Acta Physiologiae Plantarum

, Volume 28, Issue 1, pp 3–8 | Cite as

Yew (Taxus x media Rehd.) cell suspension cultures as a source of taxanes

  • Matjaž Hren
  • Špela Baebler
  • Marjana Camloh
  • Maja Kova
  • Maja Ravnikar
  • Jana Žel
Article
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Accepted:

Abstract

Natural resources of paclitaxel, an effective anticancer compound, were threatened with extinction soon after the discovery of this valuable substance. Cell suspension cultures derived from different Taxus species have rapidly become an alternative source of paclitaxel and other taxanes. In this paper we provide some insight into cell growth characteristics in cell suspension culture of Taxus x media cv. Hicksii, with emphasis on the effects of jasmonic acid (JA) on taxane production in cell lines with different initial taxane content. Additionally cell growth characteristics of two cell lines was followed during cultivation of cell suspension culture of Taxus x media cv. Hicksii. Packed cell volume (PCV) was shown to be a reliable and efficient alternative for measuring cell growth instead of fresh and dry weight. The initial total taxane content was screened in a number of cell lines, followed by observing the effect of JA on cell mass and total taxane production of selected lines. We showed a great variability in initial taxane content in different cell lines, which decreased during cell suspension maintenance. JA was shown to inhibit cell growth and increase total taxane production (14 to 106 fold).

Key words

tissue culture jasmonic acid taxol Taxus x media cv. Hicksii 

List of abbreviations

2,4-D

2,4-dichlorophenoxyacetic acid

CIEIA

competitive inhibition enzyme immunoassay system

FDA

fluorescein diacetate

JA

jasmonic acid

MeJA

methyl jasmonate

PCV

packed cell volume

SE

standard error

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Copyright information

© Department of Plant Physiology 2006

Authors and Affiliations

  • Matjaž Hren
    • 1
  • Špela Baebler
    • 1
  • Marjana Camloh
    • 1
  • Maja Kova
    • 1
  • Maja Ravnikar
    • 1
  • Jana Žel
    • 1
  1. 1.Department of Plant Physiology and BiotechnologyNational Institute of BiologyLjubljanaSlovenia

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