Journal of Food Measurement and Characterization

, Volume 13, Issue 4, pp 2903–2909 | Cite as

Comprehensive determination of seven polyphenols in Eucommia ulmoides and its anti-oxidative stress activity in C. elegans

  • Peilin Hou
  • Qing Wang
  • Wuqin Qi
  • Yanqing ZhangEmail author
  • Junbo XieEmail author
Original Paper


In this study, an efficient HPLC method was developed and validated to determine seven polyphenols, i.e. chlorogenic acid, caffeic acid, protocatechuic acid, geniposide, rutin, quercetin and isorhamnetin, in different parts (leaves, barks and fruits) of Eucommia ulmoides. A Luna C18 column (150 mm × 3.9 mm, 5.0 μm) was applied and eluted with a gradient of acetonitrile-modified aqueous mobile phase (containing 1% acetic acid). 260 nm, 326 nm, and 360 nm were used as the detection wavelengths. With the developed method, these seven polyphenols were successfully assayed and their contents in different parts of E. ulmoides were compared. The results showed that these polyphenols displayed different distribution in the leaves, barks and fruits. Chlorogenic acid, caffeic acid and rutin were their main common constituents. ABTS and DPPH free radical scavenging assay indicated that E. ulmoides displayed significant antioxidant activities in vitro. In addition, the extracts of the three parts can significantly increase the anti-oxidative stress level in wild-type C. elegans and prolong its lifespan, further demonstrating the antioxidant activity of Eucommia ulmoides.


Eucommia ulmoides Chlorogenic acid Caffeic acid Rutin Anti-oxidation C. elegans 



This work was supported by “131’’ innovative talents training project in Tianjin and the Natural Science Funds of Tianjin (No. 17JCQNJC06000).


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Biotechnology and Food ScienceTianjin University of CommerceTianjinChina
  2. 2.School of Chinese Materia MedicaTianjin University of Traditional Chinese MedicineTianjinChina

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