Acta Physiologiae Plantarum

, Volume 23, Issue 3, pp 273–277

Antioxidant enzymes and membrane lipid composition of disease resistant tomato plants regenerated from crown galls

  • B. Barna
  • M. Pogány
Article

Abstract

Inhibition of senescence of plants increases their resistance to necrotrophic pathogens and to many abiotic stresses. Tomato plants regenerated from tumors induced by Agrobacterium tumefaciens T37 strain showed higher degree of tolerance to Phytophthora infestans infection. As regards the mechanism of the enhanced resistance to necrosis, increased phospholipid and galactolipid contents, in addition to decreased free sterols in the transformed plants gave greater stability to their membranes as compared to membranes from control plants. In addition, the elevated activity of some antioxidant enzymes such as superoxide dismutase and catalase in transformed tissues can also play a significant role in the tolerance to pathogenic and abiotic stresses

Key words

tomato juvenility superoxide dismutase catalase peroxidase phospholipids galactolipids free sterols Phytophthora infestans resistance 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ádám, A.L., Bestwick, C.S., Barna, B. Mansfield, J.W. 1995. Enzymes regulating the accumulation of active oxygen species during the hypersensitive reaction to Pseudomonas syringae pv. phaseolicola. Planta, 196: 240–249.Google Scholar
  2. Baker, C.J., Orlandi, E.W. 1995. Active oxygen in plant pathogenesis. Annu. Rev. Phytopathol., 33: 299–321.CrossRefGoogle Scholar
  3. Barna B., Ibenthal W.D., Heitefuss R. 1989 Extracellular RNase activity in healthy and rust infected wheat leaves. Physiol. Molec. Plant Pathol., 35: 151–160.CrossRefGoogle Scholar
  4. Barna, B., Györgyi, B. 1992. Resistance of young versus old tobacco leaves to necrotrophs, fusaric acid, cell-wall degrading enzymes and autolysis of membrane lipids. Physiol. Molec. Plant Pathol., 40: 247–257.CrossRefGoogle Scholar
  5. Barna, B., Ádám, A., Király, Z. 1993. Juvenility and resistance of a superoxide-tolerant plant to diseases and other stresses. Naturwissenschaften, 80: 420–422.CrossRefGoogle Scholar
  6. Barna, B., Ádám, A.L., Gullner, G., Király, Z. 1995. The role of antioxidant systems and juvenility in tolerance of plants to diseases and abiotic stresses. Acta Phytopath. Entom. Hung., 30: 39–45.Google Scholar
  7. Barna, B., Ádám, A.L., Király, Z. 1997. Increased levels of cytokinin induce tolerance to necrotic diseases and various oxidative stress-causing agents in plants. Phyton 37,: 25–30.Google Scholar
  8. Elad, Y. 1993. Regulators of ethylene biosynthesis or activity as a tool for reducing susceptibility of host plant tissues to infection by Botrytis cinerea.-Neth. J. Pl. Path., 99: 105–113.Google Scholar
  9. Elstner, E.F., Osswald, W. 1994. Mechanism of oxygen activation during plant stress. — In Oxigen and Environmental Stress in Plants (R.M.M. Crawford, G.A.F. Hendry and B.A. Goodman, eds), 131–154 The Royal Society of Edinburgh, 102B, Edinburgh.Google Scholar
  10. Fodor, J., Gullner, G., Ádám, A.L., Barna, B., Kőmíves, T., Király, Z. 1997. Local and systemic responses of antioxidants to tobacco mosaic virus infection and to salicylic acid in tobacco: Role in systemic acquired resistance. Plant Physiol., 114: 1443–1451.PubMedGoogle Scholar
  11. Galal, A.A., Barna, B., Érsek, T. 1991. Use of toxic substances from Phytophthora infestans (Mont.) de Bary in screening for late blight resistant potatoes. Acta Phytopath. et Entom. Hung., 26: 263–270.Google Scholar
  12. Goodman, R.N., Novacky, A. 1994. The Hypersensitive Defence Reactions in Plants. St. Paul, Mn, APS Press. pp. 244Google Scholar
  13. Grant, M., Mansfield, J. 1999. Early events in host-pathogen interactions. Current Opinion in Plant Biology, 2: 312–319.PubMedCrossRefGoogle Scholar
  14. Karpinski, S., Reynolds, H., Karpinska, B., Wingsle, G., Creissen, G. Mullineaux, P. 1999. Systemic sygnalling and acclimation response to excess excitation energy in Arabidopsis. Science, 284: 654–657.PubMedCrossRefGoogle Scholar
  15. Király, Z., El-Zahaby, H., Galal, A., Abdou, S., Ádám, A., Barna, B., Klement, Z. 1993. Effect of oxy free radicals on plant pathogenic bacteria and fungi and on some plant diseases. — In: Oxygen Free Radicals and Scavengers in the Natural Sciences (Gy. Mózsik, I. Emerit, J. Fehér, B. Matkovics and Á. Vincze, eds), pp. 9–19. Akadémia Kiadó, Budapest.Google Scholar
  16. Leshem, Y. 1988. Plant senescence processes and free radicals. Free Radic. Biol. and Medic. 5: 39–49.CrossRefGoogle Scholar
  17. Low, P., Merida, J. 1996. The oxidative burst in plant defence: Function and signal transduction. Physiol. Plant., 96: 533–542.CrossRefGoogle Scholar
  18. Necasek, J., Dusbábková, J. & Pekarková-Troníckova, E. 1988. Whole plants regeneration from crown galls of Lycopersicon esculentum.-Biologia Plantarum, 30: 1–8.Google Scholar
  19. Ouf, M.F., Gazar, A.A., Shehata, Z.A., Abdou, El S., Király, Z., Barna, B. 1993. The effect of superoxide anion on germination and infectivity of wheat stem rust (Puccinia graminis Pers. f. sp. tritici Eriks. and Henn.) uredospores. Cereal Research Communications 21: 31–37.Google Scholar
  20. Peng, M., Kuc, J. 1992. Peroxidase-generated hydrogen peroxide as a source of antifungal activity in vitro and on tobacco leaf disks. Phytopathol., 82: 696–699.Google Scholar
  21. Racchi, M., Terragna, C. 1993. Catalase isozymes are useful markers of differentiation in maize tissue cultures. Plant Science, 93: 195–202.CrossRefGoogle Scholar
  22. Sutherland, M.W. 1991. The generation of oxygen free radical during host plant response to infection. Physiol. Molec. Plant Pathol., 39: 79–93.CrossRefGoogle Scholar
  23. Vigh, L., Horváth, I., Horváth, L.I., Dudits, D., Farkas, T. 1979. Protoplast plasmalemma fluidity of hardened wheats correlates with frost resistance. FEBS Lett. 107, 291–294.PubMedCrossRefGoogle Scholar

Copyright information

© Department of Plant Physiology 2001

Authors and Affiliations

  • B. Barna
    • 1
  • M. Pogány
    • 1
  1. 1.Plant Protection InstituteHungarian Academy of SciencesBudapestHungary

Personalised recommendations