RIP140 Gene and Protein Expression Levels are Downregulated in Visceral Adipose Tissue in Human Morbid Obesity
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Receptor-interacting protein 140 (RIP140) is a corepressor for nuclear receptors with an important role in the inhibition of energy expenditure. Rip140-knockout mice are lean and resistant to diet-induced obesity due to an increase in mitochondrial biogenesis, fatty acid oxidation, and oxidative phosphorylation. The aim of the present work was to evaluate the effect of morbid obesity on gene and protein expression levels of RIP140 in visceral adipose tissue (VAT).
VAT biopsies obtained from 17 subjects were used in the study. Patients were classified as lean (body mass index [BMI] = 21.8 ± 1.3 kg/m2) or obese (BMI = 48.2 ± 2.6 kg/m2). Reverse transcription polymerase chain reaction and Western blot analyses were performed to quantify the expression levels of RIP140 in VAT. We also analyzed glucose and lipid profile as well as some inflammatory factors.
Obese patients exhibited significantly lower RIP140 mRNA expression levels compared to lean subjects (lean = 1.00 ± 0.17 arbitrary units, obese = 0.65 ± 0.18 arbitrary units; P < 0.05). Protein expression of RIP140 followed the same trend, being significantly higher in lean volunteers (lean = 1.00 ± 0.18 arbitrary units, obese = 0.45 ± 0.11 arbitrary units; P < 0.05). Furthermore, a significant negative correlation was found between RIP140 protein levels and both BMI (ρ = −0.85; P < 0.001) and body fat percentage (ρ = −0.88; P < 0.001).
The lower gene and protein expression levels of RIP140 in obese subjects may suggest a compensatory mechanism in order to favor energy expenditure and reduce fat accumulation in obesity states.
KeywordsReceptor-interacting protein 140 Morbid obesity Visceral adipose tissue
receptor-interacting protein 140
body fat percentage
body mass index
visceral adipose tissue
This work was supported by FIS PI061458 and PI06/90288 from the Spanish Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo, by the Department of Health of the Gobierno de Navarra (20/2005), and by the Department of Education of the Gobierno de Navarra (res228/2008) of Spain. The authors gratefully acknowledge the valuable collaboration of all the members of the Multidisciplinary Obesity Team. CIBER Fisiopatología de la Obesidad y Nutrición is an initiative of ISCIII.
The authors have nothing to disclose.
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