Advertisement

Acta Physiologiae Plantarum

, Volume 30, Issue 3, pp 395–399 | Cite as

Petal: a reliable explant for direct bulblet regeneration of endangered wild populations of Fritillaria imperialis L.

  • Manijeh Mohammadi-Dehcheshmeh
  • Ahmad Khalighi
  • Roohangiz Naderi
  • Manoochehr Sardari
  • Esmaeil Ebrahimie
Short Communication

Abstract

Wild populations of Fritillaria imperialis L. are facing extinction and need urgent conservation. This paper presents an efficient system for in vitro direct bulblet regeneration of these populations by petal culturing of flower buds. Petals at different developmental stages, green-closed flower bud (before nectar secretion) and red-closed flower bud (beginning of nectar secretion), were used as explants, and the effects of various proportions of cytokinin to auxin on direct bulblet regeneration pathway were evaluated. More explants switched on direct regeneration pathway in combination of auxins (0.6 mg l−1 NAA + 0.4 mg l−1 IAA) with higher level of cytokinin (1 mg l−1 BAP). In contrast, auxins (0.6 mg l−1 NAA + 0.4 mg l−1 IAA) with lower level of cytokinin (0.1 mg l−1 BAP) produced more bulblets per regenerated explant. In green-closed flower bud stage, direct bulblets regenerated from the end of petal where it was connected to the receptacle, while nectar secretion site was the place of bulblet formation in red-closed flower bud stage. In addition, genotype-dependency of direct bulblet regeneration pathway was investigated by using two different wild populations of Fritillaria imperialis. This plant regeneration procedure was applicable to different Fritillaria genotypes and regenerated bulblets were normal.

Keywords

Direct regeneration Endangered plant Fritillaria Genotype-dependency Nectar Plant conservation 

Abbreviations

BAP

6-Benzylaminopurine

IAA

Indol-3-acetic acid

NAA

α-Naphthaleneacetic acid

PGR

Plant growth regulator

References

  1. Barth FG (1991) Insects and flowers: the biology of a partnership. MA Biederman-Thorson, Princeton University, GermanyGoogle Scholar
  2. De Hertogh A, Le Nard M (1993) The physiology of flower bulbs. Elsevier, AmsterdamGoogle Scholar
  3. Ebrahimie E, Mohammadi-Dehcheshmeh M, Sardari M (2006a) Fritillaria species are at the risk of extinction in Iran: study on effective factors and necessity of international attention. Hortscience 41:1002Google Scholar
  4. Ebrahimie E, Habashi AA, Mohammadie-Dehcheshmeh M, Ghannadha M, Ghareyazie B, Yazdi-Amadi B (2006b) Direct shoot regeneration from mature embryo as a rapid and genotype-independent pathway in tissue culture of heterogeneous diverse sets of cumin (Cuminum cyminum L.) genotypes. In Vitro Cell Dev Biol Plant 42:455–460Google Scholar
  5. Ebrahimie E, Naghavi M.R, Hosseinzadeh A, Behamta M.R, Mohammadi-Dehcheshmeh M, Sarrafi A, Spangenberg G (2007) Induction and comparison of different in vitro morphogenesis pathways using embryo of cumin (Cuminum cyminum L.) as a model material. Plant Cell Tissue Organ Cult 90:293–311CrossRefGoogle Scholar
  6. Eudes E, Acharya S, Laroche A, Selinger LB, Cheng KJ (2003) A novel method to induce direct somatic embryogenesis, secondary embryogenesis and regeneration of fertile green cereal plants. Plant Cell Tissue Organ Cult 73:147–153CrossRefGoogle Scholar
  7. Fay M (1992) Conservation of rare and endangered plants using in vitro methods. In Vitro Cell Dev Biol Plant 28:1–4Google Scholar
  8. Feito I, Rodiguez A, Centeno ML, Sanchez-Tames R, Fernandez B (1994) Effect of the physical nature of the culture of medium on the metabolism of benzyladenine and endogenous cytokinins in Actinidia deliciosa tissues cultured in vitro. Physiol Plant 91:449–453CrossRefGoogle Scholar
  9. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158PubMedCrossRefGoogle Scholar
  10. Gibson S (2005) Control of plant development and gene expression by sugar signaling. Plant Biol 8:93–102Google Scholar
  11. Mohammadi-dehcheshmeh M, Khalighi A, Naderi R, Ebrahimie E, Sardari M (2007) Indirect somatic embryogenesis from petal explant of endangered wild population of Fritillaria imperialis. Pak J Biol Sci 10:1875–1879CrossRefPubMedGoogle Scholar
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth of and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479CrossRefGoogle Scholar
  13. Paek KY, Murthy HN (2002) High frequency of bulblet regeneration from bulb scale sections of Fritillaria thunbergii. Plant Cell Tissue Organ Cult 68:247–252CrossRefGoogle Scholar
  14. Pintos B, Martin JP, Centeno ML, Villalobos N, Guerra H, Martin L (2002) Endogenous cytokinin levels in embryogenic and non-embryogenic calli of Medicago arborea L. Plant Sci 163:955–960CrossRefGoogle Scholar
  15. Witomska M, Lukaszewska A (1997) Bulblet regeneration in vitro from different explants of Fritillaria imperialis. Acta Hortic 430:331–338Google Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2007

Authors and Affiliations

  • Manijeh Mohammadi-Dehcheshmeh
    • 1
  • Ahmad Khalighi
    • 1
  • Roohangiz Naderi
    • 1
  • Manoochehr Sardari
    • 2
  • Esmaeil Ebrahimie
    • 3
  1. 1.Department of Horticultural Sciences, Faculty of AgricultureUniversity of TehranKarajIran
  2. 2.Agricultural and Natural Resources Research CenterShahrekordIran
  3. 3.Department of Crop Production and Plant Breeding, Faculty of AgricultureShiraz UniversityShirazIran

Personalised recommendations