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Phytochemical composition and antioxidant activity of 63 Balkan pepper (Capsicum annuum L.) accessions

  • Petko DenevEmail author
  • Velichka Todorova
  • Manol Ognyanov
  • Yordan Georgiev
  • Irina Yanakieva
  • Ivanka Tringovska
  • Stanislava Grozeva
  • Dimitrina Kostova
Original Paper
  • 28 Downloads

Abstract

In our study, we used the rich biodiversity of peppers in the Balkan region and characterized 63 pepper accessions from five cultivar types (Pungent; Sweet; Pumpkin; Kapia; For powder), in view of their phytochemical composition and antioxidant activity. The obtained results showed high variation in the compositional profiles between studied cultivar groups, as well within individual groups. Based on fresh weight (FW), the most abundant sugar in the studied accessions was glucose (0.36–3.79%), followed by fructose (0.16–2.98%). The accessions from “For powder” group were the richest source of uronic acids—0.77%, followed by accessions from “Pungent” group—0.64%. In four of the five groups, citric acid was the predominant organic acid, whereas only in the “Sweet” group, malic acid was the major representative of organic acid. The highest amount of ascorbic acid (Vitamin C) was found in a genotype CAPS-7, which is in the group of “Pungent” accessions. The polyphenol content varied in a broad range from 42 mg/100 g FW to 266 mg/100 g FW. The differences in the flavonoid content were even more significant reaching 60-fold difference—from 1.0 mg/100 g FW to 64.3 mg/100 g FW. The accessions from the “For powder” group were leaders in regards to their total polyphenol and total flavonoid contents, as well as for their antioxidant activity, measured by ORAC and HORAC assays. This variation in fruit composition might be manipulated via plant breeding to create new pepper varieties with improved nutritional value of their fruits.

Keywords

Capsicum annuum L. Sugars Organic acids Polyphenols Pectin Antioxidant activity 

Notes

Acknowledgements

The research leading to these results has received funding from the National Science Fund, Bulgaria [Grant DN06/4]. The authors VT, IT, SG and DK acknowledge the financial support by Horizon 2020 PlantaSYST project under Grant Agreement No 739582.

Funding

This study was funded by the National Science Fund of Bulgaria [Grant DN06/4]. The authors VT, IT, SG and DK acknowledge the financial support by Horizon 2020 PlantaSYST project under Grant Agreement No 739582.

Supplementary material

11694_2019_171_MOESM1_ESM.docx (20 kb)
Supplementary file1 (DOCX 20 kb)
11694_2019_171_MOESM2_ESM.docx (23 kb)
Supplementary file2 (DOCX 23 kb)
11694_2019_171_MOESM3_ESM.docx (25 kb)
Supplementary file3 (DOCX 25 kb)

