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Chemical Papers

, Volume 72, Issue 10, pp 2561–2574 | Cite as

Validated and rapid measurement of the ferric reducing antioxidant power in plasma samples

  • Maria L. Gonzalez-Rivera
  • Flavio Martinez-Morales
  • Angel J. Alonso-Castro
  • Juan F. Lopez-Rodriguez
  • Juan R. Zapata-Morales
  • Saray Aranda Romo
  • Othoniel H. Aragon-Martinez
Original Paper
  • 20 Downloads

Abstract

The ferric reducing antioxidant power assay was developed using rat plasma samples and validated according to the Food and Drug Administration guidelines as well as strategies for the lack of endogenous compounds-free samples of matrix. For validation procedures, the phosphate buffered saline solution was used as the artificial matrix because this led to a direct interpolation in calibration curves. The absorbance responses and antioxidant activities in curves showed a second order polynomial relationship (R2 value = 0.9982). The precision, accuracy, and stability of the method ranged from 1.7 to 7.2%, 89.8 to 100.0%, and 82.7 to 111.6%, respectively. This assay had a short time of analysis (96 samples per min) and absence of interferences during the spectrophotometric monitoring. For the application of the method, the plasma antioxidant capacity, blood distribution of levofloxacin, and biometry hematic were evaluated in samples obtained from rats under different experimental conditions. The in vitro condition applied to blood samples increased the plasma antioxidant capacity and volume of erythrocytes, whereas diminished the levofloxacin concentration in these cells. The high antioxidant activity was produced by a high amount of inosine, which in turn was caused by high oxidative stress leading to an impaired blood distribution of levofloxacin and erythrocyte swelling. This assay is a validated and rapid biomarker for the evaluation of the total antioxidant capacity in plasma samples.

Graphical abstract

Keywords

Ferric reducing antioxidant power Bioanalytical validation Surrogate matrix Erythrocyte dysfunction Fluoroquinolone 

Abbreviations

ACK

Acesulfame potassium

bw

Body weight

FDA

Food and Drug Administration

FRAP

Ferric reducing antioxidant power (µmol Fe(II) L−1)

LC

Liquid chromatography

LLOQ

Lower limit of quantification (µmol Fe(II) L−1)

LVX

Levofloxacin

MCHC

Mean corpuscular hemoglobin concentration (g dL−1)

MCV

Mean corpuscular volume (fL)

PBS

Phosphate buffered saline

QC

Quality control

R2

Coefficient of determination

TPTZ

2,4,6-Tris(2-pyridyl)-s-triazine

Notes

Acknowledgements

This work was supported by Scientific Research Funding obtained from the Autonomous University of San Luis Potosi (Grant Number C16-FAI-09-32.32). Maria L. Gonzalez-Rivera is a CONACYT fellow (Grant Number: 584981).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Institute of Chemistry, Slovak Academy of Sciences 2018

Authors and Affiliations

  • Maria L. Gonzalez-Rivera
    • 1
  • Flavio Martinez-Morales
    • 1
  • Angel J. Alonso-Castro
    • 2
  • Juan F. Lopez-Rodriguez
    • 3
  • Juan R. Zapata-Morales
    • 2
  • Saray Aranda Romo
    • 4
  • Othoniel H. Aragon-Martinez
    • 1
    • 5
  1. 1.Department of Pharmacology, School of MedicineAutonomous University of San Luis PotosiSan Luis PotosiMexico
  2. 2.Division of Natural and Exact Sciences, Department of PharmacyUniversity of GuanajuatoGuanajuatoMexico
  3. 3.Animal Laboratory, School of MedicineAutonomous University of San Luis PotosiSan Luis PotosiMexico
  4. 4.Diagnostic Clinic, Dentistry SchoolAutonomous University of San Luis PotosíSan Luis PotosiMexico
  5. 5.Laboratory of Natural Compounds (LABCON)San Luis PotosiMexico

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