Sleeve Gastrectomy Relieves Steatohepatitis in High-Fat-Diet-Induced Obese Rats
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Sleeve gastrectomy is thought to decrease the appetite and body weight of morbid obesity patients in the clinic. The purpose is to investigate the effect of sleeve gastrectomy on preventing steatohepatitis in morbid obesity rats.
Thirty rats were randomized into normal chow group (NC), high-fat-diet group (HD), and sleeve group (SG). Rats in the SG group received sleeve gastrectomy operation. After operation, rats in SG and HD group received a high-fat diet, while rats in the NC group received normal chow. Body weight was measured every 10 days. Thirty days later, animals were sacrificed and blood samples were collected to check total cholesterol, HDL, and triglyceride. Fresh liver sections were made and stained with Nile red and observed under a fluorescence microscope.
Rats in the SG group received a moderate body weight decrease (191 ± 16.2 g) in the first 10 days, while this did not happen in the other two groups (213 ± 13.7 g and 243 ± 11.9 g). At the sacrifice date, weight of rats in the SG group was still much lower than those in the HD group. Plasma triglycerol (102.3 ± 18.6 mg/dL) and cholesterol (84.3 ± 6.1 mg/dL) of rats in the SG group were much lower than those in the HD group (198.5 ± 18.5 mg/dL, 133.9 ± 22.0 mg/dL). Under the fluorescence microscope, adipose infiltration was very obvious in the liver of the HD animals, while adipose infiltration was not serious in the SG group.
High-fat diet can result in obvious body weight increase and hepatic adipose infiltration compared with normal chow. Sleeve gastrectomy can decrease body weight even in high-fat-diet models. Body weight control caused by sleeve gastrectomy can relieve high-fat-diet-induced steatohepatitis in rats.
KeywordsBariatric surgery Nile red Sleeve gastrectomy Steatohepatitis Morbid obesity
- 5.Yuan M, Konstantopoulos N, Lee J, et al. Reversal of obesity- and diet-induced insulin resistance with salicylates or targeted disruption of IKKbeta. Science (New York, NY). 2001;293:1673–77.Google Scholar
- 12.Hill JO, Wyatt HR, Reed GW, et al. Obesity and the environment: where do we go from here? Science (New York, NY). 2003;299:853–5.Google Scholar
- 17.Deitel M, Crosby RD, Gagner M. The First International Consensus Summit for Sleeve Gastrectomy (SG), New York City, October 25–27, 2007. Obes Surg. 2008.Google Scholar
- 27.Kotidis EV, Koliakos G, Papavramidis TS, et al. The effect of biliopancreatic diversion with pylorus-preserving sleeve gastrectomy and duodenal switch on fasting serum ghrelin, leptin and adiponectin levels: is there a hormonal contribution to the weight-reducing effect of this procedure? Obes Surg. 2006;16:554–9.PubMedCrossRefGoogle Scholar
- 32.Karamanakos SN, Vagenas K, Kalfarentzos F, et al. Weight loss, appetite suppression, and changes in fasting and postprandial ghrelin and peptide-YY levels after Roux-en-Y gastric bypass and sleeve gastrectomy: a prospective, double blind study. Ann Surg. 2008;247:401–407.PubMedCrossRefGoogle Scholar