Frontiers of Agriculture in China

, Volume 1, Issue 4, pp 449–455

Research progress on isolation and cloning of functional genes in tea plants



Tea, which has many sanitarian functions, is one of the most popular non-alcoholic soft and healthy beverages in the world. In many countries, as well as in China, tea (Camellia sinensis) is an important cash crop. It has great value as a source of secondary metabolic products. Molecular biology of tea plants has been one of the most active and kinetic research fields of tea science for the last decade. Isolation and cloning of important functional genes of tea plants have a critical significance on elucidating the molecular mechanism of high quality, yield and resistance, as well as genetic manipulating via biotechnological approaches for tea plants. In this paper, we introduced the research progress on the isolation and cloning of functional genes in tea plants. In addition, the brief prospect on the research of functional genes of tea plants in the near future is also give out.


tea plant (Camellia sinensisfunctional gene isolation and cloning 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Chen L, Zhao L P, Gao Q K (2005). Generation and analysis of expressed sequence tags from the tender shoot cDNA library of tea plant (Camellia sinensis). Plant Science, 168: 359–363CrossRefGoogle Scholar
  2. Edwards J W, Walker E L, Coruzzi G M (1990). Cell-specific expression in transgenic plants reveals nonoverlapping roles for chloroplast and cytosolic glutamine synthetase. Proceedings of the National Academy of Sciences of the United States of America, 87: 3459–3463PubMedCrossRefGoogle Scholar
  3. Feng Y F, Liang Y R (2001). Cloning and sequencing of S-adenosylmethionine synthetase gene in tea plant. Journal of Tea Science, 21(1): 21–25 (in Chinese)Google Scholar
  4. Kato M, Mizuno K, Crozier A, Fujimura T, Ashihara A (2000). Caffeine synthase gene from tea leaves. Nature, 406: 956–957PubMedCrossRefGoogle Scholar
  5. Kato M, Mizuno K, Fujimura T, Iwama M, Irie M, Corzier A, Ashihara H (1999). Purification and characterization of caffeine synthase from tea leaves. Plant Physiology, 120: 579–586PubMedCrossRefGoogle Scholar
  6. Li Y H, Jiang C J, Wan X C (2004a). Study on the expression of caffeine synthase gene mRNA in tea plant. Journal of Tea Science, 24(1): 23–28 (in Chinese)Google Scholar
  7. Li Y H, Jiang C J, Yang S L, Yu Y B (2004b). β-glucosidase cDNA cloning in the tea(Camellia sinensis) and its prokaryotic expression. Journal of Agricultural Biotechnology, 12(6): 625–629 (in Chinese)Google Scholar
  8. Matsumoto S, Takeuchi A, Hayatsu M (1994). Molecular cloning of phenylalanine ammonia-lyase cDNA and classification of varieties and cultivars of tea plants (Camellia sinensis) using the tea PAL cDNA probe. Theoretical and Applied Genetics, 89: 671–675CrossRefGoogle Scholar
  9. Mizutani M, Nakanishi H, Ema J, Ma S J, Noguchi E, Inohara-Ochiai M, Fukuchi-Mizutani M, Nakao M, Sakata K (2002). Cloning of β-primeverosidase from tea leaves, a key enzyme in tea aroma formation. Plant Physiology, 130: 2164–2176PubMedCrossRefGoogle Scholar
  10. Mondal T K, Bhattacharya A, Ahuja P S, Chand P (2001). Transgenic tea [Camellia sinensis (L.) O. Kuntze cv. Kangral Jat] plants obtained by Agrobacterium-mediated transformation of somatic embryos. Plant Cell Reporters, 20(8): 712–720CrossRefGoogle Scholar
  11. Park J S, Kim J B, Hahn B S, Kim K H, Ha S H, Kim J B, Kim Y H (2004). EST analysis of genes involved in secondary metabolism in Camellia sinensis (tea), using suppression subtractive hybridization. Plant Science, 166: 953–961CrossRefGoogle Scholar
  12. Schuler G D (1997). Pieces of the puzzle: Expressed sequence tags and the catalog of human genes. Journal of Molecular Medicine, 75: 694–698PubMedCrossRefGoogle Scholar
  13. Sugiyama T, Sadzuka Y (1999). Combination of theanine with doxorubicin inhibits hepatic metastasis of M5076 ovarian sarcoma. Clinical Cancer Research, 5(2): 413–416PubMedGoogle Scholar
  14. Takeo T (1981). Production of in linalool and geraniol by hydrolytic breakdown of bound forms in disrupted tea shoots. Phytochemistry, 120(9): 2145–2147CrossRefGoogle Scholar
  15. Takeuchi A, Matsumoto S, Hayatsu M (1994a). Chalcone synthase from Camellia sinensis isolation of the cDNAs and the organ-specific and sugar-responsive expression of the genes. Plant Cell Physiology, 35(7): 1011–1018PubMedGoogle Scholar
  16. Takeuchi A, Matsumoto S, Hayatsu M (1994b). Amplification of β-tubulin cDNA from Camellia sinensis by PCR. Bull Natl Res Inst Veg Ornam Plants & Tea, Japan (B), 7: 13–20Google Scholar
  17. Takeuchi A, Matsumoto S, Hayatsu M (1995). Effects of shading treatment on the expression of the genes for chalcone synthase and phenylalanine ammonia-lyase in tea plant (Camellia sinensis). Bull Natl Res Inst Veg Ornam Plants & Tea, Japan (B), 8: 1–9Google Scholar
  18. Wang C X, Li Y Y, Jiang C J, Yu Y B (2005). Molecular cloning and sequence analysis on cDNA of cystatin gene from tea leaves. Journal of Tea Science, 25(3): 177–182 (in Chinese)Google Scholar
  19. Wei C L, Jiang C J, Tao H Z, Wan X C (2003). Cloning and bioinformatics analysis of sequence signature of violaxanthin de-epoxidase cDNA in tea plant (Camellia sinensis (L.) O. Kuntze). Journal of Nanjing Agricultural University, 26(1): 14–19 (in Chinese)Google Scholar
  20. Wei C L, Jiang C J, Tao H Z, Wan X C (2004). Site-directed mutation of violaxanthin de-epoxidase from tea plant (Camellia sinensis) in vitro and expression bio-activity assay of the mutants. Chinese Journal of Biochemistry and Molecular Biology, 20(1): 73–78 (in Chinese)Google Scholar
  21. Wu S, Liang Y R, Lu J L, Li H Y (2005). Combination of particle bombardment-mediated and Agrobacterium-mediated transformation methods in tea plant. Journal of Tea Science, 25(4): 255–264 (in Chinese)Google Scholar
  22. Zhao D, Liu Z S, Xi B (2001). Cloning and alignment of polyphenol oxidase cDNA of tea plant. Journal of Tea Science, 21(2): 94–98 (in Chinese)Google Scholar
  23. Zhao L P, Chen L, Wang X C, Yao M Z (2006). Quantitative detection of β-glucosidase and β-primeverosidase gene expressions in different leaves of tea plant (Camellia sinensis) by real-time PCR analysis. Journal of Tea Science, 26(1): 11–16 (in Chinese)Google Scholar

Copyright information

© Higher Education Press and Springer-Verlag 2007

Authors and Affiliations

  1. 1.Lab for Tea Germplasm, Genetic Improvement and Molecular Biology, Tea Research InstituteChinese Academy of Agricultural SciencesHangzhouChina

Personalised recommendations