Obesity Surgery

, Volume 21, Issue 6, pp 774–782 | Cite as

Glycosaminoglycans of Abdominal Skin After Massive Weight Loss in Post-bariatric Female Patients

  • Daniela Francescato Veiga
  • Rodolpho A. Bussolaro
  • Elsa Y. Kobayashi
  • Valquíria P. Medeiros
  • João R. M. Martins
  • Elvio B. Garcia
  • Neil F. Novo
  • Helena B. Nader
  • Lydia M. Ferreira
Basic Science Research

Abstract

Background

The number of post-bariatric patients had a significant increase over the last years, and a better understanding of the consequences of massive weight loss on skin is imperative. Despite weight-loss-related changes in collagen and elastin have been reported, less is known about changes in another of the matrix components of the skin, the glycosaminoglycans. The objective of this study is to evaluate abdominal skin glycosaminoglycans concentrations and perlecan and collagen III expression in post-bariatric female patients.

Methods

Skin tissue samples from the abdomen of lean (n = 19) and post-bariatric (n = 24) female patients were compared. Sulfated glycosaminoglycans and hyaluronic acid were extracted, characterized and quantified. Perlecan and collagen III expression was assessed by immunofluorescence.

Results

The major glycosaminoglycans found were dermatan sultafe and hyaluronic acid; the others were found in smaller amounts. The skin of the post-bariatric patients had lower concentrations of heparan sulfate (p = 0.002) while hyaluronic acid, dermatan sulfate, and chondroitin sulfate concentrations were similar to the lean women’s skin. Post-bariatric skin showed decreased expression of perlecan and increased expression of collagen III. No correlation was found among glycosaminoglycans concentrations and age, body mass index, frequency of pregnancies, or skin types, but it was observed in higher skin heparan sulfate concentrations in post-bariatric patients who had their weights stabilized for over than 24 months (p = 0.000).

Conclusion

Abdominal skin of post-bariatric women presented decreased heparan sulfate concentrations and perlecan expression and increased expression of collagen III.

Keywords

Bariatric surgery Weight loss Women Skin Glycosaminoglycans 

Notes

Acknowledgments

This work was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 471468/2008-8).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science + Business Media, LLC 2011

Authors and Affiliations

  • Daniela Francescato Veiga
    • 1
    • 2
  • Rodolpho A. Bussolaro
    • 1
  • Elsa Y. Kobayashi
    • 3
  • Valquíria P. Medeiros
    • 3
  • João R. M. Martins
    • 3
    • 4
  • Elvio B. Garcia
    • 1
  • Neil F. Novo
    • 5
  • Helena B. Nader
    • 3
  • Lydia M. Ferreira
    • 1
  1. 1.Division of Plastic Surgery, Department of SurgeryUniversidade Federal de São PauloSão PauloBrazil
  2. 2.Division of Plastic Surgery, Department of SurgeryUniversidade do Vale do SapucaíPouso AlegreBrazil
  3. 3.Division of Molecular Biology, Department of BiochemistryUniversidade Federal de São PauloSão PauloBrazil
  4. 4.Division of Endocrinology and Metabolism, Department of MedicineUniversidade Federal de São PauloSão PauloBrazil
  5. 5.Department of BioestatisticsUniversidade do Vale do SapucaíPouso AlegreBrazil

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