Changes of pH in Petunia hybrida (Hort.) styles induced by pollination and influence of proton pump and ion channels on its regulation
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Ionselective microelectrode method was used to study changes of pH in transmitting tissue of style in Petunia hybrida (Hort.). Effect of pollination and pollen tube growth were examined. Subsequently solutions of ions and various stimulators or blockers of ion channels were applied on pollinated styles to examine the possible role of ion channels in pH stabilisation. It was confirmed in the present study that: (1) there is a pH gradient in the transmitting tissue of a petunia unpollinated style with the stigma region being more acidic; (2) pollination causes further acidification of transmitting tissue: (3) the gradient of pH first vanishes at 24 h after pollination then is reversed up to 72 h after pollination; (4) active transport of ions plays an important role in pH regulation in transmitting tissue. The presented results confirm the role of pH changes and Ca2+ as a mediator in controlling proton influx into the apoplast of the transmitting tissue during pollen tube growth.
Key wordsPetunia hybrida pH pollination transmitting tissue
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- Allen N.S., Bennett M.N., Cox D.N., Shipley A., Ehrhardt D.W., Long S.R. 1994. Effects of nod factors on alfalfa root hair Ca2+ and H+ currents and on cytoskeletal behaviour. In: M. Daniels, (Ed.) Advances in Molecular Genetics, vol. 3 Kluwer Acad., Dordrecht, The Netherlands, pp. 107–113.Google Scholar
- Bednarska E., Butowt R. 1995. Calcium in pollenpistil interaction in Petunia hybrida Hort. III. Localization of Ca2+ ions and Ca2+-ATPase in pollinated pistil. Folia Hist. Cytobiol. 33: 125–132.Google Scholar
- Guern J., Mathieu Y., Thomine S., Jouanneau J.P., Beloeil J.C. 1992. Plant cell counteract cytoplasmic pH changes but likely use these pH changes as secondary messengers in signal perception. Curr. Top. Plant Biochem. Physiol. 11: 249–269.Google Scholar
- Lenartowska M., Bednarska E., Butowt R. 1997. Ca2+ in the pistil of Petunia hybrida Hort. during growth of pollen tube — cytochemical and radiographic studies. Acta Biol. Cracov. 39: 79–89Google Scholar
- Pierson E.S., Miller D.D., Callaham D.A., Shipley A.M., Rivers B.A., Cresti M., Hepler PK. 1994. Pollen tube growth is coupled to the extracellular calcium ion flux and the intracellular calcium gradient: effect of BAPTA-type buffere and hypertonic media. Plant Cell 6: 1815–1828.PubMedCrossRefGoogle Scholar
- Wędzony M., Filek M. 1996. Changes of electric potential in wheat pistils induced by pollination. Acta Soc. Bot. Pol. 65: 97–100.Google Scholar
- Wędzony M., Filek M. 1998. Changes of electric potential in pistil of Petunia hybrida Hort. and Brassica napus L. during pollination. Acta Physiol. Plant. 20: 291–297.Google Scholar