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Obesity Surgery

, Volume 29, Issue 3, pp 882–890 | Cite as

The Changes of Serum Metabolites in Diabetic GK Rats after Ileal Transposition Surgery

  • Kemin Yan
  • Weijie Chen
  • Huijuan Zhu
  • Guole Lin
  • Wei Sun
  • Xiaoyan Liu
  • Hui Pan
  • Linjie Wang
  • Hongbo Yang
  • Meijuan Liu
  • Fengying GongEmail author
Original Contributions

Abstract

Background

Ileal transposition (IT) surgery could improve metabolism. Metabolomics has been applied comprehensively in analyzing the global dynamic alterations of metabolites. In the present study, we aimed to investigate serum metabolite alterations in diabetic Goto-Kakizaki (GK) rats after IT surgery.

Methods

Male GK rats were subjected to IT and Sham-IT surgery. Six weeks later, body weight, food intake, fat mass, and serum biochemical parameters were measured. The serum metabolomic fingerprint was analyzed using ultra-performance liquid chromatography–mass spectrometry (LC-MS)-based, non-targeted metabolomic approach. The differential metabolites were identified using principal component analysis and orthogonal partial least squares discriminant analysis. Metabolic pathway analysis was performed using HMDB and KEGG databases.

Results

The body weight, food intake, fat mass, serum levels of glucose and insulin, and homeostasis model assessment of insulin resistance (HOMA-IR) of IT rats were significantly decreased when compared with Sham-IT rats (all P < 0.05). In the metabolomics analysis, ten serum differential metabolites were identified. Compared with Sham-IT rats, serum LysoPC(O-18:0) and PG(20:4/20:0) of IT rats were decreased, while genistein 4′-O-glucuronide, 5,6:8,9-Diepoxyergost-22-ene-3,7beta-diol, PI(16:0/18:2(9Z,12Z)), docosapentaenoic acid, 3-Oxo-4,6-choladienoic acid, 3-Oxocholic acid, and TG were increased. Pathway analysis highlighted the following pathways: ether lipid metabolism, alpha linolenic acid and linolenic acid metabolism, incretin synthesis and secretion, free fatty acid receptors, and biosynthesis of unsaturated fatty acids.

Conclusions

IT surgery could significantly decrease body weight and fat mass and improve glucose metabolism in diabetic GK rats. These beneficial effects might be related to the changes of serum metabolites which involved in lipid metabolism, bile acids, and incretin.

Keywords

Ileal transposition (IT) surgery Metabolomics Fat mass Liquid chromatography–mass spectrometry (LC-MS) Goto-Kakizaki (GK) rats 

Notes

Funding

The study was supported by grants from the National Natural Science Foundation of China (No. 81400797 for Weijie Chen, No. 30600836, 81471024 for Huijuan Zhu, No. 30540036, 30771026, 81370898 for Fengying Gong), the Beijing Natural Science Foundation (No. 7082079, 7182130 for Fengying Gong), the National Key Program of Clinical Science (WBYZ2011-873 for Fengying Gong and Huijuan Zhu), and the PUMCH Foundation (2013-020 for Fengying Gong).

Compliance with Ethical Standards

All animal experimental procedures were approved by the ethics committee of Peking Union Medical College Hospital.

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

11695_2018_3582_MOESM1_ESM.pdf (121 kb)
Figure S1 (PDF 120 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Endocrinology of National Health Commission, Department of EndocrinologyPeking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical CollegeBeijingChina
  2. 2.Department of Surgery, Peking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijingChina
  3. 3.Core Facility of Instrument, Institute of Basic Medical SciencesChinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical CollegeBeijingChina

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