Advertisement

Frontiers of Chemical Science and Engineering

, Volume 12, Issue 4, pp 798–805 | Cite as

Biomimetic mineralization and cytocompatibility of nanorod hydroxyapatite/graphene oxide composites

  • Peizhen Duan
  • Juan ShenEmail author
  • Guohong Zou
  • Xu Xia
  • Bo JinEmail author
Research Article
  • 16 Downloads

Abstract

Nanorod hydroxyapatite (NRHA)/graphene oxide (GO) composites with weight ratios of 0.4, 1.5, and 5 have been fabricated by a facile ultrasonic-assisted method at room temperature and atmospheric pressure. The chemical structure properties and morphology of the composites were characterized by field emission source scanning electron microscope, X-ray diffraction, transmission electron microscopy, and high-resolution transmission electron microscopy. The results indicate that the NRHA/ GO composites have an irregular surface with different degree wrinkles and are stable, and NRHA are well combined with GO. In addition, the biomimetic mineralization mechanism of hydroxyapatite on the NRHA/GO composites in simulated body fluid (SBF) is presented. The presence of a bone-like apatite layer on the composite surface indicate that the NRHA/GO composites facilitate the nucleation and growth of hydroxyapatite crystals in SBF for biomimetic mineralization. Moreover, the NRHA- 1.5/GO composite and pure GO were cultured with MC3T3-E1 cells to investigate the proliferation and adhesion of cells. In vitro cytocompatibility evaluation demonstrated that the NRHA/GO composite can act as a good template for the growth and adhesion of cells. Therefore, the NRHA/GO composite could be applied as a GO-based, free-template, non-toxic, and bioactive composite to substitute for a damaged or defect bone.

Keywords

hydroxyapatite graphene oxide biomimetic mineralization cytocompatibility 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Notes

Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Grant Nos. 21201142 and 11502158) and Southwest University of Science and Technology Researching Project (Grant No. 14tdsc03).

