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Acta Physiologiae Plantarum

, Volume 27, Issue 2, pp 205–212 | Cite as

Effect of hypoxia on photosynthetic activity and antioxidative response in gametophores of Mnium undulatum

  • Andrzej Rzepka
  • Jan Krupa
  • Ireneusz lesak
Article

Abstract

The effects of hypoxia caused by complete submerging of Mnium undulatum gametophores in water, on their photosynthetic activity and the activity of two antioxidative enzymes: superoxide dismutase (SOD) and catalase (CAT) were investigated. The net photosynthesis was strongly inhibited throughout the experiment, and the strong drop in the maximum quantum yield of the PSII (Fv/Fm) was also observed. Three classes of SOD: MnSOD, FeSOD, Cu/ZnSOD and three isoforms of Cu/ZnSOD were identified. A significant decrease in activity of MnSOD, FeSOD and one Cu/ZnSOD isoform was observed after 24 and 48 h of hypoxia. FeSOD activity decreased already after 1 h of submerging in water and its activity remained at the low level during whole period of the experiment. CAT activity was also strongly inhibited in response to hypoxia stress. The obtained results suggest relationships between photosynthetic activity and antioxidative system in M. undulatum gametophores under oxygen deficiency stress.

Key words

catalase chlorophyll a fluorescence hypoxia Mnium undulatum oxidative stress photosynthesis superoxide dismutase 

List of abbreviations

BSA

bovine serum albumin

CAT

catalase

DTT

dithiothreitol

EDTA

ethylenediamine tetraacetic acid

EGTA

ethyleneglycol-bis(beta-aminoethylether)-N,N′-tetracet ic acid

Fm

maximum chlorophyll a fluorescence yield

Fo

minimum chlorophyll a fluorescence yield

Fv

the difference between F m and F o

Fv/Fm

maximum quantum yield of PSII

NBT

nitroblue tetrazolium salt

PAGE

polyacrylamide gel electrophoresis

PAR

photosynthetically active radiation

Pn

net photosynthesis intensity

PSII

photosystem II

ROS

reactive oxygen species

SOD

superoxide dismutase

Tricine

N-[tris-(hydroxymethyl)methyl]glycine

Tris

tris(hydroxymethyl)aminomethane

TEMED

N,N,N′,N′-tetramethylethylenediamine

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Copyright information

© Department of Plant Physiology 2005

Authors and Affiliations

  • Andrzej Rzepka
    • 1
  • Jan Krupa
    • 1
  • Ireneusz lesak
    • 2
  1. 1.Department of Plant Physiology, Institute of BiologyPedagogical AcademyKrakówPoland
  2. 2.Institute of Plant PhysiologyPolish Academy of SciencesKrakówPoland

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