Synthesis and characterization of new azo-dye reagent and using to spectrophotometric determination of samarium(III) in some industrial and blood samples

  • Badr Abd El-wahaabEmail author
  • Khaled Elgendy
  • Akram El-didamony
Original Paper


A new azo-dye reagent was prepared by the reaction between sulfacetamide and rutin compounds. The new synthesized reagent of (E)-N-((4-((5-(5,7-dihydroxy-3-3-[3-α-l-rhamnopyranosyl-(1 → 6)-β-d-glucopyrano-syloxy]-4-oxo-4H-chromen-2-yl)-2,3-dihydroxyphenyl)diazenyl)phenyl)sulfonyl)acetamide [NDRGA] was characterized by FT-IR, 1HNMR, mass spectra and elemental analysis measurements and then used for the spectrophotometric determination of Sm(III). The proposed method was based on the formation of a lemon-colored complex between Sm(III) and NDGRA reagent in an alkaline medium using borate buffer at pH = 8 with absorption maximum at 475 nm. The method was enhanced by the use of cationic surfactant of cetylpyridinium bromide (CPB). Different factors affecting the formation and stability of the complex such as reagent concentration, time, temperature, solvents and order of addition were also studied. The composition of the complex was found to be 1:1 (metal: ligand) by using Job’s and molar ratio methods. The stability constant of the complex was calculated to be 1.1805 × 106. The method showed a good linearity in the concentration range of 2.0–90 µg ml−1 of Sm(III) with molar absorptivity and Sandell’s sensitivity 1.3014 × 104 L mol−1 cm−1 and 1.155 × 10−2 µg cm−2, respectively. The limit of quantification (LOQ) and detection (LOD) were calculated. The interference effect of some foreign ions was also studied. The validity of the calibration curve was found useful for the determination of micro-amounts of Sm(III) in some industrial and blood samples.


Spectrophotometry Samarium(III) Azo-dye reagent Beer’s law Stability constant Industrial and blood samples 



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Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2019

Authors and Affiliations

  • Badr Abd El-wahaab
    • 1
    Email author
  • Khaled Elgendy
    • 1
  • Akram El-didamony
    • 1
  1. 1.Chemistry Department, Faculty of ScienceZagazig UniversityZagazigEgypt

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