Acta Physiologiae Plantarum

, Volume 24, Issue 4, pp 365–370 | Cite as

Growth and photosynthetic activity of micropropagated strawberry plants inoculated with endomycorrhizal fungi (AMF) and growing under drought stress

  • Bożenna Borkowska


The plants produced by in vitro methods are free of any microflora contrary to natural systems where plants are colonized by symbiotic fungi. The present paper reports the experiments carried out to evaluate the role of arbuscular endomycorrhizal fungi in development of micropropagated strawberries and their photosynthetic activity (measured by chlorophyll fluorescence) under drought conditions.

Mycorrhization strongly affected growth and tolerance to water deficiency of the plants cultivated in greenhouse. Wilting of not-mycorrhized plants was accompanied by drastic increase of Fo and Tfm and decrease of Fm. At the same time, the value of these parameters for mycorrhized plants did not change. Drastic decrease in the value of parameters Fv/Fm, Fv/Fo and Fo/Fm for plants without AMF appeared at the end of dry period. Rise of Fs and decrease Rfd was noted only for not-mycorrhized plants. The plants colonized by fungi, fully recovered their photosynthetic activity when watering was restored.

Key words

chlorophyll fluorescence growth micropropagation mycorrhization (AMF) strawberry drought stress 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Auge R.M. 2001. Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza 11: 3–42.CrossRefGoogle Scholar
  2. Borkowska B., Szczygiel A., Pierzga K. 1999. Direct ex vitro rooting technique of micropropagated strawberry shoots. J. Fruit Ornam. Plant Res. 7: 1–10.Google Scholar
  3. Boxus Ph. 1974. The production of strawberry plants by in vitro micropropagation. J. Hort. Sci. 49: 209–210.Google Scholar
  4. Dood J.C. 2000. The role of arbuscular mycorrhizal fungi in agro- and natural ecosystems. Outlook on Agriculture 29: 55–62.CrossRefGoogle Scholar
  5. El-Tohamy W., Schnitzler W.H., El-Behairy U., El-Beltagy M.S. 1999. Effect of VA mycorrhiza on improving drought and chilling tolerance of bean plants (Phasoleus vulgaris L.) J. Appl. Bot. - Angew. Bot. 73: 178–183.Google Scholar
  6. Estrada-Luna A.A., Davies F.T., Egilla J.N. 2000. Mycorrhizal fungi enhancement of growth and gas exchange of micropropagated guava plantlets (Psidium guajava L.) during ex vitro acclimatization and plant establishment. Mycorrhiza 10: 1–8.CrossRefGoogle Scholar
  7. Guillemin J.P., Gianinazzi S., Trouvelot A. 1992. Screening of arbuscular endomycorrhizal fungi for establishment of micropropagated pineapple plants. Agronomie 12: 831–836.Google Scholar
  8. Hooker J.H., Gianinazzi S., Vestberg M., Barea J.M., Atkinson D. 1994. The application of arbuscular fungi to micropropagation systems: an opportunity to reduce chemical inputs. Agric. Sci. in Finland 3: 227–232.Google Scholar
  9. Krause G.H., Weis E. 1984. Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals. PHotosynth. Res. 5: 139–157.CrossRefGoogle Scholar
  10. Lichtenthaler H.K., Buschman C., Rinderle U., Schmuck G. 1986. Application of chlorophyll fluorescence in ecophysiology. Radiat. Environ. Biophys. 25: 297–308.PubMedCrossRefGoogle Scholar
  11. Schreiber U., Bilger W., Neubauer C. 1994. Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. In: Ecophysiology of PHotosynthesis, ed. by Schulze E.D., Caldwell M.M.: 49–70.Google Scholar
  12. Strasser R.J., Srivastava A., Tsimilli-Michael M. 2001. Screening the vitality and photosynthetic activity of plants by the fluorescence transient. Workshop: Phenomenology of the Fluorescence Transient. Warsaw.Google Scholar

Copyright information

© Department of Plant Physiology 2002

Authors and Affiliations

  • Bożenna Borkowska
    • 1
  1. 1.Research Institute of Pomology and FloricultureSkierniewicePoland

Personalised recommendations