Obesity Surgery

, Volume 22, Issue 7, pp 1110–1116 | Cite as

Sleeve Gastrectomy Improves Glucose Homeostasis in Zucker Diabetic Fatty Rats

  • Jean Christophe Lifante
  • Luca Milone
  • Judith Korner
  • Gift Kopsombut
  • Manu Sebastian
  • William B. InabnetIII
Animal Research
First Online:

Abstract

Background

Bariatric surgery improves glucose homeostasis, but the mechanism of action is poorly understood. The aim of this study was to assess the effect of sleeve gastrectomy (SG) on glucose homeostasis in two obese populations of rats.

Methods

Two strains of rats [Zucker fatty (ZF) and Zucker diabetic fatty (ZDF)] were each divided into two groups: sham and SG. Food intake was measured daily, and weight was measured bi-weekly. Oral glucose tolerance testing (OGTT) was performed before and 45 days after surgery.

Results

In both strains of rats, there was no statistical difference in food intake and weight gain between the sham and SG rodents before and after surgery. In ZF rats, there was no change in fasting glucose or OGTT area under the curve (AUC) before or 45 days after surgery. In the ZDF rodents, the mean preoperative fasting glucose and OGTT AUC was 204 ± 25 and 25,441 ± 2,648, respectively. At 45 days after surgery, mean fasting glucose significantly increased in the sham (sham = 529 ± 26, p = 0.0003) but not in the SG rodents (SG = 289 ± 46, p = 0.1113). In ZDF sham animals, OGTT at 45 days showed a higher AUC compared to before surgery (44,983 ± 6,338, p = 0.006), whereas in ZDF SG rodents, the increase in AUC glucose approached but did not reach statistical significance (35,553 ± 3,925, p = 0.06).

Conclusions

In ZF and ZDF rodents, SG did not influence food intake and weight evolution. In ZDF rodents, diabetes progressed in the sham group but not in the SG group.

Keywords

Sleeve gastrectomy Obesity Diabetes Zucker fatty rats Diabetes surgery Glucose homeostasis 
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Notes

Conflicts of interest

J. C. Lifante, L. Milone, and M. Sebastian declare no conflicts of interest. W. B. Inabnet receives an educational grant from Covidien. J. Korner is a member of the Scientific Advisory Board of Nutrisystem, receives a research grant from Covidien and discretionary research fund from Columbia University, and is a consultant from Johnson & Johnson/Ethicon and Unigene.

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Copyright information

© Springer Science + Business Media, LLC 2012

Authors and Affiliations

  • Jean Christophe Lifante
    • 1
  • Luca Milone
    • 2
  • Judith Korner
    • 3
  • Gift Kopsombut
    • 4
  • Manu Sebastian
    • 5
  • William B. InabnetIII
    • 6
  1. 1.Department of General and Endocrine SurgeryCentre Hospitalier Universitaire Lyon SudPierre BéniteFrance
  2. 2.Department of Surgery, Division of Minimal Access and Bariatric SurgeryColumbia University College of Physicians and SurgeonsNew YorkUSA
  3. 3.Department of MedicineColumbia University College of Physicians and SurgeonsNew YorkUSA
  4. 4.Institute of NutritionColumbia University College of Physicians and SurgeonsNew YorkUSA
  5. 5.Department of Pathology and Cell BiologyColumbia UniversityNew YorkUSA
  6. 6.Division of Metabolic, Endocrine and Minimally Invasive Surgery, Department of Surgery, Mount Sinai School of MedicineNew YorkUSA

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