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Acta Physiologiae Plantarum

, Volume 29, Issue 2, pp 143–149 | Cite as

The influence of apigenin on membrane and action potential in the liverwort Conocephalum conicum

  • Bożena Pawlikowska-PawlęgaEmail author
  • Elżbieta Król
  • Kazimierz Trębacz
  • Antoni Gawron
Original Paper

Abstract

Apigenin (4′,5,7-trixydroxyflavone) is a member of the family of plant flavonoids considered to prevent a number of human diseases, for instance cancer development. It displays a lot of activities and part of its beneficial effects could come from its affinity to the cellular membranes. In the present study we used the liverwort Conocephalum conicum, a model plant in electrophysiological study. Intracellular microelectrode measurements were carried out to examine the effects of apigenin alone and in combination with verapamil on the resting and action potentials. The application of apigenin caused an increase of action potential amplitudes. An increase even by 110–131% with respect to the control was observed. Little increase was also found in the membrane potentials in apigenin treated plants. Verapamil, the known calcium channel inhibitor, caused gradual decline of AP amplitudes. When apigenin was used simultaneously with verapamil, still high APs were observed. Duration of action potentials amplitudes measuerd at a half of the amplitude decreased in either apigenin or apigenin and verapamil treated plants to 56–62% of the control. It is concluded that apigenin strongly affects the membranes and prevents inhibitory effect of verapamil probably interacting with calcium channel protein.

Keywords

Conocephalum conicum Action potentials Membrane potential Flavonoids Apigenin Verapamil Calcium channels 

List of abbreviations

AP

Action potential

DMSO

Dimethyl sulfoxide

MP

Membrane potential

MES

(2-[N-morpholino]ethanesulfonic acid)

Tris

(Tris[hydroxymethyl]aminomethane)

