Obesity Surgery

, Volume 21, Issue 5, pp 633–643 | Cite as

Expression Profile in Omental and Subcutaneous Adipose Tissue from Lean and Obese Subjects. Repression of Lipolytic and Lipogenic Genes

  • Carmen Hurtado del Pozo
  • Rosa María Calvo
  • Gregorio Vesperinas-García
  • Javier Gómez-Ambrosi
  • Gema Frühbeck
  • Miguel Angel Rubio
  • Maria Jesus Obregon


The adipose tissue is a highly regulated endocrine and paracrine organ that secretes a wide variety of biologically active molecules involved in the control of energy balance and the regulation of body weight. Our work aimed to analyze the dysregulation of the adipocyte metabolism and compare the gene expression patterns between omental (OM) and subcutaneous (SC) adipose tissue from obese and lean subjects by using whole-genome DNA microarrays. OM and SC adipose tissues were obtained from 43 obese subjects undergoing bariatric surgery and from six lean individuals. Gene expression analysis was performed by whole-genome microarrays and Taqman RT-PCR. The analysis of microarrays showed upregulation of 545 genes in OM and 47 in SC adipose tissue, whereas 723 and 27 genes were downregulated in OM and SC tissue, respectively, in obese patients. Significantly altered genes showed at least a twofold change of p < 0.05. Validation of the arrays with 28 genes was carried out by using low density microfluidic cards which confirmed the changes found in most genes. We focused on the altered expression of gene coding for enzymes and transcription factors involved in lipid metabolism. Interestingly, some of these genes have not been previously described in obesity. Our results show that adipose tissue from obese subjects entails defense mechanisms against an excessive expansion and fat accumulation, repressing both lipogenesis and lipolysis.


Obesity Subcutaneous adipose tissue Omental Whole-genome microarrays 


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Copyright information

© Springer Science + Business Media, LLC 2010

Authors and Affiliations

  • Carmen Hurtado del Pozo
    • 1
    • 2
  • Rosa María Calvo
    • 1
    • 2
  • Gregorio Vesperinas-García
    • 3
  • Javier Gómez-Ambrosi
    • 2
    • 4
  • Gema Frühbeck
    • 2
    • 4
  • Miguel Angel Rubio
    • 5
  • Maria Jesus Obregon
    • 1
    • 2
  1. 1.Instituto de Investigaciones Biomedicas Alberto SolsCSIC-UAMMadridSpain
  2. 2.CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN) ISCIIIMadridSpain
  3. 3.Servicio de CirugíaHospital Universitario “La Paz”MadridSpain
  4. 4.Metabolic Research laboratoryClínica Universidad de NavarraPamplonaSpain
  5. 5.Servicio de Endocrinología y NutriciónHospital Clínico S. CarlosMadridSpain

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