Advertisement

Acta Physiologiae Plantarum

, Volume 25, Issue 1, pp 97–104 | Cite as

Changes of pH in Petunia hybrida (Hort.) styles induced by pollination and influence of proton pump and ion channels on its regulation

  • Maria Filek
  • Maria Wędzony
  • Elżbieta Bednarska
Article
  • 53 Downloads

Abstract

Ionselective microelectrode method was used to study changes of pH in transmitting tissue of style in Petunia hybrida (Hort.). Effect of pollination and pollen tube growth were examined. Subsequently solutions of ions and various stimulators or blockers of ion channels were applied on pollinated styles to examine the possible role of ion channels in pH stabilisation. It was confirmed in the present study that: (1) there is a pH gradient in the transmitting tissue of a petunia unpollinated style with the stigma region being more acidic; (2) pollination causes further acidification of transmitting tissue: (3) the gradient of pH first vanishes at 24 h after pollination then is reversed up to 72 h after pollination; (4) active transport of ions plays an important role in pH regulation in transmitting tissue. The presented results confirm the role of pH changes and Ca2+ as a mediator in controlling proton influx into the apoplast of the transmitting tissue during pollen tube growth.

Key words

Petunia hybrida pH pollination transmitting tissue 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allen N.S., Bennett M.N., Cox D.N., Shipley A., Ehrhardt D.W., Long S.R. 1994. Effects of nod factors on alfalfa root hair Ca2+ and H+ currents and on cytoskeletal behaviour. In: M. Daniels, (Ed.) Advances in Molecular Genetics, vol. 3 Kluwer Acad., Dordrecht, The Netherlands, pp. 107–113.Google Scholar
  2. Bednarska E., Butowt R. 1995. Calcium in pollenpistil interaction in Petunia hybrida Hort. III. Localization of Ca2+ ions and Ca2+-ATPase in pollinated pistil. Folia Hist. Cytobiol. 33: 125–132.Google Scholar
  3. Beffagna N., Romai G., Meraviglia G., Pallini S. 1997. Effects of abscisic acid and cytoplasmic pH on potassium and chloride efflux in Arabidopsis thaliana seedlings. Plant Cell Physiol. 38: 503–510.PubMedGoogle Scholar
  4. De Nisi P., Dell’Orto M., Pirovano L., Zocchi G., 1999. Calcium-dependent phosphorylation regulates the plasma-membrane H+-ATPase activity of maize (Zea mays L.) roots. Planta. 209: 187–194.PubMedCrossRefGoogle Scholar
  5. Felle H. 1988. Auxin causes oscillations of cytosolic free calcium and pH in Zea mays coleoptiles. Planta. 174: 495–499.CrossRefGoogle Scholar
  6. Felle H.H. 1994. The H+/Cl symporter in root-hair cells of Sinapis alba. Plant Physiol. 106: 1131–1136.PubMedGoogle Scholar
  7. Felle H.H. 1998. The apoplastic pH of the Zea mays root cortex as measured with pH-sensitive microelectrodes: aspects of regulation. J. Exp. Bot. 39: 987–995.CrossRefGoogle Scholar
  8. Felle H. Bertl A. 1986. The fabrication of H+-sensitive liquid-membrane micro-electrodes for use in plant cells. J. Exp. Bot. 37: 1416–1428.CrossRefGoogle Scholar
  9. Felle H.H., Kondorosi E., Kondorosi A., Schultze M. 1996. Rapid alkalization in alfalfa roots in response to rhizobal lipochitooligasaccharide signals. Plant J. 10: 295–301.CrossRefGoogle Scholar
  10. Guern J., Mathieu Y., Thomine S., Jouanneau J.P., Beloeil J.C. 1992. Plant cell counteract cytoplasmic pH changes but likely use these pH changes as secondary messengers in signal perception. Curr. Top. Plant Biochem. Physiol. 11: 249–269.Google Scholar
