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Obesity Surgery

, Volume 23, Issue 4, pp 554–560 | Cite as

Fibronectin Gene Expression in Human Adipose Tissue and Its Associations with Obesity-Related Genes and Metabolic Parameters

  • Seoung Hee Lee
  • Hye Soon Park
  • Jung Ah Lee
  • Young Sook Song
  • Yeon Jin Jang
  • Jong-Hyeok Kim
  • Yeon Ji Lee
  • Yoonseok Heo
Clinical Research

Abstract

Background

Limited data are available on the in vivo expression of fibronectin, one of the main extracellular matrix components. We investigated the expression of fibronectin in abdominal visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) and the associations of leptin, adiponectin, and vaspin gene expression with metabolic parameters in obese women.

Methods

We recruited female subjects undergoing bariatric surgery for obesity (n = 24) and patients undergoing benign gynecological surgery as the control group (n = 23). We measured anthropometric variables, abdominal fat distribution, metabolic parameters, serum concentrations of leptin, adiponectin, and vaspin, and depot-specific mRNA expression of fibronectin, leptin, adiponectin, and vaspin.

Results

Fibronectin expression in both VAT and SAT was significantly lower in the obese group than in the control group. Fibronectin expression in both VAT and SAT were negatively correlated with body mass index or waist circumference, with higher prominence in VAT. In multiple regression analysis, fibronectin expression in both VAT and SAT was negatively correlated with serum leptin concentration. Fibronectin expression in VAT was negatively correlated with leptin expression in VAT. Additionally, fibronectin expression in SAT was negatively correlated with leptin expression in SAT and positively correlated with adiponectin expression in VAT and SAT.

Conclusions

We found significant negative associations between depot-specific fibronectin expression in human adipose tissue and obesity indices and obesity-related biomarkers. Our results suggest that fibronectin expression may contribute to obesity and metabolic dysregulation in humans.

Keywords

Obesity Adipose tissue Fibronectin Leptin Adiponectin 

Notes

Conflict of Interest

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science, and Technology (2012–004869) Seoul, Korea. Seoung Hee Lee, Hye Soon Park, Jung Ah Lee, Young Sook Song, Yeon Jin Jang, Jong-Hyeok Kim, Yeon Ji Lee, Yoonseok Heo declare that they have no conflicts of interest.

