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Obesity Surgery

, Volume 29, Issue 9, pp 2745–2749 | Cite as

Duodenal-Jejunal Bypass Maintains Gut Permeability by Suppressing Gut Inflammation

  • Hideya KashiharaEmail author
  • Mitsuo Shimada
  • Kozo Yoshikawa
  • Jun Higashijima
  • Tomohiko Miyatani
  • Takuya Tokunaga
  • Masaaki Nishi
  • Chie Takasu
Brief Communication
  • 107 Downloads

Abstract

Background

The aim of this study was to investigate gut inflammation and permeability in rats after duodenal-jejunal bypass (DJB) and in rats injected with a glucagon-like peptide-1 (GLP-1) receptor analog.

Methods

Twelve male 16-week-old obese diabetic rats were divided into three groups: the DJB group, the sham group, and the group injected daily with a GLP-1 receptor agonist (liraglutide). Gut inflammation and the expression of tight junction protein (claudin-1) were analyzed in the three groups at 8 weeks after surgery.

Results

The DJB group showed significantly lower levels of gut inflammatory cytokines than the liraglutide group. Claudin-1 showed stronger intensity on immunofluorescent staining in the DJB group than that in the liraglutide group.

Conclusions

In summary, DJB surgery might maintain gut permeability via suppression of gut inflammation.

Keywords

Gut inflammation Gut permeability Tight junction protein (claudin-1) GLP-1 receptor agonist (liraglutide) Gut inflammatory cytokines (IFN-γ, IL-1, IL-6, and TNF-α) Roux-en-Y reconstruction Bariatric surgery 

Abbreviations

DJB

Duodenal-jejunal bypass

GLP-1

Glucagon-like peptide-1

HFD

High-fat diet

RNA

Ribonucleic acid

RT

Reverse transcription

PBS

Phosphate-buffered salts

DAPI

4′,6-Diamidino-2-phenylindole

LPS

Lipopolysaccharide

NASH

Nonalcoholic steatohepatitis

Notes

Compliance with Ethical Standards

All applicable institutional and/or national guidelines for the care and use of animals were followed. Informed consent statement does not apply to this study.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of SurgeryTokushima UniversityTokushimaJapan

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