Journal of Food Measurement and Characterization

, Volume 13, Issue 4, pp 2686–2695 | Cite as

Chemical constituents of Porodaedalea pini mushroom with cytotoxic, antioxidant and anticholinesterase activities

  • Ebru Deveci
  • Gülsen Tel-Çayan
  • Mehmet Emin DuruEmail author
  • Mehmet Öztürk
Original Paper


Chemical investigation of Porodaedalea pini led to the isolation of campesterol (1), ergosta-7,24(28)-dien-3β-ol (2), dioctyl phthalate (3), ergosterol peroxide (4), pinoresinol (5) and 4-(3,4-dihydroxyphenyl)but-3-en-2-one (6). Compounds 1, 3, and 6 were isolated for the first time from P. pini. The structures of compounds were elucidated by IR, 1D-NMR, and 2D-NMR techniques. Antioxidant, anticholinesterase, and cytotoxic activities against breast cancer cell (MCF-7) were tested. The highest antioxidant and cytotoxic activity were found in the methanol extract. Also, compound 6 was found to be active in all antioxidant tests. The hexane extract (38.15 ± 1.50%) exhibited the highest activity against AChE enzyme while the acetone extract (48.75 ± 0.13%) against BChE enzyme. Moreover, among isolated compounds, compound 5 was found to have the highest cytotoxic (IC50: 21.08 ± 1.01 µg/mL), AChE (13.73 ± 0.85%) and BChE (80.02 ± 0.73%) inhibitory activities. The phenolic profile was analyzed by HPLC–DAD and p-hydroxybenzoic acid (32.40 µg/g) was identified as a major compound.


Porodaedalea pini Isolation Antioxidant activity Cytotoxic activity Enzyme inhibitory activity Breast cancer 



2,2′-azino bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt






Butylated hydroxyl anisole


Correlation spectroscopy


Cupric reducing antioxidant capacity




Ethylenediaminetetraacetic acid


Ethyl acetate




Fourier-transform infrared spectroscopy


Heteronuclear multiple bond correlation


High performance liquid chromatography-diode array detection


Heteronuclear single quantum coherence


Half-maximal inhibitory concentration


Nuclear magnetic resonance


Thin layer chromatography



This study is a part of E.D.’s Ph.D. thesis. The authors would like to thank the Scientific and Technological Research Council of Turkey for financial support under project TUBITAK-114Z550. The Mugla Sitki Kocman University Research Fund is also acknowledged under project number (MUBAP 15/238).

Compliance with ethical standards

Conflict of interest

No potential conflict of interest was reported by the authors.

Supplementary material

11694_2019_189_MOESM1_ESM.docx (2 mb)
Supplementary material 1 (DOCX 1999 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of SciencesMuğla Sıtkı Koçman UniversityMuğlaTurkey
  2. 2.Department of Chemistry and Chemical Processing Technologies, Muğla Vocational SchoolMuğla Sıtkı Koçman UniversityMuğlaTurkey

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