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Frontiers of Materials Science

, Volume 6, Issue 1, pp 47–59 | Cite as

The role of crystallinity on differential attachment/proliferation of osteoblasts and fibroblasts on poly (caprolactone-co-glycolide) polymeric surfaces

  • Helen CuiEmail author
  • Patrick J. Sinko
Research Article

Abstract

The objective of the present study is to systematically evaluate the role of polymer crystallinity on fibroblast and osteoblast adhesion and proliferation using a series of poly(caprolactone-co-glycolide) (PCL/PGA) polymers. PCL/PGA polymers were selected since they reflect both highly crystalline and amorphous materials. PCL/PGA polymeric materials were fabricated by compression molding into thin films. Five compositions, from PCL or PGA to intermediate copolymeric compositions of PCL/PGA in ratios of 25:75, 35:65 and 45:55, were studied. Pure PCL and PGA represented the crystalline materials while the copolymers were amorphous. The polymers/copolymers were characterized using DSC to assess crystallinity, contact angle measurement for hydrophobicity, and AFM for nanotopography. The PCL/PGA films demonstrated similar hydrophobicity and nanotopography whereas they differed significantly in crystallinity. Cell adhesion to and proliferation on PCL/PGA films and proliferation studies were performed using osteoblasts and NIH-3T3 fibroblasts. It was observed that highly crystalline and rigid PCL and PGA surfaces were significantly more efficient in supporting fibroblast growth, whereas amorphous/flexible PCL/PGA 35:65 was significantly more efficient in supporting growth of osteoblasts. This study demonstrated that while chemical composition, hydrophobicity and surface roughness of PCL/PGA polymers were held constant, crystallinity and rigidity of PCL/PGA played major roles in determining cell responses.

