Acta Physiologiae Plantarum

, Volume 29, Issue 2, pp 121–126 | Cite as

Podophyllotoxin content in Podophyllum hexandrum Royle plants of known age of seed origin and grown at a lower altitude

  • Hemant Pandey
  • Shyamal K. Nandi
  • Anil Kumar
  • Uma T. Palni
  • Lok Man S. Palni
Original Paper

Abstract

Podophyllum hexandrum Royle, an important alpine herb, and a source of the highly valued aryltetralin-type lignan, podophyllotoxin, has been subjected to heavy collection from the wild due to ever increasing demand. The present study deals with an attempt to bring this plant under cultivation at a relatively lower altitude and to evaluate (1) various growth parameters including above and below ground biomass accumulation, net assimilation rate and relative growth rate, etc., and (2) podophyllotoxin content from the resulting above and below ground biomass in seed raised plants of a known age series of 1–5 years. The podophyllotoxin content was estimated on the basis of HPLC analyses. The levels were found to increase with the plant age and the maximum amount was found in 5-year old plants. This study demonstrates that (1) seeds can be conveniently used for raising healthy propagules in easily approachable locations at a relatively lower altitude, and that the plants can be maintained in such sites over long periods, and (2) this approach of “conservation through cultivation” can be suggested as an effective tool for the management of this “critically endangered status” species.

Keywords

Alpine Cultivation Podophyllum hexandrum Podophyllotoxin 

Notes

Acknowledgments

Financial support to Hemant Pandey from a project funded by the Department of Biotechnology, Government of India is duly acknowledged. The Ministry of Environment and Forests, Government of India and the Director of the Institute are also thanked for providing facilities.

