Obesity Surgery

, Volume 18, Issue 10, pp 1246–1250 | Cite as

Gastric Bypass Surgery in Rats Produces Weight Loss Modeling after Human Gastric Bypass

  • David S. Tichansky
  • John D. BoughterJr.
  • Jason Harper
  • A. Rebecca Glatt
  • Atul K. Madan
Research Article



The study of the mechanisms of weight loss after bariatric surgery requires an animal model that mimics the human procedure and subsequent weight loss. A rat model eliminates the cognitive efforts associated with human weight loss and gain.


A technique for gastric bypass (Roux-en-Y gastric bypass [RYGB]) was developed in Sprague–Dawley rats. A 1- to 2-cc pouch is created from the uppermost stomach using a linear stapler. A 10-cm biliopancreatic limb and 15-cm Roux limb are anastomosed side to side with running nonabsorbable suture. The gastrojejunostomy is created with a single layer of running nonabsorbable suture. Four rats underwent RYGB. Weight loss was compared to four sham rats that had a midline incision and left 60 min with an open abdomen before closure.


RYGB rats lost an average of 16.5% body weight (BW) at 1 week, 22% BW at 2 weeks, 20% BW at 3 weeks, and 11% BW at 4 weeks. The RYGB rat’s weight was basically level after 4 weeks. The shams lost an average of 4% BW at 1 week, 1% BW at 2 weeks, and 0% BW at 3 weeks and gained an average of 2% at weeks. Subjectively, the RYGB rats were less interested in chow and frequently had chow left in their cage.


A Sprague–Dawley rat model for gastric bypass has been developed and yields approximately 11% BW loss. This will allow investigators to objectively view factors associated with weight loss without the confounding cognitive factors in humans.


Gastric bypass Rat model Weight loss 


  1. 1.
    Maggard MA, Shugarman LR, Suttorp M, et al. Meta-analysis: surgical treatment of obesity. Ann Intern Med. 2005;142:547–59.PubMedGoogle Scholar
  2. 2.
    Buchwald H, Avidor Y, Braunwald E, et al. Bariatric surgery: a systematic review and meta-analysis. JAMA. 2004;292:1724–37.PubMedCrossRefGoogle Scholar
  3. 3.
    Sjostrom L, Lindroos AK, Peltonen M, et al. Lifestyle, diabetes, and cardiovascular risk factors 10 years after bariatric surgery. N Engl J Med. 2004;351:2683–93.PubMedCrossRefGoogle Scholar
  4. 4.
    Fisher BL, Schauer P. Medical and surgical options in the treatment of severe obesity. Am J Surg. 2002;184:9S–16S.PubMedCrossRefGoogle Scholar
  5. 5.
    NIH conference. Gastrointestinal surgery for severe obesity. Consensus Development Conference Panel. Ann Intern Med. 1991;115:956–61.Google Scholar
  6. 6.
    Tichansky DS, Boughter JD Jr, Madan AK. Taste change after laparoscopic Roux-en-Y gastric bypass and laparoscopic adjustable gastric banding. Surg Obes Relat Dis. 2006;2:440–4.PubMedCrossRefGoogle Scholar
  7. 7.
    Bray GA, Barry RE, Benfield JR, et al. Intestinal bypass surgery for obesity decreases food intake and taste preferences. Am J Clin Nutr. 1976;29:779–83.PubMedGoogle Scholar
  8. 8.
    Burge JC, Schaumburg JZ, Choban PS, et al. Changes in patients’ taste acuity after Roux-en-Y gastric bypass for clinically severe obesity. J Am Diet Assoc. 1995;95:666–70.PubMedCrossRefGoogle Scholar
  9. 9.
    Scruggs DM, Buffington C, Cowan GS Jr. Taste acuity of the morbidly obese before and after gastric bypass surgery. Obes Surg. 1994;4:24–8.PubMedCrossRefGoogle Scholar
  10. 10.
    Sclafani A. Appetitive behavior after jejunoileal bypass. Int J Obes. 1981;5:449–55.PubMedGoogle Scholar
  11. 11.
    Meguid MM, Ramos EJ, Suzuki S, et al. A surgical rat model of human Roux-en-Y gastric bypass. J Gastrointest Surg. 2004;8:621–30.PubMedCrossRefGoogle Scholar
  12. 12.
    Quinn R. Comparing rat’s to human’s age: how old is my rat in people years? Nutrition. 2005;21:775–7.PubMedCrossRefGoogle Scholar
  13. 13.
    Suzuki S, Ramos EJ, Goncalves CG, et al. Changes in GI hormones and their effect on gastric emptying and transit times after Roux-en-Y gastric bypass in rat model. Surgery. 2005;138:283–90.PubMedCrossRefGoogle Scholar
  14. 14.
    Xu Y, Ohinata K, Meguid MM, et al. Gastric bypass model in the obese rat to study metabolic mechanisms of weight loss. J Surg Res. 2002;107:56–63.PubMedGoogle Scholar
  15. 15.
    Nijhuis J, van Dielen FM, Buurman WA, et al. Ghrelin, leptin and insulin levels after restrictive surgery: a 2-year follow-up study. Obes Surg. 2004;14:783–7.PubMedCrossRefGoogle Scholar
  16. 16.
    Christou NV, Look D, McLean AP. Pre- and post-prandial plasma ghrelin levels do not correlate with satiety or failure to achieve a successful outcome after Roux-en-Y gastric bypass. Obes Surg. 2005;15:1017–23.PubMedCrossRefGoogle Scholar
  17. 17.
    Korner J, Bessler M, Cirilo LJ, et al. Effects of Roux-en-Y gastric bypass surgery on fasting and postprandial concentrations of plasma ghrelin, peptide YY, and insulin. J Clin Endocrinol Metab. 2005;90:359–65.PubMedCrossRefGoogle Scholar
  18. 18.
    Newman E, O’Connor DB, Conner M. Daily hassles and eating behaviour: the role of cortisol reactivity status. Psychoneuroendocrinology. 2007;32:125–32.PubMedCrossRefGoogle Scholar
  19. 19.
    Goldfield GS, Legg C. Dietary restraint, anxiety, and the relative reinforcing value of snack food in non-obese women. Eat Behav. 2006;7:323–32.PubMedCrossRefGoogle Scholar
  20. 20.
    Masheb RM, Grilo CM. Emotional overeating and its associations with eating disorder psychopathology among overweight patients with binge eating disorder. Int J Eat Disord. 2006;39:141–6.PubMedCrossRefGoogle Scholar
  21. 21.
    Hainer V, Kabrnova K, Aldhoon B, et al. Serotonin and norepinephrine reuptake inhibition and eating behavior. Ann N Y Acad Sci. 2006;1083:252–69.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • David S. Tichansky
    • 1
  • John D. BoughterJr.
    • 2
  • Jason Harper
    • 1
  • A. Rebecca Glatt
    • 2
  • Atul K. Madan
    • 3
  1. 1.Section of Minimally Invasive Surgery, Department of Surgery, College of MedicineUniversity of Tennessee Health Science CenterMemphisUSA
  2. 2.Department of Anatomy and Neurobiology, College of MedicineUniversity of Tennessee Health Science CenterMemphisUSA
  3. 3.Division of Laparoendoscopic and Bariatric SurgeryUniversity of Miami Miller School of MedicineMiamiUSA

Personalised recommendations