References

  1. Agullo G, Gamet-Payrastre L, Manenti S, Viala C, Remesy C, Chap H, Payrastre B (1997) Relationship between flavonoid structure and inhibition of phosphatidylinositol-3-kinase: a comparison with tyrosine kinase and protein kinase C inhibition. Biochem Pharmacol 53:1649–1657PubMedCrossRefGoogle Scholar
  2. Arora A, Byrem TM, Nair MG, Strasburg GM (2000) Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids. Arch Biochem Biophys 1:102–109CrossRefGoogle Scholar
  3. Boyong L, Robinson DH, Birt DF (1997) Evaluation of properties of apigenin and [G-3H] apigenin and analytic method development. Pharm Sci 6:721–725Google Scholar
  4. Caci E, Folli Ch, Zegarra-Moran O, Ma T, Sprigsteel MF, Sammelson RE, Nantz MH, Kurth MJ, Verkman AS, Galietta LJV (2003) CFTR activation in human bronchial epithelial cells by novel benzoflavone and benzimidazolone compounds. Am Physiol Lung Cell Mol Physiol 285:L180–L188Google Scholar
  5. Chaumontet C, Bex V, Gaillard-Sanchez I, Seillan-heberden Ch, Suschetet M, Martel P (1994) Apigenin and tangeretin enhance gap junctional intercellular communication in rat liver epithelial cells. Carcinog 10:2325–2330CrossRefGoogle Scholar
  6. Das A, Wang IH, Lien EJ (1994) Carcinogenicity, mutagenicity and cancer preventing activities flavonoids: a structure–system activity relationship (SSAR) analysis. Prog Drug Res 42:133–166PubMedGoogle Scholar
  7. Duarte J, Vizcaino EP, Utrilla P, Jimenez J, Tamargo J, Zarzuel A (1993) Vasodilatory effects of flavonoids in rat aortic smooth muscle–structure–activity relationships. Gen Pharmacol 4:857–862Google Scholar
  8. Dziubińska H, Trębacz K, Zawadzki T (1989) Electrical activity of the liverwort Conocephalum conicum: The effect of excitation on the rate of respiration in the liverwort Conocephalum conicum. Physiol Plant 75:417–423CrossRefGoogle Scholar
  9. Ferriola PC, Cody V, Middleton E (1989) Protein kinase C inhibition by plant flavonoids. Kinetic mechanisms and structure–activity relationships. Biochem Pharmacol 38:1617–1624PubMedCrossRefGoogle Scholar
  10. Formica JV, Regelson W (1995) Review of the biology of quercetin and related bioflavonoids. Fd Chem Toxic 33:1061–1080CrossRefGoogle Scholar
  11. Frangne N, Eggmann T, Koblischke C, Weissenbock G, Martinoia E, Klein M (2002) Flavone glucoside uptake into barley mesophyll and arbidopsis cell culture vacuoles. Energization occurs by H+ antiport and ATP-binding cassette-type mechanisms. Plant Physiol 128:726–733PubMedCrossRefGoogle Scholar
  12. Gupta S, Afaq F, Mukhtar H (2002) Involvement of nuclear factor-kappa B, Bax and Bcl-2 in induction of cell cycle arrest and apoptosis by apigenin in human prostate carcinoma cells. Oncogene 21:3727–3738PubMedCrossRefGoogle Scholar
  13. Illek B, Fischer H (1998) Flavonoids stimulate Cl conductance of human airway epithelium in vitro and in vivo. Am J Physiol 275:L902–L910PubMedGoogle Scholar
  14. Klein M, Martinoia E, Weissenbock G (1997) Transport of Lucifer yellow CH into plant vacuoles—evidence for direct energization of a sulphonated substance and implications for the design of new molecular probes. FEBS Lett 420:86–92PubMedCrossRefGoogle Scholar
  15. Kuo ML, Yang NCh (1995) Reversion of v-H-ras- transformed NIH 3T3 cells by apigenin through inhibiting mitogen activated protein kinase and its downstream oncogenes. Biochem Biophys Res Commun 3:767–775CrossRefGoogle Scholar
  16. Lenne-Gouverneur A.F., Lobstein A., Haan-Archipoff G., Duportail G., Anton R., Kuhry JG (1999) Interaction of the monomeric and dimeric flavones apigenin and amentoflavone with the plasma membrane of L929 cells; fluorescence study. Mol Membr Biol 16:157–165PubMedCrossRefGoogle Scholar
  17. Lim M, McKenzie K, Floyd AD, Kwon E, Zeitlin PL (2004) Modulation of ΔF508 cystic fibrosis transmembrane regulator trafficking and function with 4-phenylbutyrate and flavonoids. Am J Respir Cell Mol Biol 31:351–357PubMedCrossRefGoogle Scholar
  18. Morales MA, Lozoya X (1994) Calcium-antagonist effects of quercetin on aortic smooth muscle. Planta Med 60:313–317PubMedCrossRefGoogle Scholar
  19. Pawlikowska-Pawlęga B, Trębacz K, Król E, Gawron A (2000) Effects of quercetin and verapamil on membrane potential in the liverwort Conocephalum conicum. Acta Physiol Plant 1:61–68CrossRefGoogle Scholar
  20. Pawlikowska-Pawlęga B, Gruszecki WI, Misiak LE, Gawron A (2003) The study of the quercetin action on human erythrocyte membranes. Biochem Pharmacol 66:605–612PubMedCrossRefGoogle Scholar
  21. Qui YL, Cho YR, Cox JC, Palmer JD (1998) The gain of three mitochondrial introns identifies liverwort as the earliest land plants. Nature 394:671–6674CrossRefGoogle Scholar
  22. Shukla S, Gupta S (2004) Molecular mechanisms for apigenin-induced cell-cycle arrest and apoptosis of hormone refractory human prostate carcinoma DU145 cells. Mol Carcinog 39:114–126PubMedCrossRefGoogle Scholar
  23. Takahashi L, Sert MA, Kelmer-Bracht AM, Bracht A, Ishii-Iwamoto EI (1998) Effects of rutin and quercetin on mitochondrial metabolism and on ATP levels in germinarting tissues of Glycine max. Plant Physiol Biochem 36:495–501CrossRefGoogle Scholar
  24. Trębacz K, Zawadzki T (1985) Light-triggered action potentials in the liverwort Conocephalum conicum. Physiol Plant 64:482–486CrossRefGoogle Scholar
  25. Trębacz K, Tarnecki R, Zawadzki T (1989) The effect of ionic channel inhibitors and factors modifying metabolism on the excitability of the liverwort Conocephalum conicum. Physiol Plant 75:24–30CrossRefGoogle Scholar
  26. Trębacz K, Simonis W, Schönknecht G (1994) Cytoplasmic Ca2+, K+, Cl -, and NO3- activities in the liverwort Conocephalum conicum L. at rest and during action potentials. Plant Physiol 106:1073–1084PubMedGoogle Scholar
  27. Trębacz K, Simonis W, Schönknecht G (1997) Effects of anion channel inhibitors on light-induced potential changes in the liverwort Conocephalum conicum. Plant Cell Physiol 5:550–557Google Scholar
  28. Wang HK, Xia Y, Yang ZY, Natschke SL, Lee KH (1998) Recent advances in the discovery and development of flavonoids and their analogues as antitumour and anti-HIV agents. Adv Exp Med Biol 439:191–225PubMedGoogle Scholar
  29. Wang IK, Lin-Shiau SY, Lin JK (1999) Induction of apoptosis by apigenin and related flavonoids through cytochrome c release and activation of caspase-9 and caspase-3 in leukaemia HL-60 cells. Eur J Cancer 10:1517–1525CrossRefGoogle Scholar
  30. Wójtowicz K, Pawlikowska-Pawlęga B, Gawron A, Misiak LE, Gruszecki WI (1996) Modifying effect of quercetin on the lipid membrane. Folia Histochem Cytobiol 34:48–50Google Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2007

Authors and Affiliations

  • Bożena Pawlikowska-Pawlęga
    • 1
    Email author
  • Elżbieta Król
    • 2
  • Kazimierz Trębacz
    • 2
  • Antoni Gawron
    • 1
  1. 1.Department of Comparative Anatomy and AnthropologyMaria Curie-Sklodowska UniversityLublinPoland
  2. 2.Department of BiophysicsMaria Curie-Sklodowska UniversityLublinPoland

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