References

  1. Aung LH (1990) Growth substances and environment on the development of vegetative storage organs. In: Purohit SS (ed) Hormonal regulation of plant growth and development. Agro Botanical Publishers, Bikaner, pp 91–106Google Scholar
  2. Beutner KR, von Krogh G (1990) Current status of podophyllotoxin for the treatment of genital warts. Semin Dermatol 9:148–151PubMedGoogle Scholar
  3. Bhadula SK, Singh A, Lata H, Kuniyal CP, Purohit AN (1996) Genetic resources of Podophyllum hexandrum Royle, an endangered medicinal species from Garhwal Himalaya, India. Plant Genet Resour Newsl 106:26–29Google Scholar
  4. Baronov A (1966) Recent advances in our knowledge of the morphology, cultivation and uses of ginseng (Panex ginseng A Meyer). Econ Bot 20:403–406Google Scholar
  5. Canel C, Moreas RM, Dyan FE, Ferreira D (2000) Molecules of interest: Podophyllotoxin. Phytochemistry 54:115–120PubMedCrossRefGoogle Scholar
  6. Canel C, Dayan FE, Ganzera M, Khan IA, Rimando A, Burandt CL, Moraes RM (2001) High yield of podophyllotoxin from leaves of Podophyllum peltatum by in situ conversion of podophyllotoxin 4-O-β-d glucopyranoside. Planta Medica 67:97–99PubMedCrossRefGoogle Scholar
  7. Chauhan RS, Nautiyal MC (2005) Commercial viability of an endangered medicinal herb Nardostachys jatamansi at three different agroclimatic zones. Curr Sci 89:1481–1488Google Scholar
  8. Evans GC (1972) The quantitative analysis of plant growth. Black Well, OxfordGoogle Scholar
  9. Fay DA, Ziegler HW (1985) Botanical source differentiation of Podophyllum resin by high performance liquid chromatography. J Liq Chromatogr 8:1501–1506CrossRefGoogle Scholar
  10. Nadeem M, Palni LMS, Purohit AN, Pandey H, Nandi SK (2000) Propagation and conservation of Podophyllum hexandrum Royle: an important medicinal herb. Biol Conser 92:121–129CrossRefGoogle Scholar
  11. Nadeem M (2001) Mass propagation and conservation strategies for two medicinally important plants of the Himalayan region. PhD thesis, Kumaun University, NainitalGoogle Scholar
  12. Nautiyal MC (1986) Physiology of reproduction in two Aconitum species. PhD thesis H. N. B. Garhwal University, SrinagarGoogle Scholar
  13. Nautiyal BP, Prakash V, Chauhan RS, Purohit H, Nautiyal MC (2001) Assesment of germinability, productivity and cost benefit analysis of Picrorhiza kurrooa cultivated at lower altitude. Cur Sci 81:579–585Google Scholar
  14. Nigam KB (1984) Ashwagandha cultivation. Ind Hortic 28:39–41Google Scholar
  15. Pandey H, Nandi SK, Chandra B, Nadeem M, Palni LMS (2001) GA3 induced flowering in Podophyllum hexandrum Royle: a rare alpine medicinal herb. Acta Physiol Plant 23:467–474CrossRefGoogle Scholar
  16. Pandey H (2002) Studies in relation to cotyledonary senescence and tissue culture of medicinally important alpine herbs. PhD thesis, Kumaun University, NainitalGoogle Scholar
  17. Purohit MC, Bahuguna R, Maithani UC, Purohit AN, Rawat AN, Rawat MSM (1999) Variation in podophylloresin and podophyllotoxin content in different populations of Podophyllum hexandrum. Cur Sci 77:1078–1080Google Scholar
  18. Samant SS, Dhar U, Palni LMS (1998) Medicinal plants of Himalaya: diversity distribution and potential values. Himvikas, Gyanodaya Prakashan, Nainital, p 163Google Scholar
  19. Sharma P, Sett R (2001) Micropropagation of Indian ginseng (Panex pseudoginseng Wall): a proposition to save an endangered commercial and medicinal forest plant. J Human Eco 12:201–205Google Scholar
  20. Singh A (1992) Ecophysiology of Podophyllum hexandrum Royle. PhD thesis, H. N. B. Garhwal University, SrinagarGoogle Scholar
  21. Snedecor GW, Cochran WG (1967) Statistical methods. Oxford and IBH, New Delhi, p 593Google Scholar
  22. Stoltz LP, Snyder JC (1985) Embryo growth and germination of American ginseng seed in response to stratification temperature. Hort Sci 20:261–262Google Scholar
  23. Van Uden W, Pras N, Visser JF, Malingre ThM (1989) Detection and identification of podophyllotoxin produced by cell cultures derived from Podophyllum hexandrum Royle. Plant Cell Rep 8:165–168CrossRefGoogle Scholar
  24. Varshney A, Dhawan V, Srivastava PS (2001) Ginseng: wonder drug of the world. In: Khan IA, Khanum A (eds) Role of biotechnology in medicinal and aromatic plants. Ukaaz Publication, India pp 26–41Google Scholar
  25. Whitehead FH, Myerscough PJ (1962) Growth analysis of plants. The ratio of mean relative growth rate to mean relative rate of leaf area increase. New Phytol 61:314–321CrossRefGoogle Scholar
  26. Woodward FI, Piggot CD (1975) The climatic control of the altitudinal distribution of Sedum rosea (L.) Scop and S. telephinum L. 1. Field observations. New Phytol 74:323–334CrossRefGoogle Scholar
  27. Woodward FI (1979) The differential temperature response of the growth of certain plant species from different altitudes. I. Growth analysis of Phleum alpinum L., P. bertolonii DC, Sesleria albicanas Kit.and Dactylis glomerata L. New Phytol 82:385–395CrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2007

Authors and Affiliations

  • Hemant Pandey
    • 1
  • Shyamal K. Nandi
    • 1
  • Anil Kumar
    • 1
    • 2
  • Uma T. Palni
    • 3
  • Lok Man S. Palni
    • 1
    • 4
  1. 1.Environmental Physiology and BiotechnologyG.B. Pant Institute of Himalayan Environment and DevelopmentAlmoraIndia
  2. 2.TIFAC Core Building, Thapar Institute of Engineering and TechnologyPatialaIndia
  3. 3.Botany DepartmentKumaun UniversityNainitalIndia
  4. 4.State Biotechnology ProgrammeGovernment of UttaranchalU.S. NagarIndia

Personalised recommendations