References

  1. 1.
    FAO, The future of food and agriculture—trends and challenges (FAO, 2017). https://www.fao.org/3/a-i6583e.pdf. Accessed 17 May 2019
  2. 2.
    B. Ou, D. Huang, M. Hampsch-Woodill, J.A. Flanagan, E.K. Deemer, J. Agric. Food Chem. 50, 3122 (2002)CrossRefGoogle Scholar
  3. 3.
    I. Guzman, S. Hamby, J. Romero, P.W. Bosland, M.A. Oçonnell, Plant Sci. 179, 49 (2010)CrossRefGoogle Scholar
  4. 4.
    S. Bogusz Jr., S.H. Libardi, F.F. Dias, J.P. Coutinho, V.C. Bochi, D.R. Arlete, M.T. Melo, H.T. Godoy, J. Sci. Food Agric. 98, 217 (2018)CrossRefGoogle Scholar
  5. 5.
    C.J. Sandoval-Castro, M. Valdez-Morales, B.D. Oomah, R. Gutiérrez-Dorado, S. Medina-Godoy, L.G. Espinosa-Alonso, J. Food Sci. Technol. 54, 1999 (2017)CrossRefGoogle Scholar
  6. 6.
    P.W. Bosland, E.J. Votava, Peppers: Vegetable and Spice Capsicums, 2nd edn. (CABI Publishing, New York, 2000), pp. 1–14Google Scholar
  7. 7.
    J.R. Stommel, E. Albrecht, in Peppers: Botany, Production and Uses, ed. by V.M. Russo (CAB International, Cambridge 2012) p. 29Google Scholar
  8. 8.
    L. Krasteva, Ts. Stoilova, K. Varbanova, St. Neykov, in European Landraces On-Farm Conservation, Management and Use, ed. by M. Veteläinen, V. Negri, N. Maxted N. (Bioversity International, Rome, 2009), p 53Google Scholar
  9. 9.
    Y. Wahyuni, A.-R. Ballester, E. Sudarmonowati, R.J. Bino, A.G. Bovy, Phytochem. 72, 1358 (2011)CrossRefGoogle Scholar
  10. 10.
    W.Y. Liu, W.H. Kang, B.C. Kang, in Genetics, genomics and breeding of peppers and eggplants, ed. by B.-C. Kang, C. Kole (CRC Press Taylor and Francis Group, Boca Raton, 2013), p. 1Google Scholar
  11. 11.
    L.R. Howard, S.T. Talcott, C.H. Brenes, B. Villalon, J. Agric. Food Chem. 48, 1713 (2000)CrossRefGoogle Scholar
  12. 12.
    M.H. Gnayfeed, H.G. Daood, P.A. Biacs, C.F. Alcaraz, J. Sci. Food Agr. 81, 1580 (2001)CrossRefGoogle Scholar
  13. 13.
    J.L. Guil-Guerrero, C. Martinez-Guirado, M. Rebolloso-Fuentes, A. Carrique-Perez, Eur. Food Res. Technol. 224, 1 (2006)CrossRefGoogle Scholar
  14. 14.
    N. Deepa, C. Kaur, B. George, B. Singh, H.C. Kapoor, LWT-Food. Sci. Technol. 40, 121 (2007)Google Scholar
  15. 15.
    Y. Wahyuni, A.-R. Ballester, Y. Tikunov, R.C.H. de Vos, K.T.B. Pelgrom, A. Maharijaya, E. Sudarmonowati, R.J. Bino, A.G. Bovy, Metabolomics 9, 130 (2013)CrossRefGoogle Scholar
  16. 16.
    IPGRI, AVRDC and CATIE, Descriptors for Capsicum (Capsicum spp) (International Plant Genetic Resources Institute, Rome, 1995), pp. 43–44Google Scholar
  17. 17.
    P. Denev, M. Kratchanova, I. Petrova, D. Klisurova, Y. Georgiev, M. Ognyanov, I. Yanakieva, J. Chem. 2018, Article ID 9574587, 13 pages (2018)Google Scholar
  18. 18.
    V. Singleton, J. Rossi, Am. J. Enol. Vitic. 16, 144 (1965)Google Scholar
  19. 19.
    C. Chang, M. Yang, H. Wen, J. Chern, J. Food Drug Anal. 10, 178 (2002)Google Scholar
  20. 20.
    A.E.R. Ahmed, J.M. Labavitch, J. Food Biochem. 1, 361 (1978)CrossRefGoogle Scholar
  21. 21.
    N. Blumenkrantz, G. Asboe-Hansen, Anal. Biochem. 54, 484 (1973)CrossRefGoogle Scholar
  22. 22.
    B. Ou, M. Hampsch-Woodill, R.L. Prior, J. Agric. Food Chem. 49, 4619 (2001)CrossRefGoogle Scholar
  23. 23.
    P. Denev, M. Ciz, G. Ambrozova, A. Lojek, I. Yanakieva, M. Kratchanova, Food Chem. 123, 1055 (2010)CrossRefGoogle Scholar
  24. 24.
    P.G. Acosta-Quezada, M.D. Raigón, T. Riofrío-Cuenca, M.D. García-Martínez, M. Plazas, J.I. Burneo, J.G. Figueroa, S. Vilanova, J. Prohens, Food Chem. 169, 327 (2015)CrossRefGoogle Scholar
  25. 25.
    G. Corrado, M. Caramante, P. Piffanelli, R. Rao, Sci. Hortic. 168, 138 (2014)CrossRefGoogle Scholar
  26. 26.
    A. Jamiołkowska, H. Buczkowska, A.H. Thanoon, Acta Sci. Pol. Hortorum Cultus 15, 95 (2016)Google Scholar
  27. 27.
