Acta Physiologiae Plantarum

, Volume 30, Issue 3, pp 339–343 | Cite as

The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants

  • Kong Sik Shin
  • Hosakatte Niranjana Murthy
  • Jeong Wook Heo
  • Eun Joo Hahn
  • Kee Yoeup PaekEmail author
Original Paper


The influence of light quality on growth and development of in vitro grown Doritaenopsis hort. (Orchidaceae) plants was investigated. Growth parameters like leaf and root fresh/dry mass and leaf area were highest with plants grown under red plus blue light emitting diodes (LEDs). Leaf length was greater with the plants grown under red LED. Carbohydrate (starch, sucrose, glucose and fructose) and leaf pigment (chlorophylls and carotenoids) biosynthesis of the plants was significantly increased in plants grown under red plus blue LEDs compared to red or blue LED and fluorescent light treatments. This study suggests that the production of quality Doritaenopsis plants is possible by culturing the plants in vitro under a mixture of blue plus red light sources.


Doritaenopsis In vitro Light emitting diodes Light quality Chlorophylls Carotenoids 



This work was supported by Korea Science and Engineering Foundation (KOSEF) through Research Center for the Development of Advanced Horticultural Technology at Chungbuk National University, Cheongju, 361–763, Korea.


  1. Brown CS, Schuerger AC, Sager JC (1995) Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red lighting. J Am Soc Hortic Sci 120:808–813PubMedGoogle Scholar
  2. Chaplin MF (1986) Monosaccharides. In: Chaplin MF, Kennedy JF (eds) Carbohydrate analysis a practical approach. IRL Press, Oxford, pp 1–36Google Scholar
  3. Huges KW (1981) In vitro ecology: exogenous factors effecting growth and morphogenesis in plant culture system. Environ Exp Bot 21:281–288CrossRefGoogle Scholar
  4. Kozai T, Smith MAL (1995) Environmental control in the plant tissue culture: General introduction and overview. In: Aitken-Christie J, Kozai T, Smith MAL (eds) Automation and environmental control in plant tissue culture. Kluwer, Dordrecht, pp 301–418Google Scholar
  5. Lichtenthaler HK (1987) Chlorophyll and carotenoids: pigments of photosynthetic biomembranes. Method Enzymol 48:350–382CrossRefGoogle Scholar
  6. Park SY, Murthy HN, Paek KY (2002) Rapid propagation of Phalaenopsis from floral stalk derived leaves. In Vitro Cell Dev Biol Plant 38:168–172CrossRefGoogle Scholar
  7. Schuerger AC, Brown CS, Stryjewski EC (1997) Anatomical features of pepper plants (Capsicum annuum L.) grown under red light-emitting diodes supplemented with blue or far-red light. Ann Bot 79:273–282PubMedCrossRefGoogle Scholar
  8. Shin KS, Chakrabarthy D, Paek KY (2002) Sprouting rate, changes of carbohydrate content and related enzymes during cold treatment of lily bulblets regenerated in vitro. Sci Hort 96:195–204CrossRefGoogle Scholar
  9. Tanaka M, Takamura T, Watanabe H, Endo M, Yanagi T, Okamoto K (1998) In vitro growth of Cymbidium plantlets cultured under super bright red and blue light emitting diodes (LEDs). J Hortic Sci Biotech 73:39–44Google Scholar
  10. Tennessen DJ, Singsaas EL, Sharkey TD (1994) Light emitting diodes as a light source for photosynthesis research. Photosynth Res 39:85–92CrossRefGoogle Scholar
  11. Tibbitts TW, Morgan DC, Warrington JJ (1983) Growth of lettuce, spinach, mustard and wheat plants under four combinations of high-pressure sodium, metal halide and tungsten halogen lamps at equal PPFD. J Am Hortic Sci 108:622–630Google Scholar
  12. Tripathy BC, Brown CS (1995) Root-shoot interaction in greening of wheat seedlings grown under red light. Plant Physiol 107:407–511PubMedGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2008

Authors and Affiliations

  • Kong Sik Shin
    • 1
  • Hosakatte Niranjana Murthy
    • 2
    • 3
  • Jeong Wook Heo
    • 4
  • Eun Joo Hahn
    • 2
  • Kee Yoeup Paek
    • 2
    Email author
  1. 1.Biosafety DivisionNational Institute of Agricultural Biotechnology, RDASuwonRepublic of Korea
  2. 2.Research Center for the Development of Advanced Horticultural TechnologyChungbuk National UniversityCheongjuRepublic of Korea
  3. 3.Department of BotanyKarnatak UniversityDharwadIndia
  4. 4.Division of Bio-Production Fundamental EngineeringNational Institute of Agricultural Engineering, RDASuwonRepublic of Korea

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