Effects of electroacupuncture of different frequencies on SP and VIP expression levels in colon of rats with slow transit constipation
To observe the effects of electroacupuncture (EA) of different frequencies on the expression levels of substance P (SP) and vasoactive intestinal peptide (VIP) in the colon of rats with slow transit constipation (STC).
One hundred healthy male Sprague-Dawley (SD) rats were randomly divided into a normal group, a model group, a low-frequency EA group, a high-frequency EA group and a variable-frequency EA group, with 20 rats in each group. The rats in the normal group were fed with normal diet. The rats in the other groups were fed with phenethylpiperidine in the diet at a dose of 8 mg/(kg·bw) per day, for 120 d, to establish the STC model. Rats in the normal group and the model group did not receive any treatment; rats in the low-frequency EA group were treated with 2 Hz continuous wave EA, rats in the high-frequency EA group were treated with 100 Hz continuous wave EA, and rats in the variable-frequency EA group were treated with 2 Hz/100 Hz sparse-dense EA. The current intensity of the EA was determined by the slight vibration of the rat limbs without painful screaming. The intervention was performed once a day, 15 min/time for continuous 15 d. After treatment, the intestinal transit function and the expression levels of SP and VIP in the colon of the rats in each group were determined.
After treatment, the defecation duration of the first dark stool in the model group was significantly longer than that in the normal group (P<0.05); the defecation durations of the first dark stool in the low-frequency EA group, high-frequency EA group and variable-frequency EA group were significantly shorter than the duration in the model group (all P<0.05); compared with the low-frequency EA group, the first dark stool defecation duration of rats in the variable-frequency EA group was significantly shorter (P<0.05); compared with the normal group, the SP and VIP expression levels in the colon of the model group were significantly decreased (both P<0.01); the SP and VIP expression levels in the colon of the low-frequency EA group, the high-frequency EA group and the variable-frequency EA group were significantly higher than those in the model group (all P<0.05); compared with the high-frequency EA group, the SP expression levels in the colon in the low-frequency EA group and the variable-frequency EA group were significantly increased (both P<0.05); compared with the low-frequency EA group, the VIP expression levels in the colon in the high-frequency EA group and the variable-frequency EA group were significantly increased (both P<0.05).
EA improves the intestinal function of STC model rats by regulating the expression levels of SP and VIP in rat colon. The EA stimulation with 100 Hz continuous wave, 2 Hz/100 Hz sparse-dense wave shows a better improvement in the colonic transit function in STC rats, followed by 2 Hz continuous wave.
KeywordsAcupuncture Therapy Electroacupuncture Constipation Substance P Vasoactive Intestinal Peptide Colon Rats
将健康雄性Sprague-Dawley (SD)大鼠100只随机分为正常组、模型组、低频电针组、高频电针组和变频电针组, 每组20只。正常组大鼠以普通饲料喂养, 其余组大鼠在饲料中添加复方苯乙哌啶, 每日8 mg/(kg·bw), 连续 给药120 d, 建立STC模型。正常组和模型组不进行任何治疗; 低频电针组给予2 Hz连续波电针治疗, 高频电针组给予100 Hz连续波电针治疗, 变频电针组给予2 Hz/100 Hz疏密波电针治疗。电针电流强度以大鼠肢体微微颤动而不 痛苦尖叫为度。每日1次, 每次治疗15 min, 共治疗15 d。治疗后, 测定各组大鼠肠道传输功能和结肠中SP和VIP的 含量水平。
治疗后, 与正常组比较, 模型组大鼠首粒黑便排出时间明显延长(P<0.05); 低频电针组、高电针组和变频电针组大鼠首粒黑便排出时间均较模型组明显缩短(均P<0.05); 变频电针组大鼠首粒黑便排出时间较低频电针组明显缩短(P<0.05)。与正常组比较, 模型组大鼠结肠SP和VIP含量明显降低(均P<0.01); 低频电针 组、高频电针组和变频电针组大鼠的结肠SP和VIP含量均较模型组明显升高(均P<0.05)。与高频电针组比较, 低频电针组和变频电针组大鼠的结肠SP含量明显升高(均P<0.05); 与低频电针组比较, 高频电针组和变频电针组大鼠 的结肠VIP含量明显升高(均P<0.05)。
电针疗法可通过调节大鼠结肠中SP和VIP的含量改善STC模型大鼠的肠道功能; 100 Hz连续波和2 Hz/100 Hz疏密波电针刺激对改善STC大鼠的结肠传输功能相对较好, 2 Hz连续波的作 用次之。
关键词针刺疗法 电针 便秘 P 物质 血管活性肠肽 结肠 大鼠
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This work was supported by National Natural Science Foundation of China (国家自然科学基金项目, No. 81473773); Project of Hebei Provincial Natural Science Foundation of China (河北省自然科学基金项目, No. H2015423048).
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