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Enhancement of erythritol production in Trichosporonoides oedocephalis by regulating cellular morphology with betaine

  • Pei Kang
  • Liangzhi LiEmail author
  • Lishi Yan
  • Xin Ju
  • Cuiying Hu
  • Xuemei Yao
Original Paper
  • 18 Downloads

Abstract

Erythritol is a four-carbon sugar alcohol produced by microorganisms and is used as a natural sweetener in food industry. The present study aimed to examine the impact of various surfactants, including betaine, Tween 20, Tween 80, Span 20, and Triton X-100, on the production of erythritol by Trichosporonoides oedocephalis ATCC 16958. Finally, after 120 h culture via agitation, the supplementation with 0.5 g/L betaine increased the erythritol production by 50.38%, which was best among the five surfactants. Therefore, betaine was chosen for further research. Subsequently, the activity of erythrose reductase (ER) with the addition of 0.5 g/L betaine (0.211 U/mg protein) was slightly higher than that of the culture without betaine (0.192 U/mg protein) at 48 h of culture. However, no significant changes were observed in the ER activity with betaine addition during the fermentation stages. Interestingly, further observation with scanning electron microscope indicated that the disruption of cell was found in the culture without betaine, while that of the culture with 0.5 g/L betaine addition remained intact at 120 h post-cultivation. The enhanced erythritol production with betaine addition affected the cellular morphology that could be regulated effectively by zwitterionic surfactant betaine. Finally, the scale-up fermentation in 5-L bioreactor, erythritol production with initial 0.5 g/L betaine and 30 mg/L CuSO4·5H2O addition was up to 59.34 g/L, which was 19.59% higher than the control with only 30 mg/L CuSO4·5H2O supplementation (49.62 g/L). Thus, the present study demonstrated the mechanisms underlying the betaine-affected erythritol production and provided a feasible approach for improving the erythritol production by T. oedocephalis.

Keywords

Betaine Cellular morphology Erythritol Trichosporonoides oedocephalis Zwitterionic surfactant 

Notes

Acknowledgements

The authors are grateful for the financial support from the National Natural Science Foundation of China (Grant No: 21376156, Grant No: 21676173). This study was also supported by Qing Lan Project of Jiangsu Education department. The authors are grateful for the financial support from the agricultural infrastructure project of Suzhou science and technology development plan (SNG2018046).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This article does not contain any studies with human or animal subjects performed by any of the authors.

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Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2019

Authors and Affiliations

  1. 1.School of Chemistry, Biology, and Material EngineeringSuzhou University of Science and TechnologySuzhouPeople’s Republic of China

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