Keywords

crystallinity attachment proliferation osteoblast fibroblast PCL-PGA 

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References

  1. [1]
    Anderson J M, Rodriguez A, Chang D T. Foreign body reaction to biomaterials. Seminars in Immunology, 2008, 20(2): 86–100CrossRefGoogle Scholar
  2. [2]
    Yang X B, Roach H I, Clarke N M, et al. Human osteoprogenitor growth and differentiation on synthetic biodegradable structures after surface modification. Bone, 2001, 29(6): 523–531CrossRefGoogle Scholar
  3. [3]
    Harber G M. Cell-material interactions: fundamental design issues for tissue engineering and clinical considerations. In: Guelcher S A, Hollinger J O, eds. An Introduction to Biomaterials. Boca Raton, FL, USA: CRC Press/Taylor & Francis Group, 2006, 189–210Google Scholar
  4. [4]
    Kalbacova M, Rezek B, Baresova V, et al. Nanoscale topography of nanocrystalline diamonds promotes differentiation of osteoblasts. Acta Biomaterialia, 2009, 5(8): 3076–3085CrossRefGoogle Scholar
  5. [5]
    Biggs D L, Lengsfeld C S, Hybertson B M, et al. In vitro and in vivo valuation of the effects of PLA microparticle crystallinity on cellular response. Journal of Controlled Release, 2003, 92(1–2): 147–161CrossRefGoogle Scholar
  6. [6]
    Degirmenbasi N, Ozkan S, Kalyon D M, et al. Surface patterning of poly(L-lactide) upon melt processing: In vitro culturing of fibroblasts and osteoblasts on surfaces ranging from highly crystalline with spherulitic protrusions to amorphous with nanoscale indentations. Journal of Biomedical Materials Research Part A, 2009, 88A(1): 94–104CrossRefGoogle Scholar
  7. [7]
    Kawamoto N, Mori H, Terano M, et al. Blood compatibility of polypropylene surfaces in relation to the crystalline-amorphous microstructure. Journal of Biomaterials Science, Polymer Edition, 1997, 8(11): 859–877CrossRefGoogle Scholar
  8. [8]
    Park A, Cima L G. In vitro cell response to differences in poly-Llactide crystallinity. Journal of Biomedical Materials Research, 1996, 31(1): 117–130CrossRefGoogle Scholar
  9. [9]
    Wang S, Kempen D H, Yaszemski M J, et al. The roles of matrix polymer crystallinity and hydroxyapatite nanoparticles in modulating material properties of photo-crosslinked composites and bone marrow stromal cell responses. Biomaterials, 2009, 30(20): 3359–3370CrossRefGoogle Scholar
  10. [10]
    Washburn N R, Yamada K M, Simon C G Jr, et al. Highthroughput investigation of osteoblast response to polymer crystallinity: influence of nanometer-scale roughness on proliferation. Biomaterials, 2004, 25(7–8): 1215–1224CrossRefGoogle Scholar
  11. [11]
    Winet H, Bao J Y. Comparative bone healing near eroding polylactide-polyglycolide implants of differing crystallinity in rabbit tibial bone chambers. Journal of Biomaterials Science, Polymer Edition, 1997, 8(7): 517–532CrossRefGoogle Scholar
  12. [12]
    Wang D, Christensen K, Chawla K, et al. Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. Journal of Bone and Mineral Research, 1999, 14(6): 893–903CrossRefGoogle Scholar
  13. [13]
    Agrawal C M, Ray R B. Biodegradable polymeric scaffolds for musculoskeletal tissue engineering. Journal of Biomedical Materials Research, 2001, 55(2): 141–150CrossRefGoogle Scholar
  14. [14]
    Tiaw K S, Teoh S H, Chen R, et al. Processing methods of ultrathin poly(ɛ-caprolactone) films for tissue engineering applications. Biomacromolecules, 2007, 8(3): 807–816CrossRefGoogle Scholar
  15. [15]
    Cheng Z, Teoh S-H. Surface modification of ultra thin poly (ɛ-caprolactone) films using acrylic acid and collagen. Biomaterials, 2004, 25(11): 1991–2001CrossRefGoogle Scholar
  16. [16]
    Bramfeldt H, Vermette P. Enhanced smooth muscle cell adhesion and proliferation on protein-modified polycaprolactone-based copolymers. Journal of Biomedical Materials Research Part A, 2009, 88A(2): 520–530CrossRefGoogle Scholar
  17. [17]
    Chung T-W, Wang Y-Z, Huang Y-Y, et al. Poly (ɛ-caprolactone) grafted with nano-structured chitosan enhances growth of human dermal fibroblasts. Artificial Organs, 2006, 30(1): 35–41CrossRefGoogle Scholar
  18. [18]
    Ishaug-Riley S L, Okun L E, Prado G, et al. Human articular chondrocyte adhesion and proliferation on synthetic biodegradable polymer films. Biomaterials, 1999, 20(23–24): 2245–2256CrossRefGoogle Scholar
  19. [19]
    Lee S-H, Kim B-S, Kim S H, et al. Elastic biodegradable poly (glycolide-co-caprolactone) scaffold for tissue engineering. Journal of Biomedical Materials Research Part A, 2003, 66A(1): 29–37CrossRefGoogle Scholar
  20. [20]
    Otten J E, Wiedmann-Al-Ahmad M, Jahnke H, et al. Bacterial colonization on different suture materials — a potential risk for intraoral dentoalveolar surgery. Journal of Biomedical Materials Research Part B, Applied Biomaterials, 2005, 74B(1): 627–635CrossRefGoogle Scholar
  21. [21]
    Kowalczyńska H M, Kołos R, Nowak-Wyrzykowska M, et al. Atomic force microscopy evidence for conformational changes of fibronectin adsorbed on unmodified and sulfonated polystyrene surfaces. Journal of Biomedical Materials Research Part A, 2009, 91A(4): 1239–1251CrossRefGoogle Scholar
  22. [22]
    Ajami-Henriquez D, Rodríguez M, Sabino M, et al. Evaluation of cell affinity on poly(L-lactide) and poly(ɛ-caprolactone) blends and on PLLA-b-PCL diblock copolymer surfaces. Journal of Biomedical Materials Research Part A, 2008, 87A(2): 405–417CrossRefGoogle Scholar
  23. [23]
    Pelham R J Jr, Wang Y. Cell locomotion and focal adhesions are regulated by substrate flexibility. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94 (25): 13661–13665CrossRefGoogle Scholar
  24. [24]
    Tzvetkova-Chevolleau T, Stéphanou A, Fuard D, et al. The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure. Biomaterials, 2008, 29(10): 1541–1551CrossRefGoogle Scholar
  25. [25]
    Mo X, Weber H-J, Ramakrishna S. PCL-PGLA composite tubular scaffold preparation and biocompatibility investigation. The International Journal of Artificial Organs, 2006, 29(8): 790–799Google Scholar
  26. [26]
    Pamula E, Dobrzynski P, Szot B, et al. Cytocompatibility of aliphatic polyesters — In vitro tudy on fibroblasts and macrophages. Journal of Biomedical Materials Research Part A, 2008, 87A(2): 524–535CrossRefGoogle Scholar
  27. [27]
    Tsai W B, Chen C H, Chen J F, et al. The effects of types of degradable polymers on porcine chondrocyte adhesion, proliferation and gene expression. Journal of Materials Science: Materials in Medicine, 2006, 17(4): 337–343CrossRefGoogle Scholar
  28. [28]
    Tang Z G, Callaghan J T, Hunt J A. The physical properties and response of osteoblasts to solution cast films of PLGA doped polycaprolactone. Biomaterials, 2005, 26(33): 6618–6624CrossRefGoogle Scholar
  29. [29]
    Müller A J, Albuerne J, Marquez L, et al. Self-nucleation and crystallization kinetics of double crystalline poly(p-dioxanone)-b-poly(ɛ-caprolactone) diblock copolymers. Faraday Discussions, 2005, 128: 231–252CrossRefGoogle Scholar
  30. [30]
    Hamley I W, Castelletto V, Castillo R V, et al. Crystallization in poly(L-lactide)-b-poly(ɛ-caprolactone) double crystalline diblock copolymers: A study using X-ray scattering, differential scanning calorimetry, and polarized optical microscopy. Macromolecules, 2005, 38(2): 463–472CrossRefGoogle Scholar
  31. [31]
    Gough J E, Christian P, Scotchford C A, et al. Craniofacial osteoblast responses to polycaprolactone produced using a novel boron polymerisation technique and potassium fluoride posttreatment. Biomaterials, 2003, 24(27): 4905–4912CrossRefGoogle Scholar

Copyright information

© Higher Education Press and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Advanced Technology and Regenerative Medicine (ATRM)LLCSomervilleUSA
  2. 2.Department of Pharmaceutics, Ernest Mario School of Pharmacy, RutgersThe State University of New JerseyPiscatawayUSA

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