  11. Lenartowska M., Bednarska E., Butowt R. 1997. Ca2+ in the pistil of Petunia hybrida Hort. during growth of pollen tube — cytochemical and radiographic studies. Acta Biol. Cracov. 39: 79–89Google Scholar
  12. Lenartowska M., Rodriguez-Garcia M., Bednarska E. 2001. Immunocytochemical localization of esterified and unesterified pectins in unpollinated and pollinated styles of Petunia hybrida Hort. Planta. 213: 182–191.PubMedCrossRefGoogle Scholar
  13. Malayev A., Nelson D.J. 1995. Extracellular pH modulates the Ca2+ current activated by depletion of intracellular Ca2+ stores in human macrophages. J. Membr. Biol. 146: 101–111.PubMedGoogle Scholar
  14. Malmström S., Ckerlund H.E., Askerlund P. 2000. Regulatory role of the N terminus of the vacuolar calcium-ATPase in cauliflower. Plant Physiol. 122: 517–526.PubMedCrossRefGoogle Scholar
  15. Marre E. 1979. Fusicoccin: a tool in plant physiology. Ann. Rev. Plant Physiol. 30: 273–288.CrossRefGoogle Scholar
  16. Messerli M., Robinson K.R. 1997. Tip-localized Ca2+ pulses are coincident with peak pulsative growth rates in pollen tubes of Lilium longiflorum. J. Cell Sci. 110: 1269–1278.PubMedGoogle Scholar
  17. Pierson E.S., Miller D.D., Callaham D.A., Shipley A.M., Rivers B.A., Cresti M., Hepler PK. 1994. Pollen tube growth is coupled to the extracellular calcium ion flux and the intracellular calcium gradient: effect of BAPTA-type buffere and hypertonic media. Plant Cell 6: 1815–1828.PubMedCrossRefGoogle Scholar
  18. Pierson E.S., Miller D.D., Callaham D.A., van Aken J., Hackett G., Hepler P.K. 1996. Tip-localized calcium entry fluctuates during pollen tube growth. Dev. Biol. 174: 160–173.PubMedCrossRefGoogle Scholar
  19. Scott A.C., Allen N.S. 1999. Changes in cytosolic pH within Arabodopsis root columella cells play a kay role in the early signalling pathway for root gravitropism. Plant Physiol. 121: 1291–1298.PubMedCrossRefGoogle Scholar
  20. Shabala S.N., Newman I.A, Morris J., 1997. Oscillation in H+ and Ca2+ ion efux around the elongation region of corn roots and effects of external pH. Plant Physiol. 113: 111–118.PubMedGoogle Scholar
  21. Swanson J.S., Jones R.L. 1996. Gibberelic acid induces vacuolar acidification in Barley aleurone. Plant Cell. 8: 2211–2221.PubMedCrossRefGoogle Scholar
  22. Wędzony M., Filek M. 1996. Changes of electric potential in wheat pistils induced by pollination. Acta Soc. Bot. Pol. 65: 97–100.Google Scholar
  23. Wędzony M., Filek M. 1998. Changes of electric potential in pistil of Petunia hybrida Hort. and Brassica napus L. during pollination. Acta Physiol. Plant. 20: 291–297.Google Scholar
  24. Ylstra B., Garrido D., Busscher J., van Tunen AJ. 1998. Hexose transport in growing petunia pollen tubes and characterization of a pollen-specific, putative monosaccharide transporter. Plant Physiol. 118: 297–304.PubMedCrossRefGoogle Scholar
  25. Zimmermann S., Ehrhardt T., Plesch G., Muller-Rober B. 1999. Ion changes in plant signalling. Cell. Mol. Life Sci. 55: 183–203.CrossRefGoogle Scholar

Copyright information

© Department of Plant Physiology 2003

Authors and Affiliations

  • Maria Filek
    • 1
  • Maria Wędzony
    • 1
  • Elżbieta Bednarska
    • 2
  1. 1.Department of Plant PhysiologyPolish Academy of SciencesKrakówPoland
  2. 2.Institute of Biology and Environment Protection, Department of Experimental BiologyPomeranian Academy of EducationSlupskPoland

Personalised recommendations