Obesity Surgery

, Volume 24, Issue 9, pp 1567–1571 | Cite as

The Effect of PPI Use on Human Gut Microbiota and Weight Loss in Patients Undergoing Laparoscopic Roux-en-Y Gastric Bypass

  • Emily K. Ward
  • Dara P. Schuster
  • Katie H. Stowers
  • Amanda K. Royse
  • Diana Ir
  • Charles E. Robertson
  • Daniel N. Frank
  • Gregory L. Austin
Brief Communication


Laparoscopic Roux-en-Y gastric bypass (LRYGB) achieves sustainable weight loss possibly by altering the gut microbiota. The effect of a proton pump inhibitor (PPI) on weight loss and the gut microbiota has not been explored. PPI use and the gut microbiota were assessed before and 6 months after LRYGB in eight patients. Bacterial profiles were generated by 16S ribosomal RNA (rRNA) gene sequencing. Prior to LRYGB, PPI users had a higher percent relative abundance (PRA) of Firmicutes compared to nonusers. PPI users at 6 months post-LRYGB had a higher PRA of Firmicutes [48.6 versus 35.6 %, p = nonsignificant (NS)] and a trend toward significantly lower percent excess weight loss (49.3 versus 61.4 %, p = 0.067) compared to nonusers. PPI use post-LRYGB may impair weight loss by modifying gut microbiota.


PPI Severe obesity Gut microbiota Gastric bypass 



Percent relative abundance


Proton pump inhibitor


Roux-en-Y gastric bypass


Laparoscopic Roux-en-Y gastric bypass


Percent weight loss


Percent excess weight loss





This project was supported by a grant from the Colorado Clinical Translational Science Institute Project Grant (5UL1RR025780).

Conflict of Interest

No conflicts of interest exist for any of the authors.


No writing assistance was provided for this manuscript.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Emily K. Ward
    • 1
  • Dara P. Schuster
    • 2
    • 3
  • Katie H. Stowers
    • 2
  • Amanda K. Royse
    • 2
  • Diana Ir
    • 4
  • Charles E. Robertson
    • 5
  • Daniel N. Frank
    • 4
  • Gregory L. Austin
    • 1
  1. 1.Division of Gastroenterology and HepatologyUniversity of Colorado DenverAuroraUSA
  2. 2.Division of Endocrinology, Diabetes, and MetabolismThe Ohio State University Wexner Medical CenterColumbusUSA
  3. 3.Center for Minimally Invasive SurgeryThe Ohio State University Wexner Medical CenterColumbusUSA
  4. 4.Division of Infectious Diseases and Microbiome Research Consortium (MiRC)University of Colorado DenverAuroraUSA
  5. 5.Department of Molecular, Cellular, and Developmental BiologyUniversity of ColoradoBoulderUSA

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