Online solid-phase extraction liquid chromatography–mass spectrometry of hair cortisol using a surrogate analyte
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Online SPE LC–MS with 13C3-cortisol as a surrogate analyte was developed, validated and applied for obtaining hair cortisol levels of 114 healthy volunteers. The first 3 cm from a posterior vertex position were analyzed as three individual segments resulting in 310 hair cortisol levels, which were used, after log transformation, to predict a range of values observed in future. The median value of hair cortisol was 4.76 pg/mg. The employed method allowed simple processing, high throughput and may be readily expanded to analyze additional steroid compounds in hair.
KeywordsEndogenous cortisol Long-term hair concentration Surrogate analyte LC–MS Online SPE
This work was supported by the Specific University Research Grant (MUNI/A/0910/2017) provided by the Ministry of Education, Youth and Sports of the Czech Republic in the year 2017.
- Binz TM, Braun U, Baumgartner MR, Kraemer T (2016) Development of an LC–MS/MS method for the determination of endogenous cortisol in hair using C-13(3)-labeled cortisol as surrogate analyte. J Chromatogr B Anal Technol Biomed Life Sci 1033:65–72. https://doi.org/10.1016/j.jchromb.2016.07.041 CrossRefGoogle Scholar
- Binz TM, Rietschel L, Streit F, Hofmann M, Gehrke J, Herdener M, Quednow BB, Martin NG, Rietschel M, Kraemer T, Baumgartner MR (2018) Endogenous cortisol in keratinized matrices: systematic determination of baseline cortisol levels in hair and the influence of sex, age and hair color. Forensic Sci Int 284:33–38. https://doi.org/10.1016/j.forsciint.2017.12.032 CrossRefGoogle Scholar
- Taieb J, Benattar C, Birr AS, Lindenbaum A (2002) Limitations of steroid determination by direct immunoassay. Clin Chem 48:583–585Google Scholar