Structural characterization and antitumor activity of a novel polysaccharide from Grifola frondosa

  • Xiaoyu Chen
  • Haiyu Ji
  • Chaofeng Zhang
  • Juan Yu
  • Anjun LiuEmail author
Original Paper


In this study, a novel GFP-A polysaccharide was extracted from Grifola frondosa, and its structural characteristics and in vivo anti-tumor activities were investigated. The contents of total sugar, uronic acid and protein in GFP-A were 90.18% ± 0.15%, 1.43% ± 0.08% and 0.46% ± 0.03%, respectively. By gas chromatography (GC), Monosaccharide analysis showed that GFP-A (about 2484 kDa) was composed of Rha, Xyl, Man, Glc and Gal with molar percentages of 25.98, 9.32, 11.73, 4.74, and 48.22%. FT-IR and NMR analysis also revealed that GFP-A was a neutral polysaccharide mainly composed of α-type rhamnopyranose, β-type mannopyranose and α-type galactopyranose. Besides, GFP-A could enhance the immunostimulatory, and obviously increase the spleen and thymus indices, spleen lymphocyte proliferation, phagocytic index and NK cells killing activity in tumor-mice. By Flow cytometry (FACS), GFP-A treatment could increase cytotoxic activity of CD4+ and CD8+ T cells, and activate helper T cells to play central roles in immunomodulation of tumor-bearing mice. These results suggest that GFP-A has antitumor activity, which could be explored as a novel natural immunomodulatory agent and as a therapeutic compound to lessen chemotherapy.


Grifola frondosa Polysaccharides Purification Structural characterization Antitumor activity 



This work was supported by QingYunTang Biotech (Beijing) Co., Ltd.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Xiaoyu Chen
    • 1
    • 2
  • Haiyu Ji
    • 1
  • Chaofeng Zhang
    • 1
  • Juan Yu
    • 2
  • Anjun Liu
    • 1
    Email author
  1. 1.A Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and BiotechnologyTianjin University of Science and TechnologyTianjinPeople’s Republic of China
  2. 2.QingYunTang Biotech(Beijing) Co., Ltd.BeijingPeople’s Republic of China

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