A Screening Study of Potential Carcinogen Biomarkers After Surgical Treatment of Obesity
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Obesity has been associated with the development of various types of cancer. Biomarker studies may provide molecular level knowledge of the factors involved in this association, improving clinical practice through new methods of prevention and treatment.
The present study aimed to analyze proteins found in the plasma of obese patients prior to and 6 months after bariatric surgery, using body mass index (BMI) and percentage total weight loss (%TWL) to evaluate, in a prospective manner, the effects of weight loss on the regulation of proteins related to the appearance of tumors.
Material and Methods
This was a cohort study designed to compare parameters before and after intervention. A total of 40 patients were divided into two groups: control (n = 10) and obese (n = 30). The latter group was stratified according to surgical technique used (Roux-en-Y gastric bypass (RYGB) n = 11 and sleeve gastrectomy (SG) n = 19) to remove confounding variables. Blood samples were collected for plasma protein studies using two-dimensional electrophoresis.
Six proteins related to carcinogenesis were hyperexpressed in the obese patients but were absent in the control group and following surgery. These proteins were the beta-receptor of derived growth factor platelet, the receptor of apolipoprotein B, thrombospondin-2, the low-density lipoprotein receptor, transthyretin, and podoplanin.
The current preliminary study thus identified potentially carcinogenic proteins in obese patients. Surgical weight loss resulted in the not detection of these proteins.
KeywordsObesity Cancer Biomarkers Proteomics Two-dimensional electrophoresis Bariatric surgery
The authors thank Dr. Djalma A. de Melo who participated in the statistical analysis of this work.
Luciana Teixeira de Siqueira is the principal researcher responsible for patients study at the Hospital das Clinicas of Federal University of Pernambuco. Marcela Silvestre Outtes Wanderley is responsible for the proteomic study. Roberto Afonso da Silva is responsible for the evaluation of potential biomarkers. Adriana da Silva Andrade Pereira is responsible for the two-dimensional electrophoresis.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no competing interests.
All procedures performed were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants included in the study.
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