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Journal of Food Measurement and Characterization

, Volume 13, Issue 4, pp 3015–3024 | Cite as

Potent antiproliferative and pro-apoptotic effects of a soluble protein fraction from culinary-medicinal mushroom Lentinus tigrinus on cancer cells

  • Safoora Mohammadnejad
  • Hamid R. PourianfarEmail author
  • Ahmad Drakhshan
  • Isa Jabaleh
  • Majid Rezayi
Original Paper
  • 15 Downloads

Abstract

Lentinus tigrinus is a culinary-medicinal mushroom that possesses nutritious substances and biological activities. This study for the first time reports the anticancer potentiality of a soluble protein fraction of L. tigrinus (designated as LTPp) precipitated with polyethylene glycol 6000 on MCF-7 and PC3 cancerous cell lines. The findings of antiproliferation assays revealed that LTPp caused significant inhibitory effects at concentrations less than 1000 µg/mL on both cell lines. Further microscopic observations confirmed obvious morphological changes caused by LTPp in both cell lines. While LTPp generated an IC50 value of 193.5 ± 25 μg/mL in MCF-7 cells, it was 33.60 ± 9.0 μg/mL in PC3 cells. In addition, the mode of cell death detected by co-staining of V-FITC/PI indicated that LTPp at 70 μg/mL killed approximately 45.00 and 70.00% of MCF-7 and PC3 cells, respectively, through inducing apoptosis in these cells after 6 h of treatment. Altogether, LTPp showed greater antiproliferative and cytotoxic activities against PC3 cells compared with MCF-7 cells (p ≤ 0.05). The data presented here suggest that the LTPp fraction may be considered as a potent anticancer compound, which promisingly warrants further studies to isolate and identify active single protein/peptides.

Keywords

Lentinus tigrinus Antiproliferative activity Medicinal mushroom Pro-apoptotic effects Soluble protein Polyethylene glycol 

Notes

Acknowledgements

This research was part of the master thesis project of S Mohammadnejad in ACECR-Khorasan Razavi, Kashmar Higher Education Institute, Kashmar, Iran. The study was partially funded by an internal grant to HR Pourianfar from Industrial Fungi Biotechnology Research Department, ACECR- Khorasan Razavi Branch, Iran. The authors appreciate Dr. Alireza Majid Ansari from Motamed Cancer Institute (Breast Cancer Research Center), Tehran-Iran for his kind assistance with the apoptosis analysis.

Compliance with ethical standards

Conflict of interest

There is no conflict of interest. The authors alone are responsible for the content and writing of the paper.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Plant ProtectionAcademic Center for Education, Culture and Research (ACECR)-Khorasan Razavi, Kashmar Higher Education InstituteKashmarIran
  2. 2.Industrial Fungi Biotechnology Research Department, Research Institute for Industrial BiotechnologyAcademic Center for Education, Culture and Research (ACECR)- Khorasan Razavi BranchMashhadIran
  3. 3.Medical Toxicology Research CenterMashhad University of Medical SciencesMashhadIran
  4. 4.Department of Medical Biotechnology and Nanotechnology, School of MedicineMashhad University of Medical SciencesMashhadIran

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