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Acta Physiologiae Plantarum

, Volume 24, Issue 1, pp 3–10 | Cite as

Changes in the composition of fatty acids and sterols of membrane lipids during induction and differentiation of Brassica napus (var. oleifera L.) callus

  • Iwona Żur
  • Andrzej Skoczowski
  • Elżbieta Niemczyk
  • Franciszek Dubert
Article

Abstract

Changes in the membrane lipid and sterols content and composition were studied during induction and differentiation in callus cultures of Brassica napus var. oleifera. Callus induction was associated with an increase of DGDG content, significant changes in fatty acids composition of all lipid fractions and increased degree of lipid unsaturation. The membrane lipid composition of tissue at different degrees of differentiation was found to vary significantly, particularly two weeks after transfer of callus to regeneration medium. The main differences concerned the content and composition of galactolipids. Curiously in many cases, these differences declined during subsequent culture, in spite of the morphogenesis process which was in progress. Another result of differentiation was the change in free sterol composition: in shoot regenerating calli the content of stigmasterol had rose whereas the accumulation of campesterol decreased. Even though observed changes in membrane properties may not play a role in morphogenesis they are nevertheless useful as developmental markers and can be invaluable in understanding biochemical basis of morphogenesis.

Key words

Brassica napus callus fatty acid composition galactolipids phospholipids oilseed rape regeneration ability sterols 

List of abbreviations

16:0

palmitic acid

16:1

palmitooleic acid

17:0

margaric acid

18:0

stearic acid

18:1

oleic acid

18:2

linoleic acid

18:3

linolenic acid

18:3/18:2

ratio of 18:3/18:2 fatty acids

BAP

6-benzylaminopurine

BF3

boron trifluoride

2,4-D

2,4-dichlorophenoxyacetic acid

DBI

Double Bound Index = Σ (molar % fatty acid content·number of double bounds)/100

GA3

gibberellic acid

MGDG

monogalactosyl diacylglycerol

DGDG

digalactosyl diacylglycerol

MS

basal Murashige and Skoog medium

PL

phospholipids

PL/GL

ratio of phospholipids to glycolipids

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Copyright information

© Department of Plant Physiology 2002

Authors and Affiliations

  • Iwona Żur
    • 1
  • Andrzej Skoczowski
    • 1
  • Elżbieta Niemczyk
    • 1
  • Franciszek Dubert
    • 1
  1. 1.Department of Plant Physiology Polish Academy of SciencesThe Franciszek GórskiKrakówPoland

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