References

  1. 1.
    Shi Y Y, Li M, Liu Q, Jia Z J, Xu X C, Cheng Y, Zheng Y F. Electrophoretic deposition of graphene oxide reinforced chitosanhydroxyapatite nanocomposite coatings on Ti substrate. Journal of Materials Science. Materials in Medicine, 2016, 27(3): 48CrossRefGoogle Scholar
  2. 2.
    Li M, Liu Q, Jia Z J, Xu X C, Shi Y Y, Cheng Y, Zheng Y F, Xi T F, Wei S C. Electrophoretic deposition and electrochemical behavior of novel graphene oxide-hyaluronic acid-hydroxyapatite nanocomposite coatings. Applied Surface Science, 2013, 284: 804–810CrossRefGoogle Scholar
  3. 3.
    Li P, Sun S Y, Dong A, Hao Y P, Shi S Q, Sun Z J, Gao G, Chen Y X. Developing of a novel antibacterial agent by functionalization of graphene oxide with guanidine polymer with enhanced antibacterial activity. Applied Surface Science, 2015, 355: 446–452CrossRefGoogle Scholar
  4. 4.
    Wang K W, Zhu Y J, Chen F, Cheng G F, Huang Y H. Microwaveassisted synthesis of hydroxyapatite hollow microspheres in aqueous solution. Materials Letters, 2011, 65(15-16): 2361–2363CrossRefGoogle Scholar
  5. 5.
    Kumar S, Chatterjee K. Comprehensive review on the use of graphene-based substrates for regenerative medicine and biomedical devices. ACS Applied Materials & Interfaces, 2016, 8(40): 26431–26457CrossRefGoogle Scholar
  6. 6.
    Liu L P, Yang X N, Ye L, Xue D D, Liu M, Jia S R, Hou Y, Chu L Q, Zhong C. Preparation and characterization of a photocatalytic antibacterial material: Graphene oxide/TiO2/bacterial cellulose nanocomposite. Carbohydrate Polymers, 2017, 174: 1078–1086CrossRefGoogle Scholar
  7. 7.
    Li Q, Yong C Y, Cao W W, Wang X, Wang L N, Zhou J, Xing X D. Fabrication of charge reversible graphene oxide-based nanocomposite with multiple antibacterial modes and magnetic recyclability. Journal of Colloid and Interface Science, 2017, 511: 285–295CrossRefGoogle Scholar
  8. 8.
    Xie X Y, Hu K W, Fang D D, Shang L H, Tran S D, Cerruti M. Graphene and hydroxyapatite self-assemble into homogeneous, free standing nanocomposite hydrogels for bone tissue engineering. Nanoscale, 2015, 7(17): 7992–8002CrossRefGoogle Scholar
  9. 9.
    Moosavi R, Ramanathan S, Lee Y Y, Siew L K C, Afkhami A, Archunan G, Padmanabhan P, Gulyás B, Kakran M, Selvan S T. Synthesis of antibacterial and magneticnanocomposites by decorating graphene oxide surface with metal nanoparticles. RSC Advances, 2015, 5(93): 76442–76450CrossRefGoogle Scholar
  10. 10.
    Wei G, Zhang J T, Xie L, Jandt K D. Biomimetic growth of hydroxyapatite on super water-soluble carbon nanotube-protein hybrid nanofibers. Carbon, 2011, 49(7): 2216–2226CrossRefGoogle Scholar
  11. 11.
    Wei G, Reichert J, Bossert J, Jandt K D. Novel biopolymeric template for the nucleation and growth of hydroxyapatite crystals based on self-assembled fibrinogen fibrils. Biomacromolecules, 2008, 9(11): 3258–3267CrossRefGoogle Scholar
  12. 12.
    Wang J H, Wang H X, Wang Y Z, Li J F, Su Z Q, Wei G. Alternate layer-by-layer assembly of graphene oxide nanosheets and fibrinogen nanofibers on a silicon substrate for a biomimetic threedimensional hydroxyapatite scaffold. Journal of Materials Chemistry. B, Materials for Biology and Medicine, 2014, 2(42): 7360–7368CrossRefGoogle Scholar
  13. 13.
    Kaur B, Srivastava R, Satpati B, Kondepudi K K, Bishnoi M. Biomineralization of hydroxyapatite in silver ion-exchanged nanocrystalline ZSM-5 zeolite using simulated body fluid. Colloids and Surfaces. B, Biointerfaces, 2015, 135: 201–208CrossRefGoogle Scholar
  14. 14.
    Yu W, Wang X X, Zhao J L, Tang Q G, Wang M L, Ning X W. Preparation and mechanical properties of reinforced hydroxyapatite bone cement with nano-ZrO2. Ceramics International, 2015, 41(9): 10600–11060CrossRefGoogle Scholar
  15. 15.
    Shen J, Jin B, Qi Y C, Jiang Q Y, Gao X F. Carboxylated chitosan/silver-hydroxyapatite hybrid microspheres with improved antibacterial activity and cytocompatibility. Materials Science and Engineering C, 2017, 78: 589–597CrossRefGoogle Scholar
  16. 16.
    Jadalannagari S, Deshmukh K, Ramanan S R, Kowshik M. Antimicrobial activity of hemocompatible silver doped hydroxyapatite nanoparticles synthesized by modified sol-gel technique. Applied Nanoscience, 2013, 4(2): 133–141CrossRefGoogle Scholar
  17. 17.
    Lin K L, Wu C T, Chang J. Advances in synthesis of calcium phosphate crystals with controlled size and shape. Acta Biomaterialia, 2014, 10(10): 4071–4102CrossRefGoogle Scholar
  18. 18.
    Raucci M G, Giugliano D, Longo A, Zeppetelli S, Carotenuto G, Ambrosio L. Comparative facile methods for preparing graphene oxide-hydroxyapatite for bone tissue engineering. Journal of Tissue Engineering and Regenerative Medicine, 2016, 11(8): 2204–2216CrossRefGoogle Scholar
  19. 19.
    Santos C, Almeida M M, Costa M E. Morphological evolution of hydroxyapatite particles in the presence of different citrate: Calcium ratios. Crystal Growth & Design, 2015, 15(9): 4417–4426CrossRefGoogle Scholar
  20. 20.
    Zhao X Y, Zhu Y J, Chen F, Lu B Q, Wu J. Nanosheet-assembled hierarchical nanostructures of hydroxyapatite: Surfactant-free microwave-hydrothermal rapid synthesis, protein/DNA adsorption and pH-controlled release. CrystEngComm, 2013, 15(1): 206–212CrossRefGoogle Scholar
  21. 21.
    Fan Z J, Wang J Q, Wang Z F, Ran H Q, Li Y, Niu L Y, Gong P W, Liu B, Yang S R. One-pot synthesis of graphene/hydroxyapatite nanorod composite for tissue engineering. Carbon, 2014, 66: 407–416CrossRefGoogle Scholar
  22. 22.
    Fielding G A, Roy M, Bandyopadhyay A, Bose S. Antibacterial and biological characteristics of silver containing and strontium doped plasma sprayed hydroxyapatite coatings. Acta Biomaterialia, 2012, 8(8): 3144–3152CrossRefGoogle Scholar
  23. 23.
    Gao F, Xu C Y, Hu H T, Wang Q, Gao Y Y, Chen H, Guo Q N, Chen D N, Eder D. Biomimetic synthesis and characterization of hydroxyapatite/graphene oxide hybrid coating on Mg alloy with enhanced corrosion resistance. Materials Letters, 2015, 138: 25–28CrossRefGoogle Scholar
  24. 24.
    Liu H Y, Xi P X, Xie G Q, Shi Y J, Hou F P, Huang L, Chen F J, Zeng Z Z, Shao C W, Wang J. Simultaneous reduction and surface functionalization of graphene oxide for hydroxyapatite mineralization. Journal of Physical Chemistry C, 2012, 116(5): 3334–3341CrossRefGoogle Scholar
  25. 25.
    Baradaran S, Moghaddam E, Basirun W J, Mehrali M, Sookhakian M, Hamdi M, Moghaddam N M R, Alias Y. Mechanical properties and biomedical applications of a nanotube hydroxyapatite-reduced graphene oxide composite. Carbon, 2014, 69: 32–45CrossRefGoogle Scholar
  26. 26.
    Bharath G, Madhu R, Chen S M, Veeramani V, Balamurugan A, Mangalaraj D, Viswanathan C, Ponpandian N. Enzymatic electrochemical glucose biosensors by mesoporous 1D hydroxyapatite-on-2D reduced graphene oxide. Journal of Materials Chemistry. B, Materials for Biology and Medicine, 2015, 3(7): 1360–1370CrossRefGoogle Scholar
  27. 27.
    Li M, Liu Q, Jia Z J, Xu X C, Cheng Y, Zheng Y F, Xi T F, Wei S C. Graphene oxide/hydroxyapatite composite coatings fabricated by electrophoretic nanotechnology for biological applications. Carbon, 2014, 67: 185–197CrossRefGoogle Scholar
  28. 28.
    Ma H B, Su W X, Tai Z X, Sun D F, Yan X B, Liu B, Xue Q J. Preparation and cytocompatibility of polylactic acid/hydroxyapatite/ graphene oxide nanocomposite fibrous membrane. Chinese Science Bulletin, 2012, 57(23): 3051–3058CrossRefGoogle Scholar
  29. 29.
    Zhu J T, Wong H M, Kwok Y K W, Tjong S C. Spark plasma sintered hydroxyapatite/graphite nanosheet and hydroxyapatite/ multiwalled carbon nanotube composites: Mechanical and in vitro cellular properties. Advanced Engineering Materials, 2011, 13(4): 336–341CrossRefGoogle Scholar
  30. 30.
    Rajesh R, Ravichandran D Y. Development of new graphene oxide incorporated tricomponent scaffolds with polysaccharides and hydroxyapatite and study of their osteoconductivity on MG-63 cell line for bone tissue engineering. RSC Advances, 2015, 5(51): 41135–41143CrossRefGoogle Scholar
  31. 31.
    Kokubo T, Takadama H. How useful is SBF in predicting in vivo bone bioactivity? Biomaterials, 2006, 27(15): 2907–2915CrossRefGoogle Scholar
  32. 32.
    Zhang M F, Li Y Z, Su Z Q, Wei G. Recent advances in the synthesis and applications of graphene-polymer nanocomposites. Polymer Chemistry, 2015, 6(34): 6107–6124CrossRefGoogle Scholar
  33. 33.
    Chen F, Zhu Y J, Wang K W, Zhao K L. Surfactant-free solvothermal synthesis of hydroxyapatite nanowire/nanotube ordered arrays with biomimetic structures. CrystEngComm, 2011, 13(6): 1858–1863CrossRefGoogle Scholar
  34. 34.
    Xiong G Y, Luo H L, Zuo G F, Ren K J, Wan Y Z. Novel porous graphene oxide and hydroxyapatite nanosheets-reinforced sodium alginate hybrid nanocomposites formedical applications. Materials Characterization, 2015, 107: 419–425CrossRefGoogle Scholar
  35. 35.
    Shen J, Jin B, Jiang Q Y, Hu Y M, Wang X Y. Morphologycontrolled synthesis of fluorapatite nano/microstructures via surfactant-assisted hydrothermal process. Materials & Design, 2016, 97: 204–212CrossRefGoogle Scholar
  36. 36.
    Roach P, Eglin D, Rohde K, Perry C C. Modern biomaterials: A review-bulk properties and implications of surface modifications. Journal of Materials Science. Materials in Medicine, 2007, 18(7): 1263–1277CrossRefGoogle Scholar
  37. 37.
    Mehrali M, Moghaddam E, Seyed S S F, Baradaran S, Mehrali M, Latibari S T, Cornelis M H S, Kadri N A, Zandi K, Abu O N A. Mechanical and in vitro biological performance of graphene nanoplatelets reinforced calcium silicate composite. PLoS One, 2014, 9(9): e106802CrossRefGoogle Scholar
  38. 38.
    Yu X Q, Wang Z P, Su Z Q, Wei G. Design, fabrication, and biomedical applications of bioinspired peptide-inorganic nanomaterial hybrids. Journal of Materials Chemistry. B, Materials for Biology and Medicine, 2017, 5(6): 1130–1142CrossRefGoogle Scholar
  39. 39.
    Li C X, Born A K, Schweizer T, Zenobi-Wong M, Cerruti M, Mezzenga R. Amyloid-hydroxyapatite bone biomimetic composites. Advanced Materials, 2014, 26(20): 3207–3212CrossRefGoogle Scholar
  40. 40.
    Liu Y, Huang J, Li H. Synthesis of hydroxyapatite-reduced graphite oxide nanocomposites for biomedical applications: Oriented nucleation and epitaxial growth of hydroxyapatite. Journal of Materials Chemistry. B, Materials for Biology and Medicine, 2013, 1(13): 1826CrossRefGoogle Scholar
  41. 41.
    Zhang Q, Liu Y, Zhang Y, Li H X, Tan Y N, Luo L L, Duan J H, Li K Y, Banks C E. Facile and controllable synthesis of hydroxyapatite/ graphene hybrid materials with enhanced sensing performance towards ammonia. Analyst (London), 2013, 00: 1–8Google Scholar
  42. 42.
    Ren J, Zhang X G, Chen Y. Graphene accelerates osteoblast attachment and biomineralization. Carbon Letters, 2017, 22: 42–44Google Scholar
  43. 43.
    Zhao X N, Zhang P P, Chen Y T, Su Z Q, Wei G. Recent advances in the fabrication and structure-specific applications of graphenebased inorganic hybrid membranes. Nanoscale, 2015, 7(12): 5080–5093CrossRefGoogle Scholar
  44. 44.
    Wen T, Wu X L, Liu M C, Xing Z H, Wang X K, Xu A W. Efficient capture of strontium from aqueous solutions using graphene oxidehydroxyapatite nanocomposites. Dalton Transactions (Cambridge, England), 2014, 43(20): 7464–7472CrossRefGoogle Scholar
  45. 45.
    Li D P, Liu T J, Yu X Q, Wu D, Su Z Q. Fabrication of graphenebiomacromolecule hybrid materials for tissue engineering application. Polymer Chemistry, 2017, 8(30): 4309–4321CrossRefGoogle Scholar
  46. 46.
    Zhang L, Liu W W, Yue C G, Zhang T H, Li P, Xing Z W, Chen Y. A tough graphene nanosheet/hydroxyapatite composite with improved in vitro biocompatibility. Carbon, 2013, 61: 105–115CrossRefGoogle Scholar

Copyright information

© Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Materials Science and EngineeringSouthwest University of Science and TechnologyMianyangChina
  2. 2.State Key Laboratory Cultivation Base for Nonmetal Composites and Functional MaterialsSouthwest University of Science and TechnologyMianyangChina

Personalised recommendations