil contaminated by heavy metals: effects of plant growth and different amendments. Soil Biol Biochem 38:327–341Google Scholar
  14. Piechalak A, Tomaszewska B, Baralkiewicz D (2003) Enhancing phytoremediative ability of Pisum sativum by EDTA application. Phytochemistry 62:1239–1251CrossRefGoogle Scholar
  15. Piechalak A, Tomaszewska B, Baralkiewicz D, Malecka A (2002) Accumulation and detoxification of lead ions in legumes. Phytochemistry 60:153–167PubMedCrossRefGoogle Scholar
  16. Polec-Pawlak K, Ruzik R, Lipec E, Ciurzynska M, Gawronska H. (2007) Investigation of Pb(II) binding to pectin in Arabidopsis thaliana. J Anal Atom Spectrom 22:968–972CrossRefGoogle Scholar
  17. Robinson DG, Hillmer S (1990) Endocytosis in plants. Physiol Plant 79:96–104CrossRefGoogle Scholar
  18. Samardakiewicz S, Wozny A (2000) The distribution of lead in duckweed (Lemna mimor L.) root tip. Plant Soil 226:107–111CrossRefGoogle Scholar
  19. Schűtzendűbel A, Polle A (2002). Plant responses to abiotic stresses: heavy metal- induced oxidative stress and protection by mycorrhization. J Exp Bot 53:1351–1365PubMedCrossRefGoogle Scholar
  20. Seregin IV, Pekhov VM, Ivanow VB (2002) Plasmolysis as a tool to reveal lead localization in the apoplast of root cells. Russ J Plant Physiol 49:283–285CrossRefGoogle Scholar
  21. Singh RP, Tripathi RD, Sinha SK, Maheshwari R, Srivastava HS (1997) Response of higher plants to lead contaminated environment. Chemosphere 34:2467–2493PubMedCrossRefGoogle Scholar
  22. Tung G, Temple PJ (1996) Uptake and localization of lead in corn (Zea mays L.) seedlings, a study by histochemical and electron microscopy. Sci Total Environ 188:71–85PubMedCrossRefGoogle Scholar
  23. Wierzbicka M (1987) Lead accumulation and its translocation barriers in roots of Allium cepa L.—autoradiographic and ultrastructural studies. Plant Cell Environ 10:17–26CrossRefGoogle Scholar
  24. Wierzbicka M (1995) How lead loses its toxicity to plants. Acta Soc Bot Pol 64:81–90Google Scholar
  25. Wierzbicka M (1999) Comparison of lead tolerance in Allium cepa with other plant species. Environ Pollut 104:41–52CrossRefGoogle Scholar
  26. Wojcik M, Tukiendorf A (2003) Response of wild type of Arabidopsis thaliana to copper stress. Biol Plant 46:79–84CrossRefGoogle Scholar
  27. Wozny A, Krzeslowska M, Tomaszewska B, Wozny A (1995) In: Wozny A (ed) Lead in plant cells. Sorus, Poznan, pp 21–110 (in polish)Google Scholar
  28. Zenk MH (1996) Heavy metal detoxification in higher plants—a review. Gene 179:21–30PubMedCrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2008

Authors and Affiliations

  • Arleta Małecka
    • 1
  • Aneta Piechalak
    • 1
  • Iwona Morkunas
    • 2
  • Barbara Tomaszewska
    • 1
  1. 1.Department of BiochemistryUniversity of Adam MickiewiczPoznanPoland
  2. 2.Department of Plant PhysiologyThe August Cieszkowski Agricultural UniversityPoznanPoland

Personalised recommendations