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Alterations in root lipid peroxidation and antioxidative responses in two rice cultivars under NaCl-salinity stress

  • M. H. KhanEmail author
  • S. K. Panda
Original Paper

Abstract

The comparative alterations of short term NaCl stress and recovery on growth, water relations, ionic composition, lipid peroxidation and antioxidants in roots of two rice cultivars differing in salt tolerance were studied. Exposed for 24 h to increasing (50, 100 and 150 mmol l−1) concentrations of NaCl, roots of 12D Oryza sativa L. cv. Lunishree and cv. Begunbitchi decreased in fresh weight, dry weight and relative water content. Increased Na+ and decreased K+ ion were determined at increasing NaCl concentrations. Both peroxide content and lipid peroxidation measured in terms of MDA level increased and the ratio was higher in Begunbitchi compared to Lunishree. Recovered roots showed lower peroxide and MDA content. Ascorbate and glutathione contents increased in the stressed and recovered roots of Lunishree, but decreased in Begunbitchi with increasing NaCl concentrations. Although SOD, CAT and GR activities decreased in the stressed roots, CAT activity also increased in recovered roots of both the cultivars. The POX activity increased in stressed and recovered roots of both Lunishree and Begunbitchi. Higher free radicals scavenging capacity and more efficient protection mechanism of Lunishree against salt stress, as revealed by the lower level of lipid peroxidation and improved plant water status as well as activities of some of the antioxidants, suggest that significant cultivar differences in response to salt stress in rice are closely related to differences in the activities of antioxidants and ion content. Another possible conclusion is that improved tolerance to salt stress may be accomplished by increased capacity of antioxidative system.

Keywords

Antioxidative enzymes Ascorbate Glutathione Malondialdehyde NaCl-salinity Oryza sativa L. 

Abbreviations

AOS

active oxygen species

CAT

catalase

GR

glutathione reductase

MDA

malondialdehyde

NBT

nitrobluetetrazolium

POX

peroxidase

RWC

relative water content

TCA

trichloroacetic acid

TBA

thiobarbituric acid

SOD

superoxide dismutase

Wi

initial fresh weight

Wd

dry weight

Wf

turgid weight

Notes

Acknowledgment

M. H. K acknowledges the Council of Scientific & Industrial Research (CSIR), New Delhi for granting Senior Research Fellowship (Award No. 9/747/2/2003 EMR-8 & 9/747(2)/2003 EMR-1 dated 26-05-2005). Rice seeds received from Central Rice Research Institute, Cuttack, Orissa and Regional Rice Research Station (Assam Agricultural University), Karimganj, Assam are gratefully acknowledged.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2007

Authors and Affiliations

  1. 1.Plant Biochemistry Laboratory, School of Life SciencesAssam UniversitySilcharIndia

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