References

  1. 1.
    Adams JC, Watt FM. Fibronectin inhibits the terminal differentiation of human keratinocytes. Nature. 1989;340:307–9.PubMedCrossRefGoogle Scholar
  2. 2.
    Rutkowski JM, Davis KE, Scherer PE. Mechanisms of obesity and related pathologies: the macro- and microcirculation of adipose tissue. FEBS J. 2009;276:5738–46.PubMedCrossRefGoogle Scholar
  3. 3.
    Pasarica M, Gowronska-Kozak B, Burk D, et al. Adipose tissue collagen VI in obesity. J Clin Endocrinol Metab. 2009;94:5155–62.PubMedCrossRefGoogle Scholar
  4. 4.
    Divoux A, Tordjman J, Lacasa D, et al. Fibrosis in human adipose tissue: composition, distribution, and link with lipid metabolism and fat mass loss. Diabetes. 2010;59:2817–25.PubMedCrossRefGoogle Scholar
  5. 5.
    Pankov R, Yamada KM. Fibronectin at a glance. J Cell Sci. 2002;115:3861–3.PubMedCrossRefGoogle Scholar
  6. 6.
    Antras J, Hilliou F, Redziniak G, et al. Decreased biosynthesis of actin and cellular fibronectin during adipose conversion of 3T3–F442A cells. Reorganization of the cytoarchitecture and extracellular matrix fibronectin. Biol Cell. 1989;66:247–54.PubMedGoogle Scholar
  7. 7.
    Pierleoni C, Verdenelli F, Castellucci M, et al. Fibronectins and basal lamina molecules expression in human subcutaneous white adipose tissue. Eur J Histochem. 1998;42:183–8.PubMedGoogle Scholar
  8. 8.
    Taleb S, Cancello R, Clement K, et al. Cathepsin s promotes human preadipocyte differentiation: possible involvement of fibronectin degradation. Endocrinology. 2006;147:4950–9.PubMedCrossRefGoogle Scholar
  9. 9.
    Yoshizumi T, Nakamura T, Yamane M, et al. Abdominal fat: standardized technique for measurement at CT. Radiology. 1999;211:283–6.PubMedGoogle Scholar
  10. 10.
    Matthews DR, Hosker JP, Rudenski AS, et al. Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia. 1985;28:412–9.PubMedCrossRefGoogle Scholar
  11. 11.
    Novak M, Monkus E, Pardo V, et al. Preparation of subcellular fractions suitable for biochemical analyses from human subcutaneous adipose tissue obtained by needle biopsy. I. Isolation of mitochondria on a microscale. Exp Cell Res. 1972;73:335–44.PubMedCrossRefGoogle Scholar
  12. 12.
    Rodriguez Fernandez JL, Ben-Ze'ev A. Regulation of fibronectin, integrin and cytoskeleton expression in differentiating adipocytes: inhibition by extracellular matrix and polylysine. Differentiation. 1989;42:65–74.PubMedCrossRefGoogle Scholar
  13. 13.
    Spiegelman BM, Ginty CA. Fibronectin modulation of cell shape and lipogenic gene expression in 3T3–adipocytes. Cell. 1983;35:657–66.PubMedCrossRefGoogle Scholar
  14. 14.
    Wang Y, Zhao L, Smas C, et al. Pref-1 interacts with fibronectin to inhibit adipocyte differentiation. Mol Cell Biol. 2010;30:3480–92.PubMedCrossRefGoogle Scholar
  15. 15.
    Hsieh P, Chen LB. Behavior of cells seeded in isolated fibronectin matrices. J Cell Biol. 1983;96:1208–17.PubMedCrossRefGoogle Scholar
  16. 16.
    Yamada KM, Yamada SS, Pastan I. Quantitation of a transformation-sensitive, adhesive cell surface glycoprotein. Decrease of several untransformed permanent cell lines. J Cell Biol. 1977;74:649–54.PubMedCrossRefGoogle Scholar
  17. 17.
    Spiegelman BM, Green H. Control of specific protein biosynthesis during the adipose conversion of 3T3 cells. J Biol Chem. 1980;255:8811–8.PubMedGoogle Scholar
  18. 18.
    Miller RE, Carrino DA. An association between glutamine synthetase activity and adipocyte differentiation in cultured 3T3–L1 cells. Arch Biochem Biophys. 1981;209:486–503.PubMedCrossRefGoogle Scholar
  19. 19.
    Steinberg MM, Brownstein BL. A clonal analysis of the differentiation of 3T3–L1 preadipose cells: role of insulin. J Cell Physiol. 1982;113:359–64.PubMedCrossRefGoogle Scholar
  20. 20.
    Li Q, Hosaka T, Jambaldorj B, et al. Extracellular matrix with the rigidity of adipose tissue helps 3T3–L1 adipocytes maintain insulin responsiveness. J Med Investig. 2009;56:142–9.CrossRefGoogle Scholar
  21. 21.
    Zhang W, Ambati S, Della-Fera MA, et al. Leptin modulated changes in adipose tissue protein expression in ob/ob mice. Obesity (Silver Spring). 2011;19:255–61.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Seoung Hee Lee
    • 1
  • Hye Soon Park
    • 1
  • Jung Ah Lee
    • 1
  • Young Sook Song
    • 2
  • Yeon Jin Jang
    • 2
  • Jong-Hyeok Kim
    • 3
  • Yeon Ji Lee
    • 4
  • Yoonseok Heo
    • 5
  1. 1.Department of Family MedicineUniversity of Ulsan College of MedicineSeoulSouth Korea
  2. 2.Department of PhysiologyUniversity of Ulsan College of MedicineSeoulSouth Korea
  3. 3.Department of Obstetrics and GynecologyUniversity of Ulsan College of MedicineSeoulSouth Korea
  4. 4.Department of Family MedicineInha University, College of MedicineIncheonSouth Korea
  5. 5.Department of General SurgeryInha University, College of MedicineIncheonSouth Korea

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