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Obesity Surgery

, Volume 23, Issue 9, pp 1436–1444 | Cite as

Duodenal–Jejunal Bypass Improves Glucose Metabolism and Adipokine Expression Independently of Weight Loss in a Diabetic Rat Model

  • Chunxiao Hu
  • Guangyong Zhang
  • Dong Sun
  • Haifeng Han
  • Sanyuan HuEmail author
Original Contributions

Abstract

Background

There is accumulating evidence that adipokines lead to a proinflammatory state, which plays crucial roles in insulin resistance and development of type 2 diabetes mellitus (T2DM). Previous studies demonstrated that weight loss after bariatric surgery is accompanied by a suppression of the proinflammatory state. However, the effect of bariatric surgery on adipokine expression beyond weight loss is still elusive. The aim of this study was to investigate the effect of duodenal–jejunal bypass (DJB) on glucose homeostasis and adipokine expression independently of weight loss.

Methods

A T2DM rat model was developed by a high-fat diet and low dose of streptozotocin. Twenty-one diabetic rats and 10 age-matched SD rats were randomly assigned to the DJB group, sham-DJB (S-DJB) group, and control group. For 12 weeks after surgery, their body weight, food intake, glucose homeostasis, lipid parameters, serum adipokine levels, and adipokine gene expression in the mesocolon adipose tissue were measured.

Results

Compared to the S-DJB group, DJB induced significant and sustained glycemic control with improved insulin sensitivity and glucose tolerance independently of weight loss. DJB improved the lipid metabolism by decreasing fasting free fatty acids and triglycerides. Serum leptin and IL-6 significantly decreased 12 weeks after DJB, whereas adiponectin increased and TNF-α remained unchanged. The mRNA expression levels of leptin, TNF-α, and IL-6 decreased, whereas adiponectin increased in the mesocolon adipose tissue.

Conclusion

DJB reduced the proinflammatory adipokines and increased the anti-inflammatory adipokines independently of weight loss, which may contribute to the improvement of insulin sensitivity.

Keywords

Duodenal–jejunal bypass Type 2 diabetes mellitus Adipokine High-fat diet Streptozotocin 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (no. 81270888/H0713 and no. 81070642/H0713), Specialized Research Fund for the Doctoral Program of Higher Education (no. 20100131110049), Shandong Provincial Outstanding Medical Academic Professional Program, Natural Science Foundation of Shandong Province grants (no. ZR2012HQ030), and Graduate Independent Innovation Foundation of Shandong University (no.yzc12160). Thanks to Dr. Edward C. Mignot (Shandong University) and EDANZ (a professional language service) for linguistic advice.

Conflict of Interest

The authors have nothing to disclose.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Chunxiao Hu
    • 1
  • Guangyong Zhang
    • 1
  • Dong Sun
    • 1
  • Haifeng Han
    • 1
  • Sanyuan Hu
    • 1
    Email author
  1. 1.Department of General SurgeryQilu Hospital of Shandong UniversityJinanPeople’s Republic of China

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