Acta Physiologiae Plantarum

, Volume 25, Issue 1, pp 97–104 | Cite as

Changes of pH in Petunia hybrida (Hort.) styles induced by pollination and influence of proton pump and ion channels on its regulation

  • Maria Filek
  • Maria Wędzony
  • Elżbieta Bednarska


Ionselective microelectrode method was used to study changes of pH in transmitting tissue of style in Petunia hybrida (Hort.). Effect of pollination and pollen tube growth were examined. Subsequently solutions of ions and various stimulators or blockers of ion channels were applied on pollinated styles to examine the possible role of ion channels in pH stabilisation. It was confirmed in the present study that: (1) there is a pH gradient in the transmitting tissue of a petunia unpollinated style with the stigma region being more acidic; (2) pollination causes further acidification of transmitting tissue: (3) the gradient of pH first vanishes at 24 h after pollination then is reversed up to 72 h after pollination; (4) active transport of ions plays an important role in pH regulation in transmitting tissue. The presented results confirm the role of pH changes and Ca2+ as a mediator in controlling proton influx into the apoplast of the transmitting tissue during pollen tube growth.

Key words

Petunia hybrida pH pollination transmitting tissue 


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Copyright information

© Department of Plant Physiology 2003

Authors and Affiliations

  • Maria Filek
    • 1
  • Maria Wędzony
    • 1
  • Elżbieta Bednarska
    • 2
  1. 1.Department of Plant PhysiologyPolish Academy of SciencesKrakówPoland
  2. 2.Institute of Biology and Environment Protection, Department of Experimental BiologyPomeranian Academy of EducationSlupskPoland

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