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Gastric Bypass Improves Obesity and Glucose Tolerance Independent of Gastric Pouch Size

  • Rui Xu
  • Chenyu Zhu
  • Joseph F. PierreEmail author
  • Deng Ping YinEmail author
Original Contributions
  • 46 Downloads

Abstract

Purpose

We investigated whether metabolic phenotype improvements following gastric bypass are associated with gastric resection strategy in high-fat diet-induced obese (DIO) mice.

Materials and Methods

We developed the mouse Roux-en-Y gastric bypass (RYGB) model with different gastric pouch sizes: (i) RYGB with a large gastric pouch (RYGB-LP), where the stomach was transected, and the jejunum was anastomosed to the residual forestomach, in which 30% of the stomach is retained. (ii) RYGB with a small remnant gastric pouch (RYGB-SP), where the stomach was transected 0.8 cm distal to the esophagogastric junction, and the jejunum is attached to a small remnant of the forestomach (~ 10% of the stomach). (iii) RYGB without gastric pouch (RYGB-NP), where the jejunum is anastomosed to the lower portion of the esophagus.

Results

Surgical success rate (or 4-week mouse survival rate) of the RYGB-LP, RYGB-SP, and RYGB-NP procedures was 50, 75, and 85%, respectively. Our data demonstrate that all RYGB procedures improved body weight, glucose tolerance, and liver steatosis, compared with untreated DIO mice at 8-week post-surgery. Major surgical complication, such as obstruction at the forestomach, occurred predominantly in RYGB-LP mice, resulting in a higher mortality. Pre- and post-prandial plasma ghrelin levels did not correlate with improved metabolic phenotype after gastric bypass.

Conclusions

We conclude that RYGB with different gastric pouch equally improves obesity and glucose tolerance independent of gastric pouch size and total plasma ghrelin levels in the mouse model of RYGB surgery.

Keywords

Gastric Sleeve Bariatric surgery Metabolic surgery Ghrelin 

Notes

Funding Information

This work was supported in part by the CTSA grant (CTSA TR000430 at the University of Chicago) to DPY and NIH grant DK020595 to the Metabolic Core.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Statement of Animal Rights/Ethical Approval

All experiments and surgical preparations were performed according to the protocol approved by the University of Chicago Institutional Animal Care and Use Committee (IACUC).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of SurgeryYichang Central Hospital & Three Gorges University First Clinical CollegeYichangChina
  2. 2.Department of PediatricsUniversity of Tennessee Health Science CenterMemphisUSA
  3. 3.Animal Microsurgery Center, Department of SurgeryUniversity of Chicago School of MedicineChicagoUSA

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