    P. Luning, Characterisation of the Flavour of Fresh Bell Peppers (Capsicum annuum) and Its Changes After Hot-Air Drying: An Instrumental and Sensory Evaluation (Wageningen, Wageningen University Press, 1995), pp. 31–46Google Scholar
  28. 28.
    S. Osorio, R. Alba, Z. Nikoloski, A. Kochevenko, A.R. Fernie, J.J. Giovannoni, Plant Physiol. 159, 1713 (2012)CrossRefGoogle Scholar
  29. 29.
    A.G.J. Voragen, W. Pilnik, J.-F. Thibault, M.A.V. Axelos, C.M.G.C. Renard, in Food Polysaccharides and Their Applications, ed. by A.M. Stephen (Marcel Dekker Inc, New York, 1995), p. 287Google Scholar
  30. 30.
    Y.N. Georgiev, M.H. Ognyanov, P.N. Denev, M.G. Kratchanova, in Handbook of Food Bioengineering, Therapeutic Foods, ed. by A.M. Holban, A.M. Grumezescu (Elsevier, Cambridge, 2018), p. 285CrossRefGoogle Scholar
  31. 31.
    J. Lopez-Hernindez, M.J. Oruña-Concha, J. Simal-Lozano, M.E. Vázquez-Blanc, M.J. Gonzáilez-Castro, Food Chem. 57, 557 (1996)CrossRefGoogle Scholar
  32. 32.
    R.L. Jarret, T. Berke, E.A. Baldwin, G.F. Antonious, Chem. Biodivers. 6, 138 (2009)CrossRefGoogle Scholar
  33. 33.
    A.F. Molinari, L.R. Castro, S. Antoniali, P. Pornchaloempong, A.J. Fox, S.A. Sargent, E.M. Lamb, Proc. Florida State Hortic. Soc. p. 143 (1999)Google Scholar
  34. 34.
    M. Ghasemnezhad, M. Sherafati, G.A. Payvast, J. Funct. Foods 3, 44 (2011)CrossRefGoogle Scholar
  35. 35.
    Food and Nutrition Board, Institute of Medicine, National Academies. Dietary Reference Intakes (DRIs): Recommended Dietary Allowances and Adequate Intakes, Vitamins. https://www.ncbi.nlm.nih.gov/books/NBK56068/table/summarytables.t2/?report=objectonly. Accessed 15 May 2019
  36. 36.
    L.R. Howard, R.E.C. Wildman, in Handbook of Nutraceuticals and Functional Foods, ed. by R.E.C. Wildman (CRC Press, Boca Raton, 2007) p. 165Google Scholar
  37. 37.
    S.A. Aliu, I. Rusinovci, S. Fetahu, S. Kaçiu, D. Zeka, Acta Agric. Slov. 109, 205 (2017)CrossRefGoogle Scholar
  38. 38.
    A.O. Kumar, S.S. Tata, Not. Sci. Biol. 1, 50 (2009)CrossRefGoogle Scholar
  39. 39.
    A. Kołton, R. Wojciechowska, M. Leja, Veg. Crop Res. Bull. 74, 143 (2011)Google Scholar
  40. 40.
    V. Perla, P. Nimmakayala, M. Nadimi, S. Alaparthi, G.R. Hankins, A.W. Ebert, U.K. Reddy, Food Chem. 202, 189 (2016)CrossRefGoogle Scholar
  41. 41.
    M. Naczk, F. Shahidi, J. Chromatogr. A 1054, 95 (2004)CrossRefGoogle Scholar
  42. 42.
    A. Crozier, I.B. Jaganath, M.N. Clifford, in Plant Secondary Metabolites: Occurrence, Structure and Role in the Human Diet, ed. by A. Crozier, M.N. Clifford, H. Ashihara (Blackwell Publishing, Oxford, 2006), p. 1CrossRefGoogle Scholar
  43. 43.
    P. Pietta, M. Minoggio, L. Bramati, in Studies in Natural Products Chemistry, ed. A. Rahman (Elsevier, Amsterdam, 2003) p. 257Google Scholar
  44. 44.
    R. Slimestad, M. Verheul, J. Sci. Food Agric. 89, 1255 (2009)CrossRefGoogle Scholar
  45. 45.
    Y. Lee, L.R. Howard, B. Villalón, J. Food Sci. 60, 473 (1995)CrossRefGoogle Scholar
  46. 46.
    A. Marín, F. Ferreres, F.A. Tomás-Barberán, M.I., Gil, J. Agric. Food Chem. 52, 3861 (2004)CrossRefGoogle Scholar
  47. 47.
    M. Číž, H. Čížová, P. Denev, M. Kratchanova, A. Slavov, A. Lojek, Food Control 21, 518 (2010)CrossRefGoogle Scholar
  48. 48.
    P. Denev, A. Lojek, M. Ciz, M. Kratchanova, Bulg. J. Agric. Sci. 19, 22 (2013)Google Scholar
  49. 49.
    P. Denev, M. Číž, M. Kratchanova, D. Blazheva, Food Chem. 284, 108 (2019)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Biologically Active Substances, Institute of Organic Chemistry with Centre of PhytochemistryBulgarian Academy of SciencesPlovdivBulgaria
  2. 2.Maritsa Vegetable Crops Research InstitutePlovdivBulgaria
  3. 3.Center of Plant Systems Biology and BiotechnologyPlovdivBulgaria

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