Chemical Papers

, Volume 73, Issue 1, pp 151–158 | Cite as

Online solid-phase extraction liquid chromatography–mass spectrometry of hair cortisol using a surrogate analyte

  • Katarina Kostolanska
  • Lucie Novotna
  • Eva Taborska
  • Ondrej PesEmail author
Original Paper


Online SPE LC–MS with 13C3-cortisol as a surrogate analyte was developed, validated and applied for obtaining hair cortisol levels of 114 healthy volunteers. The first 3 cm from a posterior vertex position were analyzed as three individual segments resulting in 310 hair cortisol levels, which were used, after log transformation, to predict a range of values observed in future. The median value of hair cortisol was 4.76 pg/mg. The employed method allowed simple processing, high throughput and may be readily expanded to analyze additional steroid compounds in hair.


Endogenous cortisol Long-term hair concentration Surrogate analyte LC–MS Online SPE 



This work was supported by the Specific University Research Grant (MUNI/A/0910/2017) provided by the Ministry of Education, Youth and Sports of the Czech Republic in the year 2017.


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Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2018

Authors and Affiliations

  1. 1.Department of Biochemistry, Faculty of MedicineMasaryk UniversityBrnoCzech Republic

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