Simultaneous optimization of ultrasound-assisted extraction of antioxidants and tyrosinase inhibitory activities of Semen Oroxyli flavonoids using response surface methodology
- 2 Downloads
In this study, the ultrasound-assisted extraction (UAE) of flavonoids with maximal antioxidant and tyrosinase inhibitory activities and identification of the principal individual flavonoids compounds of Semen Oroxyli by UHPLC-DAD-QTOF-MS/MS was proposed. The method was optimized by a three-factor-three-level Box–Behnken design (BBD) and Derringer’s desirability function using methanol concentration, extraction time, and liquid/solid ratio as factors, while, the total flavonoids content (TFC), antioxidant activities (DPPH, ABTS, FRAP, superoxide anion and hydroxyl radicals) and tyrosinase inhibitory activity as responses. The experimental results showed that the optimal extraction conditions were as followed: a solvent of 69.90% methanol, an extraction time of 30.64 min and a liquid/solid ratio of 31.30 mL/g, which give individual response values of 19.79 mg RE/g, 78.66%, 99.92%, 19.05 mM FE/g, 20.49%, and 13.37% for TFC, DPPH, ABTS, FRAP, superoxide anion and hydroxyl radicals, respectively. In addition, the tyrosinase inhibition assay showed that the extract of Semen Oroxyli exhibited strong inhibitory activity against the tyrosinase. There were no significant differences between the results of the optimization experiments and those predicted by the models. By UHPLC-DAD-QTOF-MS/MS analysis, the main flavonoids identified in the optimized UAE extracts of Semen Oroxyli were oroxin B, oroxin A, chrysin-7-O-β- d-glucuronid, baicalein and chrysin.
KeywordsSemen oroxyli Ultrasound-assisted extraction (UAE) Antioxidant activity Tyrosinase inhibition Response surface methodology (RSM) UHPLC-DAD-QTOF-MS/MS
This work was supported by the National Natural Science Foundation of China (81603066), Talent Training Project of Hebei Province (A201902012) and the Scientific Research Funds of the Second Hospital of Hebei Medical University (2h2019031).
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- 1.M.M. Joe, A. Benson, M. Ayyanar, T. Sa, Bioactive Compounds and Medical Significance of Some Endangered Medicinal Plants from the Western Ghats Region of India Biotechnology of Bioactive Compounds: Sources and Applications (Wiley, Hoboken, 163, 2015)Google Scholar
- 2.National Commission of Chinese Pharmacopoeia, Pharmacopoeia of People’s Pepublic of China (China Medical Sciences Press, Beijing, 2010), p. 60Google Scholar
- 7.J. SatyaEswari, S. Dhagat, S. Naik, S. Dibya, Pharm. Bioprocess. 6, 7 (2018)Google Scholar
- 25.R.H. Myers, D.C. Montgomery, C.M. Anderson-Cook, Response Surface Methodology: Process and Product Optimization Using Designed Experiments (Wiley, Hoboken, 2016)Google Scholar
- 38.D.L. Hu, M.J. Wu, Lishizhen Med. Mat Med. Res. 21, 2475 (2010)Google Scholar
- 39.K. Liu, Y. Lan, N. Yang, Z. Chen, W. Zhang, Northwest Pharm. J. 30, 669 (2015)Google Scholar
- 40.Y.G. Li, Study on extraction-isolation and pharmacological activities of total flavonoids from Oroxylun indicum (L) vent (D), University of South China, 2014, pp. 8–10Google Scholar
- 41.Q.X. Guan, S.L. Sun, G.Y. Zhang, S.Y. Zhang, C. Sun, M. Wang, C.C. Han, X. Liu, Spec. Wild Econ. Anim. Plant. Res. 2, 20 (2016)Google Scholar
- 42.Y. Wu, W.J. Bai, X. Wei, Chin. J. Exper Trad Med Formul. 24, 99 (2